Development of a qPCR to diagnose the genus Eimeria in bovines

Detalhes bibliográficos
Autor(a) principal: Cardim, Sérgio Tosi
Data de Publicação: 2020
Outros Autores: Cardim, Stefani Lino, Seixas, Mércia de, Taroda, Alessandra, Carneiro, Priscilla Gomes, Zulpo, Dauton Luiz, Barros, Luiz Daniel de, Bortolloti, Guilherme Luis, Garcia, João Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433
Resumo: Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry).
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spelling Development of a qPCR to diagnose the genus Eimeria in bovinesDesenvolvimento de uma qPCR para o diagnóstico do Gênero Eimeria em bovinosqPCREimeriacoccidiosis18S rRNAcattleqPCREimeriaCoccidiose18S rRNABovinos.Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry).A coccidiose em bovinos é causada por protozoários do gênero Eimeria. Estes protozoários acometem principalmente animais jovens, causando diminuição de produção e consequente perdas econômicas. O diagnóstico de rotina é realizado através de observação morfológica dos oocistos, que possui várias limitações. O objetivo do presente trabalho foi desenvolver uma técnica de qPCR para diagnóstico de Eimeria spp. em bovinos. Para isto foi selecionada a região 18S rRNA do DNA destes parasitas, pois a mesma é uma região com pouca variabilidade entre as espécies. A qPCR foi desenvolvida com a utilização de SYBR Green, tendo como resultado uma PCR com uma alta sensibilidade, capaz de amplificar amostras contendo apenas um oocisto de Eimeria spp de bovinos. Demonstra-se nesse estudo a viabilidade na utilização da qPCR no diagnóstico do gênero Eimeria, sendo esta técnica menos laboriosa e com menos necessidade de treinamento para diagnóstico quando comparada com a técnica convencionalmente utilizada em rotina (micromorfometria).UEL2020-09-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3843310.5433/1679-0359.2020v41n6p2695Semina: Ciências Agrárias; Vol. 41 No. 6 (2020); 2695-2702Semina: Ciências Agrárias; v. 41 n. 6 (2020); 2695-27021679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433/28071Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCardim, Sérgio TosiCardim, Stefani LinoSeixas, Mércia deTaroda, AlessandraCarneiro, Priscilla GomesZulpo, Dauton LuizBarros, Luiz Daniel deBortolloti, Guilherme LuisGarcia, João Luis2022-10-07T11:42:56Zoai:ojs.pkp.sfu.ca:article/38433Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T11:42:56Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Development of a qPCR to diagnose the genus Eimeria in bovines
Desenvolvimento de uma qPCR para o diagnóstico do Gênero Eimeria em bovinos
title Development of a qPCR to diagnose the genus Eimeria in bovines
spellingShingle Development of a qPCR to diagnose the genus Eimeria in bovines
Cardim, Sérgio Tosi
qPCR
Eimeria
coccidiosis
18S rRNA
cattle
qPCR
Eimeria
Coccidiose
18S rRNA
Bovinos.
title_short Development of a qPCR to diagnose the genus Eimeria in bovines
title_full Development of a qPCR to diagnose the genus Eimeria in bovines
title_fullStr Development of a qPCR to diagnose the genus Eimeria in bovines
title_full_unstemmed Development of a qPCR to diagnose the genus Eimeria in bovines
title_sort Development of a qPCR to diagnose the genus Eimeria in bovines
author Cardim, Sérgio Tosi
author_facet Cardim, Sérgio Tosi
Cardim, Stefani Lino
Seixas, Mércia de
Taroda, Alessandra
Carneiro, Priscilla Gomes
Zulpo, Dauton Luiz
Barros, Luiz Daniel de
Bortolloti, Guilherme Luis
Garcia, João Luis
author_role author
author2 Cardim, Stefani Lino
Seixas, Mércia de
Taroda, Alessandra
Carneiro, Priscilla Gomes
Zulpo, Dauton Luiz
Barros, Luiz Daniel de
Bortolloti, Guilherme Luis
Garcia, João Luis
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cardim, Sérgio Tosi
Cardim, Stefani Lino
Seixas, Mércia de
Taroda, Alessandra
Carneiro, Priscilla Gomes
Zulpo, Dauton Luiz
Barros, Luiz Daniel de
Bortolloti, Guilherme Luis
Garcia, João Luis
dc.subject.por.fl_str_mv qPCR
Eimeria
coccidiosis
18S rRNA
cattle
qPCR
Eimeria
Coccidiose
18S rRNA
Bovinos.
topic qPCR
Eimeria
coccidiosis
18S rRNA
cattle
qPCR
Eimeria
Coccidiose
18S rRNA
Bovinos.
description Bovine coccidiosis is caused by protozoa of the genus Eimeria. These protozoa mainly affect young animals, causing a decrease in production and consequent economic losses. The routine diagnosis is made through morphological observation of the oocysts, which has several limitations. The objective of the present study was to develop a qPCR technique for the diagnose of Eimeria spp. in cattle. For this purpose, the 18S rRNA region of the DNA of these parasites was selected, since it is a region with low variability among the species. The qPCR was developed using the SYBR Green, resulting in a PCR with a high sensitivity, able to amplify samples containing only one oocyst of Eimeria spp. of bovines. The feasibility of using qPCR in the diagnosis of the Eimeria Genus is demonstrated in this study, once this technique shows to be less laborious and needs less skills for diagnostic training when compared to the technique conventionally used in theroutine (micromorphometry).
publishDate 2020
dc.date.none.fl_str_mv 2020-09-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433
10.5433/1679-0359.2020v41n6p2695
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433
identifier_str_mv 10.5433/1679-0359.2020v41n6p2695
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38433/28071
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 6 (2020); 2695-2702
Semina: Ciências Agrárias; v. 41 n. 6 (2020); 2695-2702
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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