Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38502 |
Resumo: | Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-1 and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and ?-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-1 of endoglucanases, 2024 U gss-1 of xylanase, 12.6 U gss-1 of FPU and 41 U gss-1 of ?-glucosidase. The results of the application tests indicated the best conditions as 7.0 ml of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose. |
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Semina. Ciências Agrárias (Online) |
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Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasseCelulases fúngicas: produção por cultivo sólido em biorreator de leito empacotado usando resíduos sólidos agroindustriais como substratos e aplicação na hidrólise de bagaço de canaBioethanolCellulasesPretreatmentSaccharificationSolid cultivation.BioetanolCelulasesPré-tratamentoSacarificaçãoCultivo sólido.Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-1 and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and ?-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-1 of endoglucanases, 2024 U gss-1 of xylanase, 12.6 U gss-1 of FPU and 41 U gss-1 of ?-glucosidase. The results of the application tests indicated the best conditions as 7.0 ml of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose.Celulases são essenciais para a hidrólise de materiais lignocelulósicos visando à produção de etanol de segunda geração. O cultivo em estado sólido é um processo que proporciona altas concentrações de enzimas que podem ser aplicadas nessa hidrólise. O objetivo deste trabalho foi produzir celulases pelo fungo Myceliophthora thermophila I-1D3b em biorreator de leito empacotado com bagaço de cana (BC) e farelo de trigo (FT) como substrato e avaliar a eficiência deste extrato enzimático na hidrólise de bagaço de cana in natura (BIN) e pré-tratado com ozônio, álcali e ultrassom (BOU). As condições para produção de enzimas no biorreator foram BC:FT numa proporção de 2,3:1 (m/m); 75 % de umidade em base úmida; 45 ºC; taxa de aeração 240 L h-1 e 96 h. A produção de enzimas foi avaliada pelas atividades de endoglucanase, xilanase, papel de filtro (FPU) e ?-glicosidase. Para a aplicação das enzimas, foi utilizado um planejamento de superfície de resposta central composto com 5 repetições do ponto central, tendo como fatores o volume de extrato enzimático e o tempo de hidrólise. Esse cultivo produziu as seguintes atividades enzimáticas: 723 U gss-1 de endoglucanases, 2024 U gss-1 de xilanase, 12,6 U gss-1 de FPU e 41 U gss-1 de ?-glucosidase. Os resultados da aplicação dessas enzimas na hidrólise indicaram como ótimos 7,0 ml de extrato enzimático (4,2 FPU) e 6 horas para BIN e BOU. As melhores conversões de celulose-glicose foram obtidas para a BOU, atingindo 32,1 % a 65 ºC. Em conclusão, a produção de enzimas no biorreator de leito compactado foi eficiente e o pré-tratamento BOU melhorou a hidrólise da biomassa, aumentando a eficiência de conversão de celulose em glicose.UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionResearch ArticlesArtigo de pesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3850210.5433/1679-0359.2020v41n5supl1p2097Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2097-2116Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2097-21161679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38502/27785Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFrassatto, Priscila Aparecida CasciatoriCasciatori, Fernanda PerpétuaThoméo, João CláudioGomes, EleniBoscolo, MaurícioSilva, Roberto da2022-10-07T13:19:18Zoai:ojs.pkp.sfu.ca:article/38502Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T13:19:18Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse Celulases fúngicas: produção por cultivo sólido em biorreator de leito empacotado usando resíduos sólidos agroindustriais como substratos e aplicação na hidrólise de bagaço de cana |
title |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse |
spellingShingle |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse Frassatto, Priscila Aparecida Casciatori Bioethanol Cellulases Pretreatment Saccharification Solid cultivation. Bioetanol Celulases Pré-tratamento Sacarificação Cultivo sólido. |
title_short |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse |
title_full |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse |
title_fullStr |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse |
title_full_unstemmed |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse |
title_sort |
Fungal cellulases: production by solid-state cultivation in packed-bed bioreactor using solid agro-industrial by-products as substrates and application for hydrolysis of sugarcane bagasse |
author |
Frassatto, Priscila Aparecida Casciatori |
author_facet |
Frassatto, Priscila Aparecida Casciatori Casciatori, Fernanda Perpétua Thoméo, João Cláudio Gomes, Eleni Boscolo, Maurício Silva, Roberto da |
author_role |
author |
author2 |
Casciatori, Fernanda Perpétua Thoméo, João Cláudio Gomes, Eleni Boscolo, Maurício Silva, Roberto da |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Frassatto, Priscila Aparecida Casciatori Casciatori, Fernanda Perpétua Thoméo, João Cláudio Gomes, Eleni Boscolo, Maurício Silva, Roberto da |
dc.subject.por.fl_str_mv |
Bioethanol Cellulases Pretreatment Saccharification Solid cultivation. Bioetanol Celulases Pré-tratamento Sacarificação Cultivo sólido. |
topic |
Bioethanol Cellulases Pretreatment Saccharification Solid cultivation. Bioetanol Celulases Pré-tratamento Sacarificação Cultivo sólido. |
description |
Cellulases are essential for the hydrolysis of lignocellulosic materials in the production of second generation ethanol. Solid-state cultivation is a process that provides high concentrations of enzymes that can be used in this hydrolysis. The objectives of this work were to produce cellulases by cultivating the fungus Myceliophthora thermophila I-1D3b in a packed bed bioreactor with sugarcane bagasse (SCB) and wheat bran (WB) as substrate and to evaluate the efficiency of the enzymatic extract in the hydrolysis of SCB in natura (BIN) and pretreated with ozone, alkali and ultrasound (BOU). The conditions for enzyme production in the bioreactor were SCB:WB at a ratio of 2.3:1 (w/w), 75 % moisture content; 45 ºC; aeration rate 240 L h-1 and 96 h. The enzyme production was evaluated by endoglucanase, xylanase, filter paper (FPU) and ?-glycosidase activities. For the application of the enzymes, a central composed response surface design with 5 repetitions of the central point was used, taking enzyme volume and hydrolysis time as factors. Such cultivation yielded the following enzymatic activities: 723 U gss-1 of endoglucanases, 2024 U gss-1 of xylanase, 12.6 U gss-1 of FPU and 41 U gss-1 of ?-glucosidase. The results of the application tests indicated the best conditions as 7.0 ml of the enzyme extract (4.2 FPU) and 6 hours for BIN and BOU. The best cellulose-glucose conversions were obtained for BOU, reaching 32.1 % at 65 ºC. In conclusion, the enzyme production in the packed bed bioreactor was efficient and BOU pretreatment improved the hydrolysis of biomass, increasing the efficiency of conversion of cellulose to glucose. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Research Articles Artigo de pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38502 10.5433/1679-0359.2020v41n5supl1p2097 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38502 |
identifier_str_mv |
10.5433/1679-0359.2020v41n5supl1p2097 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38502/27785 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2097-2116 Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2097-2116 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306082326151168 |