Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation

Detalhes bibliográficos
Autor(a) principal: Sousa, Francisco Carlos de
Data de Publicação: 2016
Outros Autores: Melo, Carlos Henrique Sousa de, Batista, Ribrio Ivan Tavares Pereira, Sanchez, Deisy Johana Diaz, Souza-Fabjan, Joanna Maria Gonçalves, Pereira, Alexsandra Fernandes, Melo, Luciana Magalhães, Freitas, Vicente José de Figueirêdo, Teixeira, Dárcio Ítalo Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23744
Resumo: In transgenic murine models, the study of certain organs or tissues can be performed after euthanasia of some specimens. However, this practice may not be economically feasible when applied to livestock such as transgenic goats. It is necessary to use minimally invasive methods to perform in vivo studies of organs that may be affected by disorders related to the activity of the transgene, particularly during milk production, when the recombinant protein is secreted. The aim of this study was to describe ultrasonographic findings of the liver, gallbladder, spleen, kidneys, and mammary glands in transgenic goats for evaluating the effect of human granulocyte-colony stimulating factor (hG-CSF) expression in milk during induced lactation. Six female Canindé goats-three transgenic (T) and three non-transgenic (NT)-were subjected to hormone therapy to induce lactation; ultrasonographic examinations of the liver, gallbladder, spleen, kidneys, and mammary gland were performed during both the hormonal therapy and the lactation period at different intervals depending on the organ being examined. On Day 16 (Day 1 = hormonal therapy initiation), all goats were lactating and presented healthy mammary glands, characterized by echogenic parenchyma showing a granular echotexture. Transgenic and non-transgenic goats were compared on the basis of measurements and ultrasound images obtained from each organ. No differences between T and NT animals were observed in the examined area for the liver, gallbladder, spleen, and kidneys. Liver and renal echogenicity and appearance of gallbladder and portal and hepatic veins were similar in all females. Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats did not show any difference from those in non-transgenic goats.Thus, these results suggest that the presence and expression of the transgene had no effect on the ultrasonographic findings of mammary gland and abdominal organs in the transgenic goats. Moreover, the findings shows that ultrasonography is a useful screening tool for clinical examination of transgenic goats; this allows the investigation of possible disorders and avoids the unnecessary use of invasive techniques.
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spelling Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactationAchados ultrassonográficos de glândula mamária, fígado, vesícula biliar, baço e rins de caprinos transgênicos para o hG-CSF durante lactação induzidaCaprineTransgenesisUltrasonographyAbdominal organs.CaprinoTransgêneseUltrassonografiaÓrgãos abdominais.In transgenic murine models, the study of certain organs or tissues can be performed after euthanasia of some specimens. However, this practice may not be economically feasible when applied to livestock such as transgenic goats. It is necessary to use minimally invasive methods to perform in vivo studies of organs that may be affected by disorders related to the activity of the transgene, particularly during milk production, when the recombinant protein is secreted. The aim of this study was to describe ultrasonographic findings of the liver, gallbladder, spleen, kidneys, and mammary glands in transgenic goats for evaluating the effect of human granulocyte-colony stimulating factor (hG-CSF) expression in milk during induced lactation. Six female Canindé goats-three transgenic (T) and three non-transgenic (NT)-were subjected to hormone therapy to induce lactation; ultrasonographic examinations of the liver, gallbladder, spleen, kidneys, and mammary gland were performed during both the hormonal therapy and the lactation period at different intervals depending on the organ being examined. On Day 16 (Day 1 = hormonal therapy initiation), all goats were lactating and presented healthy mammary glands, characterized by echogenic parenchyma showing a granular echotexture. Transgenic and non-transgenic goats were compared on the basis of measurements and ultrasound images obtained from each organ. No differences between T and NT animals were observed in the examined area for the liver, gallbladder, spleen, and kidneys. Liver and renal echogenicity and appearance of gallbladder and portal and hepatic veins were similar in all females. Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats did not show any difference from those in non-transgenic goats.Thus, these results suggest that the presence and expression of the transgene had no effect on the ultrasonographic findings of mammary gland and abdominal organs in the transgenic goats. Moreover, the findings shows that ultrasonography is a useful screening tool for clinical examination of transgenic goats; this allows the investigation of possible disorders and avoids the unnecessary use of invasive techniques.Em camundongos transgênicos, o estudo de certos órgãos ou tecidos pode ser viabilizado por meio da eutanásia de alguns exemplares. Entretanto, esta prática pode não ser economicamente viável quando aplicada a animais de produção, como caprinos transgênicos. É necessário utilizar métodos minimamente invasivos para estudar in vivo os órgãos que podem estar envolvidos em desordens relacionadas à atividade do transgene, principalmente durante a produção de leite, quando a proteína recombinante é secretada. O objetivo deste estudo foi descrever as características ultrassonográficas de fígado, vesícula biliar, baço, rins e glândula mamária de caprinos transgênicos expressando o fator estimulante de colônias de granulócitos humano (hG-CSF) no leite, durante lactação induzida. Seis fêmeas caprinas da raça Canindé: três não transgênicas (NT) e três transgênicas (T) foram submetidas a tratamento hormonal para indução da lactação; os exames ultrassonográficos de fígado, vesícula biliar, baço, rins e glândula mamária foram realizados durante tratamento hormonal e lactação, em diferentes intervalos de acordo com o órgão. No dia 16 (Dia 1 = início do tratamento hormonal) todas as cabras estavam em lactação e apresentando uma glândula mamária saudável, caracterizada por parênquima ecogênico, com uma ecotextura granular. As cabras T e NT foram comparadas em relação às mensurações e aparência ultrassonográfica obtida de cada órgão. Não foram observadas diferenças com relação à área para exame de fígado, vesícula biliar, baço e rins entre T e NT. A ecogenicidade de fígado e rins, a aparência da vesícula biliar e das veias porta e hepática foram similares em todas as fêmeas. As mensurações ultrassonográficas dos órgãos abdominais examinados não revelaram diferenças entre animais T e NT. Assim, esses resultados sugerem que a presença e expressão do transgene não tiveram efeito sobre as características ultrassonográficas de glândula mamária e órgãos abdominais nos animais transgênicos. Além disso, os achados mostram que a ultrassonografia é uma importante ferramenta para o exame clínico em caprinos transgênicos e que permite a investigação de possíveis desordens, evitando o uso desnecessário de técnicas invasivas.UEL2016-12-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2374410.5433/1679-0359.2016v37n6p4109Semina: Ciências Agrárias; Vol. 37 No. 6 (2016); 4109-4118Semina: Ciências Agrárias; v. 37 n. 6 (2016); 4109-41181679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23744/20063Copyright (c) 2016 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSousa, Francisco Carlos deMelo, Carlos Henrique Sousa deBatista, Ribrio Ivan Tavares PereiraSanchez, Deisy Johana DiazSouza-Fabjan, Joanna Maria GonçalvesPereira, Alexsandra FernandesMelo, Luciana MagalhãesFreitas, Vicente José de FigueirêdoTeixeira, Dárcio Ítalo Alves2022-11-29T15:56:37Zoai:ojs.pkp.sfu.ca:article/23744Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-29T15:56:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
Achados ultrassonográficos de glândula mamária, fígado, vesícula biliar, baço e rins de caprinos transgênicos para o hG-CSF durante lactação induzida
title Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
spellingShingle Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
Sousa, Francisco Carlos de
Caprine
Transgenesis
Ultrasonography
Abdominal organs.
Caprino
Transgênese
Ultrassonografia
Órgãos abdominais.
title_short Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
title_full Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
title_fullStr Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
title_full_unstemmed Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
title_sort Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats for hG-CSF during induced lactation
author Sousa, Francisco Carlos de
author_facet Sousa, Francisco Carlos de
Melo, Carlos Henrique Sousa de
Batista, Ribrio Ivan Tavares Pereira
Sanchez, Deisy Johana Diaz
Souza-Fabjan, Joanna Maria Gonçalves
Pereira, Alexsandra Fernandes
Melo, Luciana Magalhães
Freitas, Vicente José de Figueirêdo
Teixeira, Dárcio Ítalo Alves
author_role author
author2 Melo, Carlos Henrique Sousa de
Batista, Ribrio Ivan Tavares Pereira
Sanchez, Deisy Johana Diaz
Souza-Fabjan, Joanna Maria Gonçalves
Pereira, Alexsandra Fernandes
Melo, Luciana Magalhães
Freitas, Vicente José de Figueirêdo
Teixeira, Dárcio Ítalo Alves
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Francisco Carlos de
Melo, Carlos Henrique Sousa de
Batista, Ribrio Ivan Tavares Pereira
Sanchez, Deisy Johana Diaz
Souza-Fabjan, Joanna Maria Gonçalves
Pereira, Alexsandra Fernandes
Melo, Luciana Magalhães
Freitas, Vicente José de Figueirêdo
Teixeira, Dárcio Ítalo Alves
dc.subject.por.fl_str_mv Caprine
Transgenesis
Ultrasonography
Abdominal organs.
Caprino
Transgênese
Ultrassonografia
Órgãos abdominais.
topic Caprine
Transgenesis
Ultrasonography
Abdominal organs.
Caprino
Transgênese
Ultrassonografia
Órgãos abdominais.
description In transgenic murine models, the study of certain organs or tissues can be performed after euthanasia of some specimens. However, this practice may not be economically feasible when applied to livestock such as transgenic goats. It is necessary to use minimally invasive methods to perform in vivo studies of organs that may be affected by disorders related to the activity of the transgene, particularly during milk production, when the recombinant protein is secreted. The aim of this study was to describe ultrasonographic findings of the liver, gallbladder, spleen, kidneys, and mammary glands in transgenic goats for evaluating the effect of human granulocyte-colony stimulating factor (hG-CSF) expression in milk during induced lactation. Six female Canindé goats-three transgenic (T) and three non-transgenic (NT)-were subjected to hormone therapy to induce lactation; ultrasonographic examinations of the liver, gallbladder, spleen, kidneys, and mammary gland were performed during both the hormonal therapy and the lactation period at different intervals depending on the organ being examined. On Day 16 (Day 1 = hormonal therapy initiation), all goats were lactating and presented healthy mammary glands, characterized by echogenic parenchyma showing a granular echotexture. Transgenic and non-transgenic goats were compared on the basis of measurements and ultrasound images obtained from each organ. No differences between T and NT animals were observed in the examined area for the liver, gallbladder, spleen, and kidneys. Liver and renal echogenicity and appearance of gallbladder and portal and hepatic veins were similar in all females. Ultrasonographic findings of the mammary gland, liver, gallbladder, spleen, and kidneys in transgenic goats did not show any difference from those in non-transgenic goats.Thus, these results suggest that the presence and expression of the transgene had no effect on the ultrasonographic findings of mammary gland and abdominal organs in the transgenic goats. Moreover, the findings shows that ultrasonography is a useful screening tool for clinical examination of transgenic goats; this allows the investigation of possible disorders and avoids the unnecessary use of invasive techniques.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23744
10.5433/1679-0359.2016v37n6p4109
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23744
identifier_str_mv 10.5433/1679-0359.2016v37n6p4109
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/23744/20063
dc.rights.driver.fl_str_mv Copyright (c) 2016 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2016 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 37 No. 6 (2016); 4109-4118
Semina: Ciências Agrárias; v. 37 n. 6 (2016); 4109-4118
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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