In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988 |
Resumo: | The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials. |
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In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolatesAtividade bacteriostática in vitro dos óleos essenciais de Origanum vulgare, Cymbopogon citratus e Lippia alba em isolados bacterianos oriundos de rações de gatosMinimum inhibitory concentrationCarvacrolGeranialResistance.Concentração inibitória mínimaCarvacrolGeranialResistência.The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials.Atualmente, a indústria pet vem se expandido e se especializando principalmente na área de felinos domésticos. Além disso, problemas relacionados à resistência aos antimicrobianos são frequentes e o uso de óleos essenciais (OEs) nas rações destinadas à alimentação animal têm se tornado uma nova estratégia de tratamento. Dessa forma, o objetivo do trabalho foi avaliar a atividade bacteriostática dos OEs de erva-cidreira brasileira (Lippia alba), capim-limão (Cymbopogon citratus) e orégano (Origanum vulgare) em isolados bacterianos oriundos de 12 amostras de rações de gato comercializados a granel. Os OEs foram obtidos das folhas frescas de culturas oriundas do Horto Medicinal da Universidade Paranaense, Umuarama, Paraná. As amostras de ração foram processadas para identificação dos micro-organismos Gram-positivos e Gram-negativos. A determinação da atividade bacteriostática dos OEs foi feita por meio da determinação da concentração inibitória mínima (CIM) para as diluições de 2,5; 1,25 e 0,62 mg/mL. A técnica de disco difusão foi utilizada para avaliar o perfil de resistência aos principais antimicrobianos utilizados na clínica de felinos e para a análise do efeito da associação destes com os OEs estudados. Foram obtidos 23 isolados, dos quais 16 eram gram-negativos e sete gram-positivos. Em relação à composição dos OEs, foram identificados para os óleos de L. alba, C. citratus e O. vulgare, 40, 24 e 44 compostos respectivamente, cujos compostos majoritários foram geranial, geranial/?-citral e carvacrol, respectivamente. Em relação à CIM, não foram verificadas diferenças para nenhum dos OEs testados. O menor valor da CIM foi obtido para OE de C. citratus (0,83 mg/mL) para duas bactérias (Staphylococcus coagulase negativa e Corynebacterium kutscheri). A média dos halos de inibição aos 10 antimicrobianos testados em associação ou não a um dos OEs para K. aerogenes, P. vulgaris e S. rubidaea demonstrou que para a S. rubidaea, houve maior (P < 0,05) diâmetro do halo de inibição (12,4 mm) quando da associação da amoxicilina com OE de O. vulgare em comparação com associação do mesmo antibiótico com o OE de C. citratus (11,0 mm). Para K. aerogenes e P. vulgaris não foram verificadas diferenças no diâmetro do halo de inibição quando da inclusão dos OEs. Conclui-se que os OEs de L. alba, C. citratus e O. vulgare são eficazes na inibição do crescimento de bactérias gram-positivas e gram-negativas, podendo vir a ser introduzidos na ração de felinos em substituição aos antimicrobianos químicos.UEL2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3598810.5433/1679-0359.2019v40n6Supl2p3107Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3107-3122Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3107-31221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988/26135Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPossamai, Monique Catarine FischerSantos, Isabela Carvalho dosSilva, Eloísa SchneiderGazim, Zilda CristianiGonçalves, José EduardoSoares, Andréia AssunçãoGermano, Ricardo de MeloFanin, MaurícioSá, Thaís Camaso deOtutumi, Luciana Kazue2022-10-10T16:13:49Zoai:ojs.pkp.sfu.ca:article/35988Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:13:49Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates Atividade bacteriostática in vitro dos óleos essenciais de Origanum vulgare, Cymbopogon citratus e Lippia alba em isolados bacterianos oriundos de rações de gatos |
title |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates |
spellingShingle |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates Possamai, Monique Catarine Fischer Minimum inhibitory concentration Carvacrol Geranial Resistance. Concentração inibitória mínima Carvacrol Geranial Resistência. |
title_short |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates |
title_full |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates |
title_fullStr |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates |
title_full_unstemmed |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates |
title_sort |
In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates |
author |
Possamai, Monique Catarine Fischer |
author_facet |
Possamai, Monique Catarine Fischer Santos, Isabela Carvalho dos Silva, Eloísa Schneider Gazim, Zilda Cristiani Gonçalves, José Eduardo Soares, Andréia Assunção Germano, Ricardo de Melo Fanin, Maurício Sá, Thaís Camaso de Otutumi, Luciana Kazue |
author_role |
author |
author2 |
Santos, Isabela Carvalho dos Silva, Eloísa Schneider Gazim, Zilda Cristiani Gonçalves, José Eduardo Soares, Andréia Assunção Germano, Ricardo de Melo Fanin, Maurício Sá, Thaís Camaso de Otutumi, Luciana Kazue |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Possamai, Monique Catarine Fischer Santos, Isabela Carvalho dos Silva, Eloísa Schneider Gazim, Zilda Cristiani Gonçalves, José Eduardo Soares, Andréia Assunção Germano, Ricardo de Melo Fanin, Maurício Sá, Thaís Camaso de Otutumi, Luciana Kazue |
dc.subject.por.fl_str_mv |
Minimum inhibitory concentration Carvacrol Geranial Resistance. Concentração inibitória mínima Carvacrol Geranial Resistência. |
topic |
Minimum inhibitory concentration Carvacrol Geranial Resistance. Concentração inibitória mínima Carvacrol Geranial Resistência. |
description |
The pet industry is currently expanding and specializing mainly in the field of domestic felines. Problems related to antimicrobial resistance are frequent, and the use of essential oils (EOs) in animal feed has become a novel treatment strategy. Thus, the objective of this study was to assess the bacteriostatic activity of Brazilian lemon balm (Lippia alba), lemon grass (Cymbopogon citratus), and oregano (Origanum vulgare) in bacterial isolates from 12 samples of cat food sold in bulk. The EOs from fresh leaves of crops were obtained from the Medicinal Garden of Paranaense University, Umuarama, Paraná. Cat food samples were processed for identification of gram-positive and gram-negative microorganisms. The determination of the bacteriostatic activity of the EOs was performed by determination of the minimum inhibitory concentration (MIC) at dilutions of 2.5, 1.25, and 0.62 mg/mL. The diffusion disc technique was used to evaluate the resistance profile to the main antimicrobials used in the feline clinic and to analyze the effect of the association of these antimicrobials with the EOs studied. A total of 23 isolates were obtained, of which 16 were gram-negative and seven were gram-positive. As for the oil composition for L. alba, C. citratus, and O. vulgare, 40, 24, and 44 compounds were identified, respectively, with the major ones being geranial, geranial/?-citral, and carvacrol, respectively. Regarding MIC, no differences were found for any EOs tested. The lowest MIC value was obtained for the C. citratus EO (0.83 mg/mL) for two bacteria (coagulase-negative Staphylococcus and Corynebacterium kutscheri). The means of the inhibition halos for the 10 antimicrobials tested in association or not with one of the EOs for Klebsiella aerogenes, Proteus vulgaris, and Serratia rubidaea showed that, for S. rubidaea, the inhibition halo diameter (12.4 mm) was greater (p < 0.05) when amoxicillin was associated with the O. vulgare EO than the association of the same antibiotic with the C. citratus EO (11.0 mm). For K. aerogenes and P. vulgaris, there was no difference in inhibition halo diameter when EOs were included. In conclusion, L. alba, C. citratus, and O. vulgare EOs are effective in inhibiting the growth of gram-positive and gram-negative bacteria and can be added to cat food to replace chemical antimicrobials. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988 10.5433/1679-0359.2019v40n6Supl2p3107 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6Supl2p3107 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35988/26135 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3107-3122 Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3107-3122 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306081085685760 |