Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35383 |
Resumo: | There are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg?1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL?1 in total aerobes and 7 × 101 CFU mL?1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL?1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection. |
id |
UEL-11_7ff03ee744400de6964fc2d015545c51 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/35383 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solutionAnálise microbiológica na fixação e conservação de cadáveres de cães com álcool etílico e solução de cloreto de sódioAnatomyAnimalBacteriumSurgeryMicrobiological counting.AnatomiaAnimalBactériaCirurgiaContagem microbiológica.There are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg?1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL?1 in total aerobes and 7 × 101 CFU mL?1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL?1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection.Várias são as soluções fixadoras ou conservantes de cadáveres, e o formaldeído é o mais utilizado. Entretanto, esse agente pode apresentar efeitos negativos para os profissionais que o manipulam. Portanto, o objetivo deste trabalho foi identificar e quantificar os principais microrganismos relacionados a fixação e conservação de cadáveres de cães utilizando-se solução alternativa e livres de formaldeído. Após injeção arterial (120 mL kg?1, de 95% de álcool etílico 96° GL e 5% de glicerina pura), foram colocados em álcool etílico 96° GL por 30 dias (grupo 1), 60 dias (grupo 2), 90 dias (grupo 3) e 120 dias (grupo 4). Após o período de fixação, permaneceram sob conservação em solução aquosa de cloreto de sódio 30% por 120 dias. A quantificação bacteriana foi realizada por plaqueamento em profundidade (Pour Plate). Durante a fixação, houve presença de população bacteriana em todos os grupos, exceto no grupo 1, e nunca foi ultrapassado o valor de 9 × 101 UFC mL?1 (Unidade Formadora de Colônia) nos aeróbios totais e 7 × 101 UFC mL?1 nos anaeróbios totais. Durante a conservação, houve presença de população microbiana em todos os grupos em pelo menos dois momentos, e nunca foi ultrapassado o valor de 7 × 101 UFC mL?1 nos aeróbios totais e nos anaeróbios totais. A presença de fungos foi observada em 8 das 34 análises. Houve identificação de Pseudomonas sp., E. coli e Bacillus sp. nas amostras analisadas. A contagem microbiológica foi baixa e não foram observados, à inspeção visual, sinais de contaminação nas cubas.UEL2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3538310.5433/1679-0359.2019v40n6Supl2p3099Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3099-3106Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3099-31061679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35383/26134Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPereira, NatáliaCardozo, Marita VedovelliRocha, Thiago André Salvitti de SáZero, Raphael ChiareloÁvila, Fernando Antônio deOliveira, Fabrício Singaretti de2022-10-10T16:12:44Zoai:ojs.pkp.sfu.ca:article/35383Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:12:44Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution Análise microbiológica na fixação e conservação de cadáveres de cães com álcool etílico e solução de cloreto de sódio |
title |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
spellingShingle |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution Pereira, Natália Anatomy Animal Bacterium Surgery Microbiological counting. Anatomia Animal Bactéria Cirurgia Contagem microbiológica. |
title_short |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_full |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_fullStr |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_full_unstemmed |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
title_sort |
Microbiological analysis in the fixation and preservation of dog cadavers with ethyl alcohol and sodium chloride solution |
author |
Pereira, Natália |
author_facet |
Pereira, Natália Cardozo, Marita Vedovelli Rocha, Thiago André Salvitti de Sá Zero, Raphael Chiarelo Ávila, Fernando Antônio de Oliveira, Fabrício Singaretti de |
author_role |
author |
author2 |
Cardozo, Marita Vedovelli Rocha, Thiago André Salvitti de Sá Zero, Raphael Chiarelo Ávila, Fernando Antônio de Oliveira, Fabrício Singaretti de |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pereira, Natália Cardozo, Marita Vedovelli Rocha, Thiago André Salvitti de Sá Zero, Raphael Chiarelo Ávila, Fernando Antônio de Oliveira, Fabrício Singaretti de |
dc.subject.por.fl_str_mv |
Anatomy Animal Bacterium Surgery Microbiological counting. Anatomia Animal Bactéria Cirurgia Contagem microbiológica. |
topic |
Anatomy Animal Bacterium Surgery Microbiological counting. Anatomia Animal Bactéria Cirurgia Contagem microbiológica. |
description |
There are several fixative or preservative solutions for use on cadavers, and formaldehyde is the most widely used. However, this chemical may present negative effects for professionals who manipulate it. Therefore, this study aimed to identify and quantify the main microorganisms related to the fixation and preservation of dog cadavers using an alternative and formaldehyde-free solution. After arterial injection (120 mL kg?1 95% 96° GL ethyl alcohol and 5% pure glycerin), cadavers were placed in 96° GL ethyl alcohol for 30 (group 1), 60 (group 2), 90 (group 3), and 120 days (group 4). After the fixation period, they remained under preservation in a 30% aqueous sodium chloride solution for 120 days. Bacterial quantification was performed by the pour plate method. The bacterial population was present in all groups during fixation, except for group 1, but never exceeded 9 × 101 CFU mL?1 in total aerobes and 7 × 101 CFU mL?1 in total anaerobes. The microbial population was present in all groups in at least two moments during preservation and never exceeded 7 × 101 CFU mL?1 in total aerobes and anaerobes. The presence of fungi was observed in 8 out of 34 analyses. Pseudomonas sp., Escherichia coli, and Bacillus sp. were identified in the analyzed samples. Microbiological counting was low, and no signs of contamination were observed in the vats at visual inspection. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35383 10.5433/1679-0359.2019v40n6Supl2p3099 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35383 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6Supl2p3099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/35383/26134 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3099-3106 Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3099-3106 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306081054228480 |