Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract

Detalhes bibliográficos
Autor(a) principal: Yoshihara, Eidi
Data de Publicação: 2015
Outros Autores: Minho, Alessandro Pelegrine, Tabacow, Victor Bittencourt Dutra, Cardim, Sérgio Tosi, Yamamura, Milton Hissashi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20391
Resumo: The parasite Haemonchus contortus is one of the most pathogenic for small ruminants in tropical and subtropical regions worldwide, including Brazil. The objective of this study was to evaluate the structural changes induced in adult H. contortus after in vitro contact with Acacia mearnsii extract (AE), using scanning electron microscopy. Adult nematodes were collected from a naturally infected lamb. In the in vitro assay the parasites were placed in contact with AE (100 mg ml-1), for two hours at 37oC. The nematodes used in the assays (exposed to AE and the negative controls) were analyzed using an electron scanning microscope (quadruplicate per treatment). In all replicates, similar morphological alterations were observed on the entire extension of the cuticle of the specimens that remained in contact with the EA in vitro assays, none significant lesion was observed in the negative control (not exposed to AE). These results indicate the direct action of EA on the cuticle of H. contortus in in vitro trials
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spelling Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extractAlterações ultraestruturais da cutícula de Haemonchus contortus expostos ao extrato de Acacia mearnsiiGastrointestinal nematodesScanning electron microscopyStructural changesTannins.Alterações ultraestruturaisMicroscopia eletrônicaNematódeos gastrintestinaisTaninos.The parasite Haemonchus contortus is one of the most pathogenic for small ruminants in tropical and subtropical regions worldwide, including Brazil. The objective of this study was to evaluate the structural changes induced in adult H. contortus after in vitro contact with Acacia mearnsii extract (AE), using scanning electron microscopy. Adult nematodes were collected from a naturally infected lamb. In the in vitro assay the parasites were placed in contact with AE (100 mg ml-1), for two hours at 37oC. The nematodes used in the assays (exposed to AE and the negative controls) were analyzed using an electron scanning microscope (quadruplicate per treatment). In all replicates, similar morphological alterations were observed on the entire extension of the cuticle of the specimens that remained in contact with the EA in vitro assays, none significant lesion was observed in the negative control (not exposed to AE). These results indicate the direct action of EA on the cuticle of H. contortus in in vitro trialsHaemonchus contortus é um dos parasitos mais patogênicos para pequenos ruminantes em regiões tropicais e subtropicais em todo o mundo, inclusive no Brasil. O objetivo do trabalho foi analisar alterações ultraestruturais na cutícula de H. contortus após contato com extrato de Acacia mearnsii. Os parasitos expostos ao extrato de Acacia mearnsii (EA) e os controles negativos foram analisados ao microscópio eletrônico de varredura. No teste in vitro, adultos de H. contortus foram coletados de um ovino naturalmente infectado, e os parasitos foram colocados em contato com o EA (100 mg mL-1), durante duas horas à 37oC. Os nematódeos expostos ao EA apresentaram alterações morfológicas em toda a extensão da cutícula. As alterações morfológicas não foram observadas no controle negativo. Estes resultados indicam a ação direta do EA na cutícula de H. contortus. UEL2015-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa de campoPesquisa de campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2039110.5433/1679-0359.2015v36n6p3763Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3763-3768Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3763-37681679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20391/17463http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessYoshihara, EidiMinho, Alessandro PelegrineTabacow, Victor Bittencourt DutraCardim, Sérgio TosiYamamura, Milton Hissashi2022-12-05T11:46:23Zoai:ojs.pkp.sfu.ca:article/20391Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-05T11:46:23Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
Alterações ultraestruturais da cutícula de Haemonchus contortus expostos ao extrato de Acacia mearnsii
title Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
spellingShingle Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
Yoshihara, Eidi
Gastrointestinal nematodes
Scanning electron microscopy
Structural changes
Tannins.
Alterações ultraestruturais
Microscopia eletrônica
Nematódeos gastrintestinais
Taninos.
title_short Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
title_full Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
title_fullStr Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
title_full_unstemmed Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
title_sort Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract
author Yoshihara, Eidi
author_facet Yoshihara, Eidi
Minho, Alessandro Pelegrine
Tabacow, Victor Bittencourt Dutra
Cardim, Sérgio Tosi
Yamamura, Milton Hissashi
author_role author
author2 Minho, Alessandro Pelegrine
Tabacow, Victor Bittencourt Dutra
Cardim, Sérgio Tosi
Yamamura, Milton Hissashi
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Yoshihara, Eidi
Minho, Alessandro Pelegrine
Tabacow, Victor Bittencourt Dutra
Cardim, Sérgio Tosi
Yamamura, Milton Hissashi
dc.subject.por.fl_str_mv Gastrointestinal nematodes
Scanning electron microscopy
Structural changes
Tannins.
Alterações ultraestruturais
Microscopia eletrônica
Nematódeos gastrintestinais
Taninos.
topic Gastrointestinal nematodes
Scanning electron microscopy
Structural changes
Tannins.
Alterações ultraestruturais
Microscopia eletrônica
Nematódeos gastrintestinais
Taninos.
description The parasite Haemonchus contortus is one of the most pathogenic for small ruminants in tropical and subtropical regions worldwide, including Brazil. The objective of this study was to evaluate the structural changes induced in adult H. contortus after in vitro contact with Acacia mearnsii extract (AE), using scanning electron microscopy. Adult nematodes were collected from a naturally infected lamb. In the in vitro assay the parasites were placed in contact with AE (100 mg ml-1), for two hours at 37oC. The nematodes used in the assays (exposed to AE and the negative controls) were analyzed using an electron scanning microscope (quadruplicate per treatment). In all replicates, similar morphological alterations were observed on the entire extension of the cuticle of the specimens that remained in contact with the EA in vitro assays, none significant lesion was observed in the negative control (not exposed to AE). These results indicate the direct action of EA on the cuticle of H. contortus in in vitro trials
publishDate 2015
dc.date.none.fl_str_mv 2015-12-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa de campo
Pesquisa de campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20391
10.5433/1679-0359.2015v36n6p3763
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20391
identifier_str_mv 10.5433/1679-0359.2015v36n6p3763
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20391/17463
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 6 (2015); 3763-3768
Semina: Ciências Agrárias; v. 36 n. 6 (2015); 3763-3768
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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