Influencing factors for preparation of platelet-rich plasma in horses

Detalhes bibliográficos
Autor(a) principal: Pedroso, Ana Carolina Barros da Rosa
Data de Publicação: 2021
Outros Autores: Peixoto, Andréia da Costa, Oliveira, Evelyn de, Dutra, Helena Tavares, Basile, Roberta Carvalho, Brandstetter, Luciana Ramos Gaston, Moura, Veridiana Maria Brianezi Dignani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39503
Resumo: There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition method or the ideal platelet concentration to stimulate tissue repair. Besides that, most studies do not provide a clear and replicable description of the methods used, which makes standardization and result comparison difficult. Thus, this study aimed to accurately describe the method and evaluate factors influencing PRP preparation in equines. In this regard, two protocols were used, P1 and P2, based on two centrifugation methods, which differed in blood volume, speed and time, and platelet pipetting and resuspension. In conclusion, factors such as centrifugation strength and time are essential to obtain PRP with adequate platelet count. However, other factors such as tube type and plasma pipetting and resuspension methods directly interfere with the replicability of the technique, and hence influencing PRP applicability.
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spelling Influencing factors for preparation of platelet-rich plasma in horsesFatores que influenciam o preparo do plasma rico em plaquetas na espécie equinaPlatelet concentrationDouble centrifugation.Standardization.Concentração plaquetáriaDupla centrifugaçãoPadronização.There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition method or the ideal platelet concentration to stimulate tissue repair. Besides that, most studies do not provide a clear and replicable description of the methods used, which makes standardization and result comparison difficult. Thus, this study aimed to accurately describe the method and evaluate factors influencing PRP preparation in equines. In this regard, two protocols were used, P1 and P2, based on two centrifugation methods, which differed in blood volume, speed and time, and platelet pipetting and resuspension. In conclusion, factors such as centrifugation strength and time are essential to obtain PRP with adequate platelet count. However, other factors such as tube type and plasma pipetting and resuspension methods directly interfere with the replicability of the technique, and hence influencing PRP applicability.Não há consenso na literatura quanto ao melhor método de obtenção do plasma rico em plaquetas (PRP) ou à concentração plaquetária ideal com o objetivo de estimular o reparo tecidual. Além disso, grande parte dos trabalhos não faz descrição clara e reprodutível dos métodos utilizados, o que dificulta a padronização e a comparação dos resultados. Dessa forma, este trabalho teve por objetivo descrever detalhadamente a metodologia e avaliar fatores que influenciam o preparo do PRP em equinos. Para isso, foram utilizados dois protocolos, P1 e P2, baseados em duas centrifugações, que diferiram quanto ao volume de sangue colhido, velocidade e tempo das centrifugações e método de pipetagem e ressuspensão das plaquetas. Conclui-se que fatores como força e tempo de centrifugação são essenciais à obtenção de um PRP com concentração plaquetária adequada. Contudo, fatores adicionais como tipo de tubo e métodos de pipetagem e ressuspensão do plasma interferem diretamente na repetibilidade da técnica, influenciando a aplicabilidade prática do PRP.UEL2021-05-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3950310.5433/1679-0359.2021v42n4p2327Semina: Ciências Agrárias; Vol. 42 No. 4 (2021); 2327-2338Semina: Ciências Agrárias; v. 42 n. 4 (2021); 2327-23381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39503/29661Copyright (c) 2021 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessPedroso, Ana Carolina Barros da RosaPeixoto, Andréia da CostaOliveira, Evelyn deDutra, Helena TavaresBasile, Roberta CarvalhoBrandstetter, Luciana Ramos GastonMoura, Veridiana Maria Brianezi Dignani de2022-09-30T14:13:37Zoai:ojs.pkp.sfu.ca:article/39503Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-09-30T14:13:37Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Influencing factors for preparation of platelet-rich plasma in horses
Fatores que influenciam o preparo do plasma rico em plaquetas na espécie equina
title Influencing factors for preparation of platelet-rich plasma in horses
spellingShingle Influencing factors for preparation of platelet-rich plasma in horses
Pedroso, Ana Carolina Barros da Rosa
Platelet concentration
Double centrifugation.
Standardization.
Concentração plaquetária
Dupla centrifugação
Padronização.
title_short Influencing factors for preparation of platelet-rich plasma in horses
title_full Influencing factors for preparation of platelet-rich plasma in horses
title_fullStr Influencing factors for preparation of platelet-rich plasma in horses
title_full_unstemmed Influencing factors for preparation of platelet-rich plasma in horses
title_sort Influencing factors for preparation of platelet-rich plasma in horses
author Pedroso, Ana Carolina Barros da Rosa
author_facet Pedroso, Ana Carolina Barros da Rosa
Peixoto, Andréia da Costa
Oliveira, Evelyn de
Dutra, Helena Tavares
Basile, Roberta Carvalho
Brandstetter, Luciana Ramos Gaston
Moura, Veridiana Maria Brianezi Dignani de
author_role author
author2 Peixoto, Andréia da Costa
Oliveira, Evelyn de
Dutra, Helena Tavares
Basile, Roberta Carvalho
Brandstetter, Luciana Ramos Gaston
Moura, Veridiana Maria Brianezi Dignani de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pedroso, Ana Carolina Barros da Rosa
Peixoto, Andréia da Costa
Oliveira, Evelyn de
Dutra, Helena Tavares
Basile, Roberta Carvalho
Brandstetter, Luciana Ramos Gaston
Moura, Veridiana Maria Brianezi Dignani de
dc.subject.por.fl_str_mv Platelet concentration
Double centrifugation.
Standardization.
Concentração plaquetária
Dupla centrifugação
Padronização.
topic Platelet concentration
Double centrifugation.
Standardization.
Concentração plaquetária
Dupla centrifugação
Padronização.
description There is no consensus in the literature as to the best platelet-rich plasma (PRP) acquisition method or the ideal platelet concentration to stimulate tissue repair. Besides that, most studies do not provide a clear and replicable description of the methods used, which makes standardization and result comparison difficult. Thus, this study aimed to accurately describe the method and evaluate factors influencing PRP preparation in equines. In this regard, two protocols were used, P1 and P2, based on two centrifugation methods, which differed in blood volume, speed and time, and platelet pipetting and resuspension. In conclusion, factors such as centrifugation strength and time are essential to obtain PRP with adequate platelet count. However, other factors such as tube type and plasma pipetting and resuspension methods directly interfere with the replicability of the technique, and hence influencing PRP applicability.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39503
10.5433/1679-0359.2021v42n4p2327
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39503
identifier_str_mv 10.5433/1679-0359.2021v42n4p2327
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/39503/29661
dc.rights.driver.fl_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 42 No. 4 (2021); 2327-2338
Semina: Ciências Agrárias; v. 42 n. 4 (2021); 2327-2338
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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