Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico

Detalhes bibliográficos
Autor(a) principal: Nascimento, Renata Vieira do
Data de Publicação: 2021
Outros Autores: Pereira, Vanessa Alves, Almeida-Monteiro, Priscila Silva, Sales, Yara Silvino, Araújo, Ianna Wivianne Fernandes de, Rodrigues, José Ariévilo Gurgel, Costa, Thaís de Oliveira, Oliveira, Andressa Gomes de, Montenegro, Assis Rubens, Salmito-Vanderley, Carminda Sandra Brito
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
DOI: 10.5433/1679-0359.2021v42n5p2959
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826
Resumo: The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium.
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spelling Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachicoUso de glicosaminoglicanos da pele Oreochromis niloticus como suplemento antioxidante na criopreservação de sêmen de curimatã comum (Prochilodus brevis)CryopreservationOxidative stressRheophilic fishSpermatozoa kineticsSulfated glycans.Cinética espermáticaCriopreservaçãoEstresse oxidativoGlicanos sulfatadosPeixe reofílico.The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium.O estudo teve como objetivo avaliar in vitro a ação antioxidante de glicosaminoglicanos (GAGs) da pele de Oreochromis niloticus e determinar sua concentração ideal para suplementar o meio de congelação espermático de Prochilodus brevis. No experimento 1, foram verificadas as propriedades antioxidantes in vitro dos GAGs por meio das análises de DPPH, capacidade quelante do ferro e capacidade antioxidante total. No experimento 2, foram formados pool de sêmen, que foram congelados em solução suplementada, ou não, com diferentes concentrações de GAGs: 0 (controle); 0,5; 1,0; 1,5; 2,0; 2,5; 3,0; 3,5; 4,0; 4,5 ou 5,0 mg mL-1 (total de 10 tratamentos). As amostras foram avaliadas quanto à integridade da membrana, integridade do DNA, morfologia e cinética espermática. Os resultados do experimento 1, mostraram que os GAGs exibiram, com o aumento da concentração, ação antioxidante significativa, para todos os testes avaliados, principalmente na capacidade quelante do ferro. No experimento 2, observou-se que o aumento da concentração de GAGs diminuiu os parâmetros cinéticos (P < 0,05), porém o controle e a concentração de 0,5 mg mL-1 de GAGs apresentaram resultados semelhantes. Para os demais parâmetros (morfologia, integridade de membrana e de DNA), não houve diminuição dos resultados com o aumento da concentração de GAGs. Em conclusão, os GAGs, extraídos da pele de O. niloticus, possuem ação antioxidante, sendo a concentração de 0,5 mg mL-1 a mais adequada para suplementar o meio de congelação espermático de P. brevis.UEL2021-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4182610.5433/1679-0359.2021v42n5p2959Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 2959-2978Semina: Ciências Agrárias; v. 42 n. 5 (2021); 2959-29781679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826/29962Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessNascimento, Renata Vieira doPereira, Vanessa AlvesAlmeida-Monteiro, Priscila SilvaSales, Yara SilvinoAraújo, Ianna Wivianne Fernandes deRodrigues, José Ariévilo GurgelCosta, Thaís de OliveiraOliveira, Andressa Gomes deMontenegro, Assis RubensSalmito-Vanderley, Carminda Sandra Brito2022-09-30T13:31:54Zoai:ojs.pkp.sfu.ca:article/41826Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T13:31:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
Uso de glicosaminoglicanos da pele Oreochromis niloticus como suplemento antioxidante na criopreservação de sêmen de curimatã comum (Prochilodus brevis)
title Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
spellingShingle Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
Nascimento, Renata Vieira do
Cryopreservation
Oxidative stress
Rheophilic fish
Spermatozoa kinetics
Sulfated glycans.
Cinética espermática
Criopreservação
Estresse oxidativo
Glicanos sulfatados
Peixe reofílico.
Nascimento, Renata Vieira do
Cryopreservation
Oxidative stress
Rheophilic fish
Spermatozoa kinetics
Sulfated glycans.
Cinética espermática
Criopreservação
Estresse oxidativo
Glicanos sulfatados
Peixe reofílico.
title_short Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
title_full Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
title_fullStr Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
title_full_unstemmed Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
title_sort Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
author Nascimento, Renata Vieira do
author_facet Nascimento, Renata Vieira do
Nascimento, Renata Vieira do
Pereira, Vanessa Alves
Almeida-Monteiro, Priscila Silva
Sales, Yara Silvino
Araújo, Ianna Wivianne Fernandes de
Rodrigues, José Ariévilo Gurgel
Costa, Thaís de Oliveira
Oliveira, Andressa Gomes de
Montenegro, Assis Rubens
Salmito-Vanderley, Carminda Sandra Brito
Pereira, Vanessa Alves
Almeida-Monteiro, Priscila Silva
Sales, Yara Silvino
Araújo, Ianna Wivianne Fernandes de
Rodrigues, José Ariévilo Gurgel
Costa, Thaís de Oliveira
Oliveira, Andressa Gomes de
Montenegro, Assis Rubens
Salmito-Vanderley, Carminda Sandra Brito
author_role author
author2 Pereira, Vanessa Alves
Almeida-Monteiro, Priscila Silva
Sales, Yara Silvino
Araújo, Ianna Wivianne Fernandes de
Rodrigues, José Ariévilo Gurgel
Costa, Thaís de Oliveira
Oliveira, Andressa Gomes de
Montenegro, Assis Rubens
Salmito-Vanderley, Carminda Sandra Brito
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nascimento, Renata Vieira do
Pereira, Vanessa Alves
Almeida-Monteiro, Priscila Silva
Sales, Yara Silvino
Araújo, Ianna Wivianne Fernandes de
Rodrigues, José Ariévilo Gurgel
Costa, Thaís de Oliveira
Oliveira, Andressa Gomes de
Montenegro, Assis Rubens
Salmito-Vanderley, Carminda Sandra Brito
dc.subject.por.fl_str_mv Cryopreservation
Oxidative stress
Rheophilic fish
Spermatozoa kinetics
Sulfated glycans.
Cinética espermática
Criopreservação
Estresse oxidativo
Glicanos sulfatados
Peixe reofílico.
topic Cryopreservation
Oxidative stress
Rheophilic fish
Spermatozoa kinetics
Sulfated glycans.
Cinética espermática
Criopreservação
Estresse oxidativo
Glicanos sulfatados
Peixe reofílico.
description The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826
10.5433/1679-0359.2021v42n5p2959
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826
identifier_str_mv 10.5433/1679-0359.2021v42n5p2959
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826/29962
dc.rights.driver.fl_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 2959-2978
Semina: Ciências Agrárias; v. 42 n. 5 (2021); 2959-2978
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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dc.identifier.doi.none.fl_str_mv 10.5433/1679-0359.2021v42n5p2959