Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems

Detalhes bibliográficos
Autor(a) principal: Bicalho, Juliana Marques
Data de Publicação: 2016
Outros Autores: Fonseca Júnior, Antônio Augusto, Oliveira, Cairo Henrique Sousa de, Resende, Cláudia Fideles, Kassar, Telissa da Cunha, Galinari, Grazielle Cossenzo Florentino, Oliveira, André Penido, Orzil, Livia de Lima, Reis, Jenner Karlisson Pimenta dos, Leite, Rômulo Cerqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23067
Resumo: Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance.
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spelling Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problemsDetecção molecular do Vírus da Língua Azul (BTV) e do Vírus da Leucemia bovina (BLV) em amostras de biópsia uterina de vacas leiteiras com e sem problemas reprodutivosBovinesReproductionUterusBTVBLV.BovinosReproduçãoÚteroBTVBLV.Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance.O desempenho reprodutivo das vacas leiteiras tem impacto direto na produtividade dos rebanhos. Agentes infecciosos como o Vírus da Língua Azul (BTV) e o Vírus da Leucemia Bovina (BLV) estãoassociados à ocorrência de falhas reprodutivas. Entretanto, não se sabe se estes vírus poderiam estar presentes no útero resultando nas perdas gestacionais. Este estudo utilizou métodos moleculares para investigar se os vírus BTV e BLV estão presentes no úterio de vacas leiteiras sorologicamente positivas com histórico de abortos, natimortos e repetição de cio (n=23) e em vacas sem histórico de problema reprodutivos (n =23) provenientes de três rebanhos leiteiros localizados no estado de Minas Gerais, Brasil. Não foi detectado BTV em nenhuma amostra de biópsia uterina. Por outro lado, o DNA proviral de BLV foi detectado no útero de 54.5 % das vacas soropositivas, independentemente da ocorrência de problemas reprodutivos. Conclui-se que o vírus BLV, ao contrário do BTV está presente no útero de vacas soropositivas, entretanto, sem associação com o desempenho reprodutivo.UEL2016-10-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionField Empirical ResearchPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2306710.5433/1679-0359.2016v37n5p3125Semina: Ciências Agrárias; Vol. 37 No. 5 (2016); 3125-3132Semina: Ciências Agrárias; v. 37 n. 5 (2016); 3125-31321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23067/19765http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBicalho, Juliana MarquesFonseca Júnior, Antônio AugustoOliveira, Cairo Henrique Sousa deResende, Cláudia FidelesKassar, Telissa da CunhaGalinari, Grazielle Cossenzo FlorentinoOliveira, André PenidoOrzil, Livia de LimaReis, Jenner Karlisson Pimenta dosLeite, Rômulo Cerqueira2022-11-30T14:09:56Zoai:ojs.pkp.sfu.ca:article/23067Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-30T14:09:56Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
Detecção molecular do Vírus da Língua Azul (BTV) e do Vírus da Leucemia bovina (BLV) em amostras de biópsia uterina de vacas leiteiras com e sem problemas reprodutivos
title Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
spellingShingle Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
Bicalho, Juliana Marques
Bovines
Reproduction
Uterus
BTV
BLV.
Bovinos
Reprodução
Útero
BTV
BLV.
title_short Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
title_full Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
title_fullStr Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
title_full_unstemmed Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
title_sort Molecular detection of Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV) in uterine biopsies of dairy cows with or without reproductive problems
author Bicalho, Juliana Marques
author_facet Bicalho, Juliana Marques
Fonseca Júnior, Antônio Augusto
Oliveira, Cairo Henrique Sousa de
Resende, Cláudia Fideles
Kassar, Telissa da Cunha
Galinari, Grazielle Cossenzo Florentino
Oliveira, André Penido
Orzil, Livia de Lima
Reis, Jenner Karlisson Pimenta dos
Leite, Rômulo Cerqueira
author_role author
author2 Fonseca Júnior, Antônio Augusto
Oliveira, Cairo Henrique Sousa de
Resende, Cláudia Fideles
Kassar, Telissa da Cunha
Galinari, Grazielle Cossenzo Florentino
Oliveira, André Penido
Orzil, Livia de Lima
Reis, Jenner Karlisson Pimenta dos
Leite, Rômulo Cerqueira
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bicalho, Juliana Marques
Fonseca Júnior, Antônio Augusto
Oliveira, Cairo Henrique Sousa de
Resende, Cláudia Fideles
Kassar, Telissa da Cunha
Galinari, Grazielle Cossenzo Florentino
Oliveira, André Penido
Orzil, Livia de Lima
Reis, Jenner Karlisson Pimenta dos
Leite, Rômulo Cerqueira
dc.subject.por.fl_str_mv Bovines
Reproduction
Uterus
BTV
BLV.
Bovinos
Reprodução
Útero
BTV
BLV.
topic Bovines
Reproduction
Uterus
BTV
BLV.
Bovinos
Reprodução
Útero
BTV
BLV.
description Reproductive performance of dairy cows has a direct impact on herd productivity. Infectious agents, such as Bluetongue Virus (BTV) and Bovine Leukemia Virus (BLV), are associated with reproductive failure. However, it remains unknown if these viruses are present in the uterus and cause gestational loss. This study used molecular methods to assess if BTV and BLV can be detected in the uterus of serologically positive dairy cows with a record of abortions, stillbirths and repeat breeding (n=23) and without a record of reproductive problems (n =23). The cows came from three dairy herds of the state of Minas Gerais, Brazil. BTV was not detected in any of the uterine biopsies. Proviral DNA of BLV was detected in 54.5 % of the seropositive cows, but positivity for BLV in the uterus was not associated with the existence of reproductive problems. In conclusion, this study shows that BLV, but not BTV, is present in the uterus of seropositive cows, regardless of reproductive performance.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Field Empirical Research
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23067
10.5433/1679-0359.2016v37n5p3125
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23067
identifier_str_mv 10.5433/1679-0359.2016v37n5p3125
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23067/19765
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 37 No. 5 (2016); 3125-3132
Semina: Ciências Agrárias; v. 37 n. 5 (2016); 3125-3132
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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