Does resveratrol preserve the integrity of the sperm membrane in goats after thawing?
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39326 |
Resumo: | This study aimed to determine the effects of resveratrol in the trisaminomethane (TRIS)-egg yolk extender and its optimal inclusion level for goat semen cryopreservation. Five ejaculates of three Anglo Nubian goats were used, each divided into four 200 µL aliquots for use in four treatments: 0.00 (control), 0.04, 0.08 and 0.12 mg mL-1 resveratrol in the TRIS-egg yolk extender. We evaluated progressive sperm motility and sperm vigor post-dilution, post-cooling, and post thawing; membrane integrity (HOST); and acrosomal integrity and performed a slow thermoresistance test (STT). The data were submitted to a regression analysis at a 5% probability. There was no difference in progressive motility or sperm vigor in the post-dilution (89.5, 89.0, 88.7 and 88.3, and 4.9, 5.0, 4.9, and 4.9) or post-cooling (81.0, 82.0, 83.0, and 78.3; and 4.3, 4.3, 4.2, and 4.2) experiments (P > 0.05), or in the complementary acrosomal integrity test (42.0, 47.4, 42.2 and 38.2) (P > 0.05). However, the motility and vigor parameters decreased linearly in the post-thaw phase, as well as during the 2 hours of incubation on STT (P < 0.05). These factors increased quadratically when resveratrol was added to HOST, to an optimal level of 0.039 mg mL-1 resveratrol for a plasma membrane integrity of 52.55% (P < 0.05). The inclusion of resveratrol was effective in maintaining sperm viability; in particular, it was effective in maintaining plasmatic membrane integrity during the cryopreservation process up to 0.039 mg mL-1, meaning that it could be an alternative to conventionally used seminal extenders in goats. |
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Semina. Ciências Agrárias (Online) |
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Does resveratrol preserve the integrity of the sperm membrane in goats after thawing?O resveratrol preserva a integridade da membrana espermática de caprinos após descongelamento?AntioxidantsOxidative stressSpermTRIS-egg yolk.AntioxidantesEspermatozoideEstresse oxidativoTRIS gema de ovo.This study aimed to determine the effects of resveratrol in the trisaminomethane (TRIS)-egg yolk extender and its optimal inclusion level for goat semen cryopreservation. Five ejaculates of three Anglo Nubian goats were used, each divided into four 200 µL aliquots for use in four treatments: 0.00 (control), 0.04, 0.08 and 0.12 mg mL-1 resveratrol in the TRIS-egg yolk extender. We evaluated progressive sperm motility and sperm vigor post-dilution, post-cooling, and post thawing; membrane integrity (HOST); and acrosomal integrity and performed a slow thermoresistance test (STT). The data were submitted to a regression analysis at a 5% probability. There was no difference in progressive motility or sperm vigor in the post-dilution (89.5, 89.0, 88.7 and 88.3, and 4.9, 5.0, 4.9, and 4.9) or post-cooling (81.0, 82.0, 83.0, and 78.3; and 4.3, 4.3, 4.2, and 4.2) experiments (P > 0.05), or in the complementary acrosomal integrity test (42.0, 47.4, 42.2 and 38.2) (P > 0.05). However, the motility and vigor parameters decreased linearly in the post-thaw phase, as well as during the 2 hours of incubation on STT (P < 0.05). These factors increased quadratically when resveratrol was added to HOST, to an optimal level of 0.039 mg mL-1 resveratrol for a plasma membrane integrity of 52.55% (P < 0.05). The inclusion of resveratrol was effective in maintaining sperm viability; in particular, it was effective in maintaining plasmatic membrane integrity during the cryopreservation process up to 0.039 mg mL-1, meaning that it could be an alternative to conventionally used seminal extenders in goats.O objetivo deste estudo foi determinar os efeitos e o melhor nível de inclusão de resveratrol em meio diluidor TRIS gema de ovo para criopreservação de sêmen caprino. Foram utilizados cinco ejaculados de três bodes da raça Anglo Nubiana. Cada ejaculado foi dividido em quatro alíquotas de 200 µL, compondo quatro tratamentos: 0,00 (controle); 0,04; 0,08 e 0,12mg mL-1 de resveratrol no diluidor TRIS gema de ovo. Foram avaliadas a motilidade espermática progressiva e o vigor espermático pós-diluição, pós-resfriamento e pós-descongelamento; integridade de membrana (HOST); integridade acrossomal e teste de termorresistência lento (TTR). Os dados foram submetidos à análise de regressão a 5% de probabilidade. Não houve diferença para a motilidade progressiva e o vigor espermático, respectivamente, na pós-diluição (89,5; 89,0; 88,7 e 88,3) e (4,9; 5,0; 4,9 e 4,9) e pós-resfriamento (81,0; 82,0; 83,0 e 78,3) e (4,3; 4,3; 4,2 e 4,2) (P < 0,05), assim como para o teste complementar integridade acrossomal (42,0; 47,4; 42,2 e 38,2) (P > 0,05). No entanto, os parâmetros motilidade e vigor reduziram linearmente no pós-descongelamento, assim como durante as 2 horas de incubação no TTR (P < 0,05). Houve efeito quadrático com a inclusão de resveratrol para HOST, apresentando um nível ótimo de 0,039mg mL-1 de resveratrol para integridade de membrana plasmática de 52,55% (P < 0,05). A inclusão de resveratrol foi eficiente na manutenção da viabilidade espermática; em especial, foi eficiente na manutenção da integridade da membrana plasmática durante o processo de criopreservação até o nível de 0,039mg mL-1, podendo ser uma alternativa na composição dos diluidores seminais de caprinos.UEL2020-11-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionTipoTipoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3932610.5433/1679-0359.2020v41n6Supl2p3189Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3189-3198Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3189-31981679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39326/28275Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBrito, Lindomar SousaBarbosa, Larissa PiresPinheiro, Alexandre MoraesResende, Max VitóriaCosta, Nariane Gavazza de LimaMachado, William MoraisJesus, Ronival Dias Lima deRibeiro, Márcio de OliveiraFrança, Caline Santana daCardoso, Reuber de Carvalho2022-10-04T16:47:20Zoai:ojs.pkp.sfu.ca:article/39326Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T16:47:20Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? O resveratrol preserva a integridade da membrana espermática de caprinos após descongelamento? |
title |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? |
spellingShingle |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? Brito, Lindomar Sousa Antioxidants Oxidative stress Sperm TRIS-egg yolk. Antioxidantes Espermatozoide Estresse oxidativo TRIS gema de ovo. |
title_short |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? |
title_full |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? |
title_fullStr |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? |
title_full_unstemmed |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? |
title_sort |
Does resveratrol preserve the integrity of the sperm membrane in goats after thawing? |
author |
Brito, Lindomar Sousa |
author_facet |
Brito, Lindomar Sousa Barbosa, Larissa Pires Pinheiro, Alexandre Moraes Resende, Max Vitória Costa, Nariane Gavazza de Lima Machado, William Morais Jesus, Ronival Dias Lima de Ribeiro, Márcio de Oliveira França, Caline Santana da Cardoso, Reuber de Carvalho |
author_role |
author |
author2 |
Barbosa, Larissa Pires Pinheiro, Alexandre Moraes Resende, Max Vitória Costa, Nariane Gavazza de Lima Machado, William Morais Jesus, Ronival Dias Lima de Ribeiro, Márcio de Oliveira França, Caline Santana da Cardoso, Reuber de Carvalho |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Brito, Lindomar Sousa Barbosa, Larissa Pires Pinheiro, Alexandre Moraes Resende, Max Vitória Costa, Nariane Gavazza de Lima Machado, William Morais Jesus, Ronival Dias Lima de Ribeiro, Márcio de Oliveira França, Caline Santana da Cardoso, Reuber de Carvalho |
dc.subject.por.fl_str_mv |
Antioxidants Oxidative stress Sperm TRIS-egg yolk. Antioxidantes Espermatozoide Estresse oxidativo TRIS gema de ovo. |
topic |
Antioxidants Oxidative stress Sperm TRIS-egg yolk. Antioxidantes Espermatozoide Estresse oxidativo TRIS gema de ovo. |
description |
This study aimed to determine the effects of resveratrol in the trisaminomethane (TRIS)-egg yolk extender and its optimal inclusion level for goat semen cryopreservation. Five ejaculates of three Anglo Nubian goats were used, each divided into four 200 µL aliquots for use in four treatments: 0.00 (control), 0.04, 0.08 and 0.12 mg mL-1 resveratrol in the TRIS-egg yolk extender. We evaluated progressive sperm motility and sperm vigor post-dilution, post-cooling, and post thawing; membrane integrity (HOST); and acrosomal integrity and performed a slow thermoresistance test (STT). The data were submitted to a regression analysis at a 5% probability. There was no difference in progressive motility or sperm vigor in the post-dilution (89.5, 89.0, 88.7 and 88.3, and 4.9, 5.0, 4.9, and 4.9) or post-cooling (81.0, 82.0, 83.0, and 78.3; and 4.3, 4.3, 4.2, and 4.2) experiments (P > 0.05), or in the complementary acrosomal integrity test (42.0, 47.4, 42.2 and 38.2) (P > 0.05). However, the motility and vigor parameters decreased linearly in the post-thaw phase, as well as during the 2 hours of incubation on STT (P < 0.05). These factors increased quadratically when resveratrol was added to HOST, to an optimal level of 0.039 mg mL-1 resveratrol for a plasma membrane integrity of 52.55% (P < 0.05). The inclusion of resveratrol was effective in maintaining sperm viability; in particular, it was effective in maintaining plasmatic membrane integrity during the cryopreservation process up to 0.039 mg mL-1, meaning that it could be an alternative to conventionally used seminal extenders in goats. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Tipo Tipo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39326 10.5433/1679-0359.2020v41n6Supl2p3189 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39326 |
identifier_str_mv |
10.5433/1679-0359.2020v41n6Supl2p3189 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39326/28275 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 6Supl2 (2020); 3189-3198 Semina: Ciências Agrárias; v. 41 n. 6Supl2 (2020); 3189-3198 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306082726707200 |