Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38048 |
Resumo: | Salinity and water deficiency are factors that limit the initial development of crops, directly interfering with the efficiency of food production. Studies on the behavior of cultivable species under stress are important to determine management actions; therefore, the mechanisms involved in post-stress recovery should be investigated. The objective of this study was to evaluate the effect of water and salt stress on enzymatic activity in azuki bean seedlings. The experimental design was a completely randomized, 4 × 6 factorial arrangement (four reagents: CaCl2, KCl, NaCl, and polyethylene glycol 6000 (PEG 6000) × six osmotic potentials: 0.0, -0.2, -0.4, -0.8, -1.2, and -1.6 MPa). The quantification of protein content and analysis of enzyme (catalase, peroxidase, and phenylalanine ammonia-lyase) activity in seedlings were performed on the 10th day after sowing. NaCl is toxic and recovery of azuki bean seedlings was less likely under salt stress owing to reduced enzymatic activity. In contrast, seedlings subjected to KCl treatment showed increased production of antioxidant enzymes. |
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Semina. Ciências Agrárias (Online) |
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Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiencyAtividade enzimática em plântulas de feijão-azuki submetidas a salinidade e a restrição hídricaAntioxidant enzymesVigna angularis L.Water stressSalinity stress.Enzimas antioxidantesVigna angularis L. Estresse hídricoEstresse salino.Salinity and water deficiency are factors that limit the initial development of crops, directly interfering with the efficiency of food production. Studies on the behavior of cultivable species under stress are important to determine management actions; therefore, the mechanisms involved in post-stress recovery should be investigated. The objective of this study was to evaluate the effect of water and salt stress on enzymatic activity in azuki bean seedlings. The experimental design was a completely randomized, 4 × 6 factorial arrangement (four reagents: CaCl2, KCl, NaCl, and polyethylene glycol 6000 (PEG 6000) × six osmotic potentials: 0.0, -0.2, -0.4, -0.8, -1.2, and -1.6 MPa). The quantification of protein content and analysis of enzyme (catalase, peroxidase, and phenylalanine ammonia-lyase) activity in seedlings were performed on the 10th day after sowing. NaCl is toxic and recovery of azuki bean seedlings was less likely under salt stress owing to reduced enzymatic activity. In contrast, seedlings subjected to KCl treatment showed increased production of antioxidant enzymes.A salinidade e a restrição hídrica são fatores limitantes ao desenvolvimento inicial das culturas, interferindo diretamente na eficiência da produção de alimentos. Estudos sobre o comportamento das espécies cultiváveis sob estresse são importantes para determinar ações de manejo, por essa razão deve haver a investigação e compreensão dos mecanismos envolvidos na recuperação pós estresse. O objetivo do trabalho foi avaliar o efeito do estresse hídrico e salino sobre a atividade enzimática de plântulas feijão-azuki. O delineamento experimental utilizado foi o inteiramente casualizado, em arranjo fatorial 4x6 (4 reagentes: CaCl2, KCl, NaCl e polietilenoglicol 6000 (PEG 6000) x 6 potenciais osmóticos 0,0; -0,2; -0,4; -0,8; -1,2; -1,6 MPa). A quantificação do teor de proteínas e atividade das enzimas catalase, peroxidase e fenilalanina amônia-liase de plântulas foram analisadas no 10º dia após semeadura. O NaCl é tóxico e possibilita menores chances de recuperação em plântulas de feijão-azuki sob estresse salino devido a menor atividade enzimática. Por outro lado, as plântulas submetidas à solução de KCl apresentam aumento da produção de enzimas antioxidantes.UEL2021-01-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3804810.5433/1679-0359.2021v42n1p419Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 419-428Semina: Ciências Agrárias; v. 42 n. 1 (2021); 419-4281679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38048/28520Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessOmura, Mônica SatieFreiria, Gustavo HenriquePellizzaro, VerônicaBertoncelli, Douglas JuniorFurlan, Felipe FavorettoOliveira, Eli Carlos deTakahashi, Lúcia Sadayo Assari2022-10-04T15:55:41Zoai:ojs.pkp.sfu.ca:article/38048Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-04T15:55:41Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency Atividade enzimática em plântulas de feijão-azuki submetidas a salinidade e a restrição hídrica |
title |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency |
spellingShingle |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency Omura, Mônica Satie Antioxidant enzymes Vigna angularis L. Water stress Salinity stress. Enzimas antioxidantes Vigna angularis L. Estresse hídrico Estresse salino. |
title_short |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency |
title_full |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency |
title_fullStr |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency |
title_full_unstemmed |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency |
title_sort |
Enzymatic activity in azuki bean seedlings subjected to salinity and water deficiency |
author |
Omura, Mônica Satie |
author_facet |
Omura, Mônica Satie Freiria, Gustavo Henrique Pellizzaro, Verônica Bertoncelli, Douglas Junior Furlan, Felipe Favoretto Oliveira, Eli Carlos de Takahashi, Lúcia Sadayo Assari |
author_role |
author |
author2 |
Freiria, Gustavo Henrique Pellizzaro, Verônica Bertoncelli, Douglas Junior Furlan, Felipe Favoretto Oliveira, Eli Carlos de Takahashi, Lúcia Sadayo Assari |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Omura, Mônica Satie Freiria, Gustavo Henrique Pellizzaro, Verônica Bertoncelli, Douglas Junior Furlan, Felipe Favoretto Oliveira, Eli Carlos de Takahashi, Lúcia Sadayo Assari |
dc.subject.por.fl_str_mv |
Antioxidant enzymes Vigna angularis L. Water stress Salinity stress. Enzimas antioxidantes Vigna angularis L. Estresse hídrico Estresse salino. |
topic |
Antioxidant enzymes Vigna angularis L. Water stress Salinity stress. Enzimas antioxidantes Vigna angularis L. Estresse hídrico Estresse salino. |
description |
Salinity and water deficiency are factors that limit the initial development of crops, directly interfering with the efficiency of food production. Studies on the behavior of cultivable species under stress are important to determine management actions; therefore, the mechanisms involved in post-stress recovery should be investigated. The objective of this study was to evaluate the effect of water and salt stress on enzymatic activity in azuki bean seedlings. The experimental design was a completely randomized, 4 × 6 factorial arrangement (four reagents: CaCl2, KCl, NaCl, and polyethylene glycol 6000 (PEG 6000) × six osmotic potentials: 0.0, -0.2, -0.4, -0.8, -1.2, and -1.6 MPa). The quantification of protein content and analysis of enzyme (catalase, peroxidase, and phenylalanine ammonia-lyase) activity in seedlings were performed on the 10th day after sowing. NaCl is toxic and recovery of azuki bean seedlings was less likely under salt stress owing to reduced enzymatic activity. In contrast, seedlings subjected to KCl treatment showed increased production of antioxidant enzymes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-19 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38048 10.5433/1679-0359.2021v42n1p419 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38048 |
identifier_str_mv |
10.5433/1679-0359.2021v42n1p419 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38048/28520 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 42 No. 1 (2021); 419-428 Semina: Ciências Agrárias; v. 42 n. 1 (2021); 419-428 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1750315430731317248 |