Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Semina. Ciências Agrárias (Online) |
| Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48870 |
Resumo: | This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes. |
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Semina. Ciências Agrárias (Online) |
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Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral folliclesEfeito do extrato acidificado das folhas de Moringa oleifera como suplemento no meio de cultivo in vitro de folículos pré-antrais ovinosPhenolic compoundMoringa oleiferaOocyteOvinePreantral follicle.Composto fenólicoFolículo pré-antralMoringa oleiferaOócitoOvino.This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes.: Este estudo foi conduzido para avaliar os efeitos do extrato acidificado de folhas de M. oleifera como suplemento ao meio base para cultivo in vitro de folículos secundários isolados de ovinos. Os folículos foram isolados e cultivados por 12 dias em α-MEM+ (suplementado com albumina sérica bovina, insulina, glutamina, hipoxantina, transferrina, selênio e ácido ascórbico) com ou sem 0,1; 0,2 ou 0,4 mg/ml do extrato acidificado de M. oleifera. Foram avaliados morfologia folicular, formação da cavidade antral, diâmetro folicular e oocitário, concentração de glutationa (GSH), atividade mitocondrial e retomada meiótica. Após 12 dias de cultivo, não houve diferença significativa entre os tratamentos em relação à morfologia folicular, formação da cavidade antral, diâmetro e atividade mitocondrial. No entanto, oócitos de folículos cultivados em α-MEM+ apresentaram maior concentração de GSH do que meios contendo extrato de M. oleifera. Além disso, a concentração de 0,4 mg/ml de extrato de M. oleifera aumentou significativamente a porcentagem de oócitos totalmente crescidos (≥ 110 µm) quando comparado aos demais tratamentos. Em conclusão, a concentração de 0,4 mg/ml de extrato de M. oleifera como suplemento do meio de cultura manteve a sobrevivência e aumentou a porcentagem de oócitos totalmente crescidos.UEL2023-12-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4887010.5433/1679-0359.2023v44n6p1991Semina: Ciências Agrárias; Vol. 44 No. 6 (2023); 1991-2010Semina: Ciências Agrárias; v. 44 n. 6 (2023); 1991-20101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48870/49788Copyright (c) 2023 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGuimarães, Valéria da SilvaSilva, Regina Lucia dos SantosBarberino, Ricássio de SouzaMota, Istefani MoreiraPinto, Joisyleide Gonçalves CostaTorres, Maria Lilian Gomes LoiolaSilva, Naiane Darklei do SantosQueiroz, Mário Adriano ÁvilaMatos, Maria Helena Tavares deMonte, Alane Pains Oliveira do2024-01-30T13:16:11Zoai:ojs.pkp.sfu.ca:article/48870Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2024-01-30T13:16:11Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
| dc.title.none.fl_str_mv |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles Efeito do extrato acidificado das folhas de Moringa oleifera como suplemento no meio de cultivo in vitro de folículos pré-antrais ovinos |
| title |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles |
| spellingShingle |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles Guimarães, Valéria da Silva Phenolic compound Moringa oleifera Oocyte Ovine Preantral follicle. Composto fenólico Folículo pré-antral Moringa oleifera Oócito Ovino. |
| title_short |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles |
| title_full |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles |
| title_fullStr |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles |
| title_full_unstemmed |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles |
| title_sort |
Effect of the acidified extract of Moringa oleifera leaves as a supplement in the in vitro culture medium of sheep preantral follicles |
| author |
Guimarães, Valéria da Silva |
| author_facet |
Guimarães, Valéria da Silva Silva, Regina Lucia dos Santos Barberino, Ricássio de Souza Mota, Istefani Moreira Pinto, Joisyleide Gonçalves Costa Torres, Maria Lilian Gomes Loiola Silva, Naiane Darklei do Santos Queiroz, Mário Adriano Ávila Matos, Maria Helena Tavares de Monte, Alane Pains Oliveira do |
| author_role |
author |
| author2 |
Silva, Regina Lucia dos Santos Barberino, Ricássio de Souza Mota, Istefani Moreira Pinto, Joisyleide Gonçalves Costa Torres, Maria Lilian Gomes Loiola Silva, Naiane Darklei do Santos Queiroz, Mário Adriano Ávila Matos, Maria Helena Tavares de Monte, Alane Pains Oliveira do |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Guimarães, Valéria da Silva Silva, Regina Lucia dos Santos Barberino, Ricássio de Souza Mota, Istefani Moreira Pinto, Joisyleide Gonçalves Costa Torres, Maria Lilian Gomes Loiola Silva, Naiane Darklei do Santos Queiroz, Mário Adriano Ávila Matos, Maria Helena Tavares de Monte, Alane Pains Oliveira do |
| dc.subject.por.fl_str_mv |
Phenolic compound Moringa oleifera Oocyte Ovine Preantral follicle. Composto fenólico Folículo pré-antral Moringa oleifera Oócito Ovino. |
| topic |
Phenolic compound Moringa oleifera Oocyte Ovine Preantral follicle. Composto fenólico Folículo pré-antral Moringa oleifera Oócito Ovino. |
| description |
This study was conducted to evaluate the effects of the acidified extract of M. oleifera leaves as a supplement into the base medium for in vitro culture of sheep isolated secondary follicles. Follicles were isolated and cultured for 12 days in α-MEM+(supplemented with bovine serum albumin, insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid) with or without 0.1; 0.2 or 0.4 mg/ml of the acidified extract of M. oleifera. Follicle morphology, antral cavity formation, follicular and oocyte diameter, glutathione (GSH) concentration, mitochondrial activity and meiotic resumption were evaluated. After 12 days of culture, there was no significant difference among treatments in relation to follicular morphology, antral cavity formation, diameter and mitochondrial activity. Nevertheless, oocytes from follicles cultured in α-MEM+ showed greater GSH concentration than media containing M. oleifera extract. Furthermore, the concentration of 0.4 mg/ml M. oleifera extract significantly increased the percentage of fully grown oocyte (≥ 110 µm) when compared to the other treatments. In conclusion, the concentration of 0.4 mg/ml M. oleifera extract as a supplement of the culture medium, maintained the survival, and increased the percentage of fully grown oocytes. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-12-18 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48870 10.5433/1679-0359.2023v44n6p1991 |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48870 |
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10.5433/1679-0359.2023v44n6p1991 |
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eng |
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eng |
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https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/48870/49788 |
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Copyright (c) 2023 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
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Copyright (c) 2023 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
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openAccess |
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application/pdf |
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UEL |
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UEL |
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Semina: Ciências Agrárias; Vol. 44 No. 6 (2023); 1991-2010 Semina: Ciências Agrárias; v. 44 n. 6 (2023); 1991-2010 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
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Universidade Estadual de Londrina (UEL) |
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UEL |
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UEL |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) |
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Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
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semina.agrarias@uel.br |
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1799306087256555520 |