Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State

Detalhes bibliográficos
Autor(a) principal: Spiti, Simone Marins
Data de Publicação: 2018
Outros Autores: Barros, Luiz Daniel de, Seixas, Mércia de, Minutti, Ana Flavia, Martins, Thais Agostinho, Cunha Filho, Luiz Fernando Coelho da, Cardim, Sergio Tosi, Kono, Isabelli Sayuri, Nino, Beatriz de Souza Lima, Bogado, Alexey Leon Gomel, Garcia, João Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
DOI: 10.5433/1679-0359.2018v39n6p2449
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31812
Resumo: Neosporosis is one of the principal causes of reproductive problems in cattle worldwide. The aim of this study was to evaluate the occurrence of anti-Neospora caninum antibodies in cattle and dogs from dairy farms from the central northern region of Paraná state. Blood samples with and without EDTA were collected from 400 cattle and 46 dogs from 20 properties. Nested polymerase chain reaction (n-PCR) was performed on whole blood samples and indirect immunofluorescence reaction (IFR) on the serum samples (after clot retraction). Cattle and dogs with titers ? 100 and ? 50, respectively, were considered positive. Anti-N. caninum was detected in 20,1% (80/400) of cattle, with titers ranging from 100 to 1600. The n-PCR presented only two cattle positives (0.5%). Anti-N. caninum was detected in 19,6% (9/46) of the dogs, with titers ranging from 50 to 6400. The occurrence of antibodies against N. caninum obtained in the present study was similar to those in studies performed in other regions of Paraná and Brazil. The probability of detecting parasitemia in epidemiological studies is a rare event.
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spelling Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná StateOcorrência de anticorpos contra Neospora caninum em bovinos e cães oriundo de propriedades produtoras de leite do Norte Central do Estado do ParanáNeosporosisNeospora caninumReproductive ProblemsDairy cattleSeropositiveAbortion.NeosporoseProblemas reprodutivosSoropositivoAborto.Neosporosis is one of the principal causes of reproductive problems in cattle worldwide. The aim of this study was to evaluate the occurrence of anti-Neospora caninum antibodies in cattle and dogs from dairy farms from the central northern region of Paraná state. Blood samples with and without EDTA were collected from 400 cattle and 46 dogs from 20 properties. Nested polymerase chain reaction (n-PCR) was performed on whole blood samples and indirect immunofluorescence reaction (IFR) on the serum samples (after clot retraction). Cattle and dogs with titers ? 100 and ? 50, respectively, were considered positive. Anti-N. caninum was detected in 20,1% (80/400) of cattle, with titers ranging from 100 to 1600. The n-PCR presented only two cattle positives (0.5%). Anti-N. caninum was detected in 19,6% (9/46) of the dogs, with titers ranging from 50 to 6400. The occurrence of antibodies against N. caninum obtained in the present study was similar to those in studies performed in other regions of Paraná and Brazil. The probability of detecting parasitemia in epidemiological studies is a rare event.A neosporose é uma das principais causas de abortamentos em bovinos no mundo todo. O objetivo do presente estudo foi avaliar a ocorrência de anticorpos contra Neospora caninum em soros de bovinos de leite e cães provenientes de propriedades da região centro norte do estado do Paraná. Sangue com e sem EDTA foram colhidos de 400 bovinos e de 46 cães provenientes de 20 propriedades. Para detecção de anticorpos contra N. caninum a Reação de Imunofluorescência Indireta (RIFI) foi realizada, e bovinos e cães foram considerados positivos quando apresentaram títulos ? 100 e ? 50, respectivamente. Anticorpos contra-N. caninum foram detectados em 20,1% (80/400) dos bovinos, cujos títulos variaram de 100 a 1600. A n-PCR apresentou apenas dois animais positivos (0,5%) para os bovinos. Dos 46 cães estudados 19,6% (9/46) foram considerados positivos na RIFI, com títulos variando de 50 a 6400. Os resultados permitem concluir que a ocorrência de anticorpos contra N. caninum em bovinos e cães na mesorregião centro norte do Paraná foram semelhantes aos observados em outras mesorregiões do Paraná. A n-PCR de sangue total revelou uma baixa positividade em bovinos e cães, mostrando que a probabilidade de se detectar parasitemia em estudos epidemiológicos é baixa.UEL2018-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa científicaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3181210.5433/1679-0359.2018v39n6p2449Semina: Ciências Agrárias; Vol. 39 No. 6 (2018); 2449-2456Semina: Ciências Agrárias; v. 39 n. 6 (2018); 2449-24561679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31812/24636Copyright (c) 2018 Semina: Ciências Agráriasinfo:eu-repo/semantics/openAccessSpiti, Simone MarinsBarros, Luiz Daniel deSeixas, Mércia deMinutti, Ana FlaviaMartins, Thais AgostinhoCunha Filho, Luiz Fernando Coelho daCardim, Sergio TosiKono, Isabelli SayuriNino, Beatriz de Souza LimaBogado, Alexey Leon GomelGarcia, João Luis2019-07-03T16:48:48Zoai:ojs.pkp.sfu.ca:article/31812Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2019-07-03T16:48:48Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
Ocorrência de anticorpos contra Neospora caninum em bovinos e cães oriundo de propriedades produtoras de leite do Norte Central do Estado do Paraná
title Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
spellingShingle Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
Spiti, Simone Marins
Neosporosis
Neospora caninum
Reproductive Problems
Dairy cattle
Seropositive
Abortion.
Neosporose
Problemas reprodutivos
Soropositivo
Aborto.
Spiti, Simone Marins
Neosporosis
Neospora caninum
Reproductive Problems
Dairy cattle
Seropositive
Abortion.
Neosporose
Problemas reprodutivos
Soropositivo
Aborto.
title_short Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
title_full Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
title_fullStr Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
title_full_unstemmed Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
title_sort Occurrence of antibodies against Neospora caninum in cattle and dogs from dairy farms from the central northern region of Paraná State
author Spiti, Simone Marins
author_facet Spiti, Simone Marins
Spiti, Simone Marins
Barros, Luiz Daniel de
Seixas, Mércia de
Minutti, Ana Flavia
Martins, Thais Agostinho
Cunha Filho, Luiz Fernando Coelho da
Cardim, Sergio Tosi
Kono, Isabelli Sayuri
Nino, Beatriz de Souza Lima
Bogado, Alexey Leon Gomel
Garcia, João Luis
Barros, Luiz Daniel de
Seixas, Mércia de
Minutti, Ana Flavia
Martins, Thais Agostinho
Cunha Filho, Luiz Fernando Coelho da
Cardim, Sergio Tosi
Kono, Isabelli Sayuri
Nino, Beatriz de Souza Lima
Bogado, Alexey Leon Gomel
Garcia, João Luis
author_role author
author2 Barros, Luiz Daniel de
Seixas, Mércia de
Minutti, Ana Flavia
Martins, Thais Agostinho
Cunha Filho, Luiz Fernando Coelho da
Cardim, Sergio Tosi
Kono, Isabelli Sayuri
Nino, Beatriz de Souza Lima
Bogado, Alexey Leon Gomel
Garcia, João Luis
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Spiti, Simone Marins
Barros, Luiz Daniel de
Seixas, Mércia de
Minutti, Ana Flavia
Martins, Thais Agostinho
Cunha Filho, Luiz Fernando Coelho da
Cardim, Sergio Tosi
Kono, Isabelli Sayuri
Nino, Beatriz de Souza Lima
Bogado, Alexey Leon Gomel
Garcia, João Luis
dc.subject.por.fl_str_mv Neosporosis
Neospora caninum
Reproductive Problems
Dairy cattle
Seropositive
Abortion.
Neosporose
Problemas reprodutivos
Soropositivo
Aborto.
topic Neosporosis
Neospora caninum
Reproductive Problems
Dairy cattle
Seropositive
Abortion.
Neosporose
Problemas reprodutivos
Soropositivo
Aborto.
description Neosporosis is one of the principal causes of reproductive problems in cattle worldwide. The aim of this study was to evaluate the occurrence of anti-Neospora caninum antibodies in cattle and dogs from dairy farms from the central northern region of Paraná state. Blood samples with and without EDTA were collected from 400 cattle and 46 dogs from 20 properties. Nested polymerase chain reaction (n-PCR) was performed on whole blood samples and indirect immunofluorescence reaction (IFR) on the serum samples (after clot retraction). Cattle and dogs with titers ? 100 and ? 50, respectively, were considered positive. Anti-N. caninum was detected in 20,1% (80/400) of cattle, with titers ranging from 100 to 1600. The n-PCR presented only two cattle positives (0.5%). Anti-N. caninum was detected in 19,6% (9/46) of the dogs, with titers ranging from 50 to 6400. The occurrence of antibodies against N. caninum obtained in the present study was similar to those in studies performed in other regions of Paraná and Brazil. The probability of detecting parasitemia in epidemiological studies is a rare event.
publishDate 2018
dc.date.none.fl_str_mv 2018-11-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa científica
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31812
10.5433/1679-0359.2018v39n6p2449
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31812
identifier_str_mv 10.5433/1679-0359.2018v39n6p2449
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/31812/24636
dc.rights.driver.fl_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 39 No. 6 (2018); 2449-2456
Semina: Ciências Agrárias; v. 39 n. 6 (2018); 2449-2456
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1822182759161921536
dc.identifier.doi.none.fl_str_mv 10.5433/1679-0359.2018v39n6p2449

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