In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates

Detalhes bibliográficos
Autor(a) principal: Possamai, Monique Catarine Fischer
Data de Publicação: 2019
Outros Autores: Santos, Isabela Carvalho dos, Silva, Eloísa Schneider, Gazim, Zilda Cristiani, Gonçalves, José Eduardo, Soares, Andréia Assunção, Germano, Ricardo de Melo, Fanin, Maurício, Sá, Thaís Camaso de, Otutumi, Luciana Kazue
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988
Resumo: The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials.
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spelling In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolatesAtividade bacteriostática in vitro dos óleos essenciais de Origanum vulgare, Cymbopogon citratus e Lippia alba em isolados bacterianos oriundos de rações de gatosMinimum inhibitory concentrationCarvacrolGeranialResistance.Concentração inibitória mínimaCarvacrolGeranialResistência.The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials.Atualmente, a indústria pet vem se expandido e se especializando principalmente na área de felinos domésticos. Além disso, problemas relacionados à resistência aos antimicrobianos são frequentes e o uso de óleos essenciais (OEs) nas rações destinadas à alimentação animal têm se tornado uma nova estratégia de tratamento. Dessa forma, o objetivo do trabalho foi avaliar a atividade bacteriostática dos OEs de erva-cidreira brasileira (Lippia alba), capim-limão (Cymbopogon citratus) e orégano (Origanum vulgare) em isolados bacterianos oriundos de 12 amostras de rações de gato comercializados a granel. Os OEs foram obtidos das folhas frescas de culturas oriundas do Horto Medicinal da Universidade Paranaense, Umuarama, Paraná. As amostras de ração foram processadas para identificação dos micro-organismos Gram-positivos e Gram-negativos. A determinação da atividade bacteriostática dos OEs foi feita por meio da determinação da concentração inibitória mínima (CIM) para as diluições de 2,5; 1,25 e 0,62 mg/mL. A técnica de disco difusão foi utilizada para avaliar o perfil de resistência aos principais antimicrobianos utilizados na clínica de felinos e para a análise do efeito da associação destes com os OEs estudados. Foram obtidos 23 isolados, dos quais 16 eram gram-negativos e sete gram-positivos. Em relação à composição dos OEs, foram identificados para os óleos de L. alba, C. citratus e O. vulgare, 40, 24 e 44 compostos respectivamente, cujos compostos majoritários foram geranial, geranial/?-citral e carvacrol, respectivamente. Em relação à CIM, não foram verificadas diferenças para nenhum dos OEs testados. O menor valor da CIM foi obtido para OE de C. citratus (0,83 mg/mL) para duas bactérias (Staphylococcus coagulase negativa e Corynebacterium kutscheri). A média dos halos de inibição aos 10 antimicrobianos testados em associação ou não a um dos OEs para K. aerogenes, P. vulgaris e S. rubidaea demonstrou que para a S. rubidaea, houve maior (P < 0,05) diâmetro do halo de inibição (12,4 mm) quando da associação da amoxicilina com OE de O. vulgare em comparação com associação do mesmo antibiótico com o OE de C. citratus (11,0 mm). Para K. aerogenes e P. vulgaris não foram verificadas diferenças no diâmetro do halo de inibição quando da inclusão dos OEs. Conclui-se que os OEs de L. alba, C. citratus e O. vulgare são eficazes na inibição do crescimento de bactérias gram-positivas e gram-negativas, podendo vir a ser introduzidos na ração de felinos em substituição aos antimicrobianos químicos.UEL2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3598810.5433/1679-0359.2019v40n6Supl2p3107Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3107-3122Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3107-31221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988/26135Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPossamai, Monique Catarine FischerSantos, Isabela Carvalho dosSilva, Eloísa SchneiderGazim, Zilda CristianiGonçalves, José EduardoSoares, Andréia AssunçãoGermano, Ricardo de MeloFanin, MaurícioSá, Thaís Camaso deOtutumi, Luciana Kazue2022-10-10T16:13:49Zoai:ojs.pkp.sfu.ca:article/35988Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:13:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
Atividade bacteriostática in vitro dos óleos essenciais de Origanum vulgare, Cymbopogon citratus e Lippia alba em isolados bacterianos oriundos de rações de gatos
title In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
spellingShingle In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
Possamai, Monique Catarine Fischer
Minimum inhibitory concentration
Carvacrol
Geranial
Resistance.
Concentração inibitória mínima
Carvacrol
Geranial
Resistência.
title_short In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
title_full In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
title_fullStr In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
title_full_unstemmed In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
title_sort In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
author Possamai, Monique Catarine Fischer
author_facet Possamai, Monique Catarine Fischer
Santos, Isabela Carvalho dos
Silva, Eloísa Schneider
Gazim, Zilda Cristiani
Gonçalves, José Eduardo
Soares, Andréia Assunção
Germano, Ricardo de Melo
Fanin, Maurício
Sá, Thaís Camaso de
Otutumi, Luciana Kazue
author_role author
author2 Santos, Isabela Carvalho dos
Silva, Eloísa Schneider
Gazim, Zilda Cristiani
Gonçalves, José Eduardo
Soares, Andréia Assunção
Germano, Ricardo de Melo
Fanin, Maurício
Sá, Thaís Camaso de
Otutumi, Luciana Kazue
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Possamai, Monique Catarine Fischer
Santos, Isabela Carvalho dos
Silva, Eloísa Schneider
Gazim, Zilda Cristiani
Gonçalves, José Eduardo
Soares, Andréia Assunção
Germano, Ricardo de Melo
Fanin, Maurício
Sá, Thaís Camaso de
Otutumi, Luciana Kazue
dc.subject.por.fl_str_mv Minimum inhibitory concentration
Carvacrol
Geranial
Resistance.
Concentração inibitória mínima
Carvacrol
Geranial
Resistência.
topic Minimum inhibitory concentration
Carvacrol
Geranial
Resistance.
Concentração inibitória mínima
Carvacrol
Geranial
Resistência.
description The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988
10.5433/1679-0359.2019v40n6Supl2p3107
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988
identifier_str_mv 10.5433/1679-0359.2019v40n6Supl2p3107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988/26135
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3107-3122
Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3107-3122
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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