Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
DOI: | 10.5433/1679-0359.2021v42n5p2959 |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826 |
Resumo: | The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium. |
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Semina. Ciências Agrárias (Online) |
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Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachicoUso de glicosaminoglicanos da pele Oreochromis niloticus como suplemento antioxidante na criopreservação de sêmen de curimatã comum (Prochilodus brevis)CryopreservationOxidative stressRheophilic fishSpermatozoa kineticsSulfated glycans.Cinética espermáticaCriopreservaçãoEstresse oxidativoGlicanos sulfatadosPeixe reofílico.The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium.O estudo teve como objetivo avaliar in vitro a ação antioxidante de glicosaminoglicanos (GAGs) da pele de Oreochromis niloticus e determinar sua concentração ideal para suplementar o meio de congelação espermático de Prochilodus brevis. No experimento 1, foram verificadas as propriedades antioxidantes in vitro dos GAGs por meio das análises de DPPH, capacidade quelante do ferro e capacidade antioxidante total. No experimento 2, foram formados pool de sêmen, que foram congelados em solução suplementada, ou não, com diferentes concentrações de GAGs: 0 (controle); 0,5; 1,0; 1,5; 2,0; 2,5; 3,0; 3,5; 4,0; 4,5 ou 5,0 mg mL-1 (total de 10 tratamentos). As amostras foram avaliadas quanto à integridade da membrana, integridade do DNA, morfologia e cinética espermática. Os resultados do experimento 1, mostraram que os GAGs exibiram, com o aumento da concentração, ação antioxidante significativa, para todos os testes avaliados, principalmente na capacidade quelante do ferro. No experimento 2, observou-se que o aumento da concentração de GAGs diminuiu os parâmetros cinéticos (P < 0,05), porém o controle e a concentração de 0,5 mg mL-1 de GAGs apresentaram resultados semelhantes. Para os demais parâmetros (morfologia, integridade de membrana e de DNA), não houve diminuição dos resultados com o aumento da concentração de GAGs. Em conclusão, os GAGs, extraídos da pele de O. niloticus, possuem ação antioxidante, sendo a concentração de 0,5 mg mL-1 a mais adequada para suplementar o meio de congelação espermático de P. brevis.UEL2021-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4182610.5433/1679-0359.2021v42n5p2959Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 2959-2978Semina: Ciências Agrárias; v. 42 n. 5 (2021); 2959-29781679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826/29962Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessNascimento, Renata Vieira doPereira, Vanessa AlvesAlmeida-Monteiro, Priscila SilvaSales, Yara SilvinoAraújo, Ianna Wivianne Fernandes deRodrigues, José Ariévilo GurgelCosta, Thaís de OliveiraOliveira, Andressa Gomes deMontenegro, Assis RubensSalmito-Vanderley, Carminda Sandra Brito2022-09-30T13:31:54Zoai:ojs.pkp.sfu.ca:article/41826Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T13:31:54Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico Uso de glicosaminoglicanos da pele Oreochromis niloticus como suplemento antioxidante na criopreservação de sêmen de curimatã comum (Prochilodus brevis) |
title |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico |
spellingShingle |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico Nascimento, Renata Vieira do Cryopreservation Oxidative stress Rheophilic fish Spermatozoa kinetics Sulfated glycans. Cinética espermática Criopreservação Estresse oxidativo Glicanos sulfatados Peixe reofílico. Nascimento, Renata Vieira do Cryopreservation Oxidative stress Rheophilic fish Spermatozoa kinetics Sulfated glycans. Cinética espermática Criopreservação Estresse oxidativo Glicanos sulfatados Peixe reofílico. |
title_short |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico |
title_full |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico |
title_fullStr |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico |
title_full_unstemmed |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico |
title_sort |
Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico |
author |
Nascimento, Renata Vieira do |
author_facet |
Nascimento, Renata Vieira do Nascimento, Renata Vieira do Pereira, Vanessa Alves Almeida-Monteiro, Priscila Silva Sales, Yara Silvino Araújo, Ianna Wivianne Fernandes de Rodrigues, José Ariévilo Gurgel Costa, Thaís de Oliveira Oliveira, Andressa Gomes de Montenegro, Assis Rubens Salmito-Vanderley, Carminda Sandra Brito Pereira, Vanessa Alves Almeida-Monteiro, Priscila Silva Sales, Yara Silvino Araújo, Ianna Wivianne Fernandes de Rodrigues, José Ariévilo Gurgel Costa, Thaís de Oliveira Oliveira, Andressa Gomes de Montenegro, Assis Rubens Salmito-Vanderley, Carminda Sandra Brito |
author_role |
author |
author2 |
Pereira, Vanessa Alves Almeida-Monteiro, Priscila Silva Sales, Yara Silvino Araújo, Ianna Wivianne Fernandes de Rodrigues, José Ariévilo Gurgel Costa, Thaís de Oliveira Oliveira, Andressa Gomes de Montenegro, Assis Rubens Salmito-Vanderley, Carminda Sandra Brito |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento, Renata Vieira do Pereira, Vanessa Alves Almeida-Monteiro, Priscila Silva Sales, Yara Silvino Araújo, Ianna Wivianne Fernandes de Rodrigues, José Ariévilo Gurgel Costa, Thaís de Oliveira Oliveira, Andressa Gomes de Montenegro, Assis Rubens Salmito-Vanderley, Carminda Sandra Brito |
dc.subject.por.fl_str_mv |
Cryopreservation Oxidative stress Rheophilic fish Spermatozoa kinetics Sulfated glycans. Cinética espermática Criopreservação Estresse oxidativo Glicanos sulfatados Peixe reofílico. |
topic |
Cryopreservation Oxidative stress Rheophilic fish Spermatozoa kinetics Sulfated glycans. Cinética espermática Criopreservação Estresse oxidativo Glicanos sulfatados Peixe reofílico. |
description |
The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826 10.5433/1679-0359.2021v42n5p2959 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826 |
identifier_str_mv |
10.5433/1679-0359.2021v42n5p2959 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/41826/29962 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 5 (2021); 2959-2978 Semina: Ciências Agrárias; v. 42 n. 5 (2021); 2959-2978 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1822182691699687424 |
dc.identifier.doi.none.fl_str_mv |
10.5433/1679-0359.2021v42n5p2959 |