Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470 |
Resumo: | The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces. |
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Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp.Extrato etanólico e óleo essencial de Chenopodium ambrosioides L. no controle de Ancylostoma spp. de cãesEssential oilHelminthsHerb of Santa MariaMedicinal plants.Erva-de-santa-mariaHelmintosÓleo essencialPlanta medicinal.The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces.O uso de Chenopodium ambrosioides L. tem se mostrado promissor no controle de nematoides gastrintestinais. Objetivou-se verificar o rendimento e a composição química do óleo essencial de C. ambrosioides, assim como avaliar o efeito in vitro do extrato etanólico e do óleo essencial em L3 de Ancylostoma spp., e o efeito in vivo do óleo essencial para cães. Para a atividade larvicida as larvas de Ancylostoma spp. foram colocadas na presença do extrato etanólico nas concentrações de 0,005 g mL-1, 0,01 g mL-1, 0,02 g mL-1, 0,03 g mL-1, 0,04 g mL-1; 0,05 g mL-1, 0,06 g mL-1, 0,08 g mL-1, 0,1 g mL-1, 0,12 g mL-1 e 0,2 g mL-1, do óleo essencial nas concentrações de 50 µL mL-1, 100 µL mL-1 e 150 µL mL-1. Para o teste in vivo utilizaram-se 26 cães, hígidos, infectados naturalmente por Ancylostoma spp., divididos em três grupos: F1 - foram administrados biscoitos sem princípio ativo; F2 - biscoitos fitoterápicos com óleo essencial de C. ambrosioides L na concentração de 37,5 µL g-1; F3 biscoitos acrescido de formulação comercial a base de febantel, pirantel, praziquantel e ivermectina. Foi realizado hemograma completo e bioquímica sérica para AST, ALT, FA, ureia, creatinina, proteína total e albumina. Verificou-se o rendimento de 0,3% m v-1 do óleo essencial e na sua composição química a presença de ?-terpineno (1,24 %), o p-cimeno (4,83 %) e ascaridol, nos seus isômeros Z (87%) e E (5,04 %), como componente majoritário. O extrato etanólico de C. ambrosioides L., nas concentrações utilizadas, apresentou insuficiente atividade para o efeito larvicida. O óleo essencial na concentração de 150 µL mL-1 foi eficaz contra L3. A formulação de biscoito fitoterápico com óleo essencial C. ambrosioides L reduziu o número de ovos por grama de fezes dos cães.UEL2017-08-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpesquisapesquisaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2647010.5433/1679-0359.2017v38n4p1947Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1947-1954Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1947-19541679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470/21372Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMonteiro, Jessica Nascimento MoraesArchanjo, Anderson BarrosPassos, Gabriela PorfirioCosta, Adilson VidalPorfirio, Lenir CardosoMartins, Isabella Vilhena Freire2022-10-24T12:31:52Zoai:ojs.pkp.sfu.ca:article/26470Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-24T12:31:52Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. Extrato etanólico e óleo essencial de Chenopodium ambrosioides L. no controle de Ancylostoma spp. de cães |
title |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
spellingShingle |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. Monteiro, Jessica Nascimento Moraes Essential oil Helminths Herb of Santa Maria Medicinal plants. Erva-de-santa-maria Helmintos Óleo essencial Planta medicinal. |
title_short |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
title_full |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
title_fullStr |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
title_full_unstemmed |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
title_sort |
Chenopodium ambrosioides L. essential oil and ethanol extract on control of canine Ancylostoma spp. |
author |
Monteiro, Jessica Nascimento Moraes |
author_facet |
Monteiro, Jessica Nascimento Moraes Archanjo, Anderson Barros Passos, Gabriela Porfirio Costa, Adilson Vidal Porfirio, Lenir Cardoso Martins, Isabella Vilhena Freire |
author_role |
author |
author2 |
Archanjo, Anderson Barros Passos, Gabriela Porfirio Costa, Adilson Vidal Porfirio, Lenir Cardoso Martins, Isabella Vilhena Freire |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Monteiro, Jessica Nascimento Moraes Archanjo, Anderson Barros Passos, Gabriela Porfirio Costa, Adilson Vidal Porfirio, Lenir Cardoso Martins, Isabella Vilhena Freire |
dc.subject.por.fl_str_mv |
Essential oil Helminths Herb of Santa Maria Medicinal plants. Erva-de-santa-maria Helmintos Óleo essencial Planta medicinal. |
topic |
Essential oil Helminths Herb of Santa Maria Medicinal plants. Erva-de-santa-maria Helmintos Óleo essencial Planta medicinal. |
description |
The use of Chenopodium ambrosioides L. has shown to be promising in the management of gastrointestinal nematodes. The objective of this study was to quantitate the yield and characterize the chemical composition of the essential oil of C. ambrosioides, as well as to evaluate the in vitro effect of the ethanolic extract and the essential oil in L3 of Ancylostoma spp. and the in vivo effect(s) of the essential oil in dogs. The effects of the ethanol extract and essential oil on Ancylostoma spp. were evaluated in vitro by exposing larvae to the extract at concentrations ranging from 0.005 g mL-1 to 0.2 g mL-1 and to essential oil at concentrations of 50, 100, and 150 ?L mL-1. For the in vivo test, 26 healthy dogs, naturally infected by Ancylostoma spp., were divided into three groups: F1 - cookies were administered without active principle; F2 - herbal cookies containing 37.5 ?L g-1 essential oil of C. ambrosioides L.; F3 - cookies plus a commercial formulation containing febantel, pyrantel, praziquantel, and ivermectin. Complete blood counts and serum biochemistry for AST, ALT, AF, urea, creatinine, total protein, and albumin were performed. The yield of the essential oil was 0.3% m v-1, and its major components included ?-terpinene (1.24%), p-cymene (4.83%), and ascaridol Z (87%) and E (5.04%) isomers. The concentrations of C. ambrosioides L. ethanol extract used were ineffective against Ancylostoma spp. larvae. The essential oil at a concentration of 150 ?L mL-1 was effective against L3 larvae. In the in vivo study in dogs, the herbal cookies containing C. ambrosioides L essential oil reduced the number of eggs per gram of feces. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion pesquisa pesquisa |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470 10.5433/1679-0359.2017v38n4p1947 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470 |
identifier_str_mv |
10.5433/1679-0359.2017v38n4p1947 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/26470/21372 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 4 (2017); 1947-1954 Semina: Ciências Agrárias; v. 38 n. 4 (2017); 1947-1954 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306076420571136 |