Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11516 |
Resumo: | This study aimed to determine the antimicrobial susceptibility profile and to assess the presence of mechanisms of antimicrobial resistance in Staphylococcus spp. (n=36) isolated from mastitis in sheep’s Chapecó-SC. The potential for biofilm production was determined by phenotypic tests of Congo Red Agar, DAPI and Gentian Violet and by PCR for the detection of icaD gene. To evaluate the antimicrobial resistance testing was performed disk diffusion and detection of resistance genes blaZ, mecA, ermA, ermB and ermC and msrA also performed by PCR.The pump test was conducted by efuxo crop growth Muller Hinton agar containing ethidium bromide. The results showed that 1 (2,78%), 36 (100%) and 10 (27,78%) isolates were considered to produce a biofilm on Congo Red Agar test, Gentian Violet and DAPI, respectively, while the gene icaD was observed in only 2 (5.55%) isolates. The lowest percentage of sensitivity was observed for ampicillin (58.33%) and penicillin (58.33%). All strains tested were negative for the mecA, ermA, ermB and ermC genes. However, the isolates were positive for other resistance genes, being the blaZ and the msrA, with percentages of positivity of 58.33% and 11.11% respectively. Only one sample was positive for efflux pump test. |
id |
UEL-11_96041b6ff742fc5d73bc6b11e831e1b6 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/11516 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SCCaracterização fenotípica e molecular de isolados de Staphylococcus spp. obtidos de leite de ovelhas do Município de Chapecó-SCMastitissheepResistance genesEfflux pumpBiofilmPCR.MastiteOvinosGenes de resistênciaBomba de efluxoBiofilmePCR.microbiologia veterináriaThis study aimed to determine the antimicrobial susceptibility profile and to assess the presence of mechanisms of antimicrobial resistance in Staphylococcus spp. (n=36) isolated from mastitis in sheep’s Chapecó-SC. The potential for biofilm production was determined by phenotypic tests of Congo Red Agar, DAPI and Gentian Violet and by PCR for the detection of icaD gene. To evaluate the antimicrobial resistance testing was performed disk diffusion and detection of resistance genes blaZ, mecA, ermA, ermB and ermC and msrA also performed by PCR.The pump test was conducted by efuxo crop growth Muller Hinton agar containing ethidium bromide. The results showed that 1 (2,78%), 36 (100%) and 10 (27,78%) isolates were considered to produce a biofilm on Congo Red Agar test, Gentian Violet and DAPI, respectively, while the gene icaD was observed in only 2 (5.55%) isolates. The lowest percentage of sensitivity was observed for ampicillin (58.33%) and penicillin (58.33%). All strains tested were negative for the mecA, ermA, ermB and ermC genes. However, the isolates were positive for other resistance genes, being the blaZ and the msrA, with percentages of positivity of 58.33% and 11.11% respectively. Only one sample was positive for efflux pump test.O presente trabalho teve como objetivos determinar o perfil de sensibilidade aos antimicrobianos e avaliar a presença de mecanismos de resistência antimicrobiana em Staphylococcus spp. (n=36) isolados de mastite em ovelhas do município de Chapecó-SC. O potencial para produção de biofilme foi determinado pelos testes fenotípicos de Agar Vermelho Congo, DAPI e Violeta de Genciana e por teste molecular pela técnica de PCR para a detecção do gene icaD. Para determinar o perfil de resistência aos antimicrobianos, foi realizado o teste de difusão em disco e detecção dos genes de resistência blaZ, mecA, erm (A, B e C) e msrA. O teste da bomba de efuxo foi realizado através do crescimento das culturas em Agar Muller Hinton contendo brometo de etídio. Os resultados mostraram que 1 (2,78%), 36 (100%) e 10 (27,78%) isolados foram considerados produtores de biofilme pelos testes de Agar Vermelho Congo, DAPI e Violeta de Genciana, respectivamente, enquanto que o gene icaD foi observado em apenas 2 (5,55%) isolados. O menor percentual de sensibilidade foi observado para ampicilina (58,33%) e penicilina (58,33%). Os isolados avaliados foram positivos para os genes de resistência blaZ (58,33%) e msrA (11,11%). Nenhum isolado apresentou os genes de resistência erm (A, B, C) e apenas uma amostra foi positiva para o teste da bomba de efluxo. UEL2013-03-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1151610.5433/1679-0359.2013v34n1p311Semina: Ciências Agrárias; Vol. 34 No. 1 (2013); 311-322Semina: Ciências Agrárias; v. 34 n. 1 (2013); 311-3221679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11516/pdfCopyright (c) 2013 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessDall Agnol, Alais MariaCavalcante, Marielly BastosFrança, Chirles Araújo deKrewer, Carina da CostaQueiros, Anderson Antônio deCosta, Mateus Matiuzzi daBragança, José Francisco MantaGirardini, Lilian Kolling2023-01-26T14:40:27Zoai:ojs.pkp.sfu.ca:article/11516Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-26T14:40:27Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC Caracterização fenotípica e molecular de isolados de Staphylococcus spp. obtidos de leite de ovelhas do Município de Chapecó-SC |
title |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC |
spellingShingle |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC Dall Agnol, Alais Maria Mastitis sheep Resistance genes Efflux pump Biofilm PCR. Mastite Ovinos Genes de resistência Bomba de efluxo Biofilme PCR. microbiologia veterinária |
title_short |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC |
title_full |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC |
title_fullStr |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC |
title_full_unstemmed |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC |
title_sort |
Phenotypic and molecular characterization of isolates of Staphylococcus spp. obtained from sheep milk Chapecó-SC |
author |
Dall Agnol, Alais Maria |
author_facet |
Dall Agnol, Alais Maria Cavalcante, Marielly Bastos França, Chirles Araújo de Krewer, Carina da Costa Queiros, Anderson Antônio de Costa, Mateus Matiuzzi da Bragança, José Francisco Manta Girardini, Lilian Kolling |
author_role |
author |
author2 |
Cavalcante, Marielly Bastos França, Chirles Araújo de Krewer, Carina da Costa Queiros, Anderson Antônio de Costa, Mateus Matiuzzi da Bragança, José Francisco Manta Girardini, Lilian Kolling |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Dall Agnol, Alais Maria Cavalcante, Marielly Bastos França, Chirles Araújo de Krewer, Carina da Costa Queiros, Anderson Antônio de Costa, Mateus Matiuzzi da Bragança, José Francisco Manta Girardini, Lilian Kolling |
dc.subject.por.fl_str_mv |
Mastitis sheep Resistance genes Efflux pump Biofilm PCR. Mastite Ovinos Genes de resistência Bomba de efluxo Biofilme PCR. microbiologia veterinária |
topic |
Mastitis sheep Resistance genes Efflux pump Biofilm PCR. Mastite Ovinos Genes de resistência Bomba de efluxo Biofilme PCR. microbiologia veterinária |
description |
This study aimed to determine the antimicrobial susceptibility profile and to assess the presence of mechanisms of antimicrobial resistance in Staphylococcus spp. (n=36) isolated from mastitis in sheep’s Chapecó-SC. The potential for biofilm production was determined by phenotypic tests of Congo Red Agar, DAPI and Gentian Violet and by PCR for the detection of icaD gene. To evaluate the antimicrobial resistance testing was performed disk diffusion and detection of resistance genes blaZ, mecA, ermA, ermB and ermC and msrA also performed by PCR.The pump test was conducted by efuxo crop growth Muller Hinton agar containing ethidium bromide. The results showed that 1 (2,78%), 36 (100%) and 10 (27,78%) isolates were considered to produce a biofilm on Congo Red Agar test, Gentian Violet and DAPI, respectively, while the gene icaD was observed in only 2 (5.55%) isolates. The lowest percentage of sensitivity was observed for ampicillin (58.33%) and penicillin (58.33%). All strains tested were negative for the mecA, ermA, ermB and ermC genes. However, the isolates were positive for other resistance genes, being the blaZ and the msrA, with percentages of positivity of 58.33% and 11.11% respectively. Only one sample was positive for efflux pump test. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11516 10.5433/1679-0359.2013v34n1p311 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11516 |
identifier_str_mv |
10.5433/1679-0359.2013v34n1p311 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11516/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 34 No. 1 (2013); 311-322 Semina: Ciências Agrárias; v. 34 n. 1 (2013); 311-322 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306066667765760 |