Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.

Detalhes bibliográficos
Autor(a) principal: Monteiro, Jessica Nascimento Moraes
Data de Publicação: 2017
Outros Autores: Archanjo, Anderson Barros, Passos, Gabriela Porfirio, Costa, Adilson Vidal, Porfirio, Lenir Cardoso, Martins, Isabella Vilhena Freire
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470
Resumo: The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces.
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spelling Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.Extrato etanólico e óleo essencial de Chenopodium ambrosioides L. no controle de Ancylostoma spp. de cãesEssential oilHelminthsHerb of Santa MariaMedicinal plants.Erva-de-santa-mariaHelmintosÓleo essencialPlanta medicinal.The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces.O uso de Chenopodium ambrosioides L. tem se mostrado promissor no controle de nematoides gastrintestinais. Objetivou-se verificar o rendimento e a composição química do óleo essencial de C. ambrosioides, assim como avaliar o efeito in vitro do extrato etanólico e do óleo essencial em L3 de Ancylostoma spp., e o efeito in vivo do óleo essencial para cães. Para a atividade larvicida as larvas de Ancylostoma spp. foram colocadas na presença do extrato etanólico nas concentrações de 0,005 g mL-1, 0,01 g mL-1, 0,02 g mL-1, 0,03 g mL-1, 0,04 g mL-1; 0,05 g mL-1, 0,06 g mL-1, 0,08 g mL-1, 0,1 g mL-1, 0,12 g mL-1 e 0,2 g mL-1, do óleo essencial nas concentrações de 50 µL mL-1, 100 µL mL-1 e 150 µL mL-1. Para o teste in vivo utilizaram-se 26 cães, hígidos, infectados naturalmente por Ancylostoma spp., divididos em três grupos: F1 - foram administrados biscoitos sem princípio ativo; F2 - biscoitos fitoterápicos com óleo essencial de C. ambrosioides L na concentração de 37,5 µL g-1; F3 biscoitos acrescido de formulação comercial a base de febantel, pirantel, praziquantel e ivermectina. Foi realizado hemograma completo e bioquímica sérica para AST, ALT, FA, ureia, creatinina, proteína total e albumina. Verificou-se o rendimento de 0,3% m v-1 do óleo essencial e na sua composição química a presença de ?-terpineno (1,24 %), o p-cimeno (4,83 %) e ascaridol, nos seus isômeros Z (87%) e E (5,04 %), como componente majoritário. O extrato etanólico de C. ambrosioides L., nas concentrações utilizadas, apresentou insuficiente atividade para o efeito larvicida. O óleo essencial na concentração de 150 µL mL-1 foi eficaz contra L3. A formulação de biscoito fitoterápico com óleo essencial C. ambrosioides L reduziu o número de ovos por grama de fezes dos cães.UEL2017-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisapesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2647010.5433/1679-0359.2017v38n4p1947Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1947-1954Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1947-19541679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470/21372Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMonteiro, Jessica Nascimento MoraesArchanjo, Anderson BarrosPassos, Gabriela PorfirioCosta, Adilson VidalPorfirio, Lenir CardosoMartins, Isabella Vilhena Freire2022-10-24T12:31:52Zoai:ojs.pkp.sfu.ca:article/26470Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T12:31:52Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
Extrato etanólico e óleo essencial de Chenopodium ambrosioides L. no controle de Ancylostoma spp. de cães
title Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
spellingShingle Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
Monteiro, Jessica Nascimento Moraes
Essential oil
Helminths
Herb of Santa Maria
Medicinal plants.
Erva-de-santa-maria
Helmintos
Óleo essencial
Planta medicinal.
title_short Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
title_full Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
title_fullStr Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
title_full_unstemmed Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
title_sort Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
author Monteiro, Jessica Nascimento Moraes
author_facet Monteiro, Jessica Nascimento Moraes
Archanjo, Anderson Barros
Passos, Gabriela Porfirio
Costa, Adilson Vidal
Porfirio, Lenir Cardoso
Martins, Isabella Vilhena Freire
author_role author
author2 Archanjo, Anderson Barros
Passos, Gabriela Porfirio
Costa, Adilson Vidal
Porfirio, Lenir Cardoso
Martins, Isabella Vilhena Freire
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Monteiro, Jessica Nascimento Moraes
Archanjo, Anderson Barros
Passos, Gabriela Porfirio
Costa, Adilson Vidal
Porfirio, Lenir Cardoso
Martins, Isabella Vilhena Freire
dc.subject.por.fl_str_mv Essential oil
Helminths
Herb of Santa Maria
Medicinal plants.
Erva-de-santa-maria
Helmintos
Óleo essencial
Planta medicinal.
topic Essential oil
Helminths
Herb of Santa Maria
Medicinal plants.
Erva-de-santa-maria
Helmintos
Óleo essencial
Planta medicinal.
description The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces.
publishDate 2017
dc.date.none.fl_str_mv 2017-08-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470
10.5433/1679-0359.2017v38n4p1947
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470
identifier_str_mv 10.5433/1679-0359.2017v38n4p1947
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470/21372
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1947-1954
Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1947-1954
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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