Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru

Detalhes bibliográficos
Autor(a) principal: Temoche, Luis Fernando Cerro
Data de Publicação: 2018
Outros Autores: Seabra Junior, Eloy da Silva, Cordeiro, Matheus Dias, Fonseca, Adivaldo Henrique da, Cunha, Nathalie Costa da, Almosny, Nadia Regina Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29052
Resumo: The aim of this study was to determine the frequency of ticks positive for genus Rickettsia bacteria among ticks collected from domestic dogs in the Department of Piura, Peru, using polymerase chain reaction (PCR) analysis. Ticks were collected from dogs in urban areas of the metropolitan region of Piura, Peru. Only three species of ticks were identified; 977 Rhipicephalus sanguineus (180 nymphs, 417 females, and 380 males), Six Amblyomma triste females, and one Amblyomma tigrinum male. After classifying the specimens morphologically by stage, species, and sex, their total DNA was tested by PCR using primers that amplify fragments of the gltA, ompA, ompB, and htrA genes. The resulting positive sample was sequenced, compared to the GenBank database, and analyzed phylogenetically. The Rickettsia spp. infection rate in the tick pools was 0.2% (1/484); the positive specimen was an R. sanguineus tick. GenBank analysis of the positive sequence revealed 100% identify with Rickettsia felis; however, no products of the htrA, ompA and ompB genes were amplified from this sample. To the best of our knowledge, this is the first report of R. felis in R. sanguineus in Peru.
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spelling Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, PeruDetecção molecular de Rickettsia spp. em carrapatos coletados de cães provenientes do Departamento de Piura-PeruAcariIxodidaeMolecular analysisRickettsiosisSouth America.AcariIxodidaeAnálise molecularRickettsiosisAmérica do Sul.The aim of this study was to determine the frequency of ticks positive for genus Rickettsia bacteria among ticks collected from domestic dogs in the Department of Piura, Peru, using polymerase chain reaction (PCR) analysis. Ticks were collected from dogs in urban areas of the metropolitan region of Piura, Peru. Only three species of ticks were identified; 977 Rhipicephalus sanguineus (180 nymphs, 417 females, and 380 males), Six Amblyomma triste females, and one Amblyomma tigrinum male. After classifying the specimens morphologically by stage, species, and sex, their total DNA was tested by PCR using primers that amplify fragments of the gltA, ompA, ompB, and htrA genes. The resulting positive sample was sequenced, compared to the GenBank database, and analyzed phylogenetically. The Rickettsia spp. infection rate in the tick pools was 0.2% (1/484); the positive specimen was an R. sanguineus tick. GenBank analysis of the positive sequence revealed 100% identify with Rickettsia felis; however, no products of the htrA, ompA and ompB genes were amplified from this sample. To the best of our knowledge, this is the first report of R. felis in R. sanguineus in Peru.O objetivo do presente estudo foi determinar a frequência de carrapatos coletados de caninos domésticos positivos para bactérias do gênero Rickettsia por meio da Reação em cadeia da polimerase (PCR) no Departamento de Piura, Peru. Desta forma, carrapatos foram coletados de caninos de zonas urbanas da região Metropolitana de Piura, Peru. Apenas três espécies de carrapatos foram identificadas, sendo 977 R. sanguineus (180 ninfas, 417 fêmeas e 380 machos), 6 fêmeas da espécie Amblyomma triste e um macho Amblyomma tigrinum. Após classificados morfologicamente por estádio, espécie e sexo, o DNA total dos espécimes foi testado pela PCR utilizando iniciadores que amplificam fragmentos dos genes gltA, ompA, ompB e htrA. A amostra positiva foi sequenciada, comparada com o banco de dados depositados no GenBank e analisada filogeneticamente. Verificou-se, mediante PCR, uma taxa de infecção de 0,2% (1/484) “pools” de carrapatos positivos para Rickettsia spp. O espécime positivo foi um carrapato Rhipicephalus sanguineus. A análise no GenBank da sequência positiva apresentou uma similaridade de 100% com a espécie R. felis. Essa amostra não amplificou produtos para os genes htrA, ompA e ompB. Este é o primeiro relato de R. felis em R. sanguineus no Peru.UEL2018-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2905210.5433/1679-0359.2018v39n2p605Semina: Ciências Agrárias; Vol. 39 No. 2 (2018); 605-612Semina: Ciências Agrárias; v. 39 n. 2 (2018); 605-6121679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29052/23193Copyright (c) 2018 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessTemoche, Luis Fernando CerroSeabra Junior, Eloy da SilvaCordeiro, Matheus DiasFonseca, Adivaldo Henrique daCunha, Nathalie Costa daAlmosny, Nadia Regina Pereira2022-10-20T21:29:11Zoai:ojs.pkp.sfu.ca:article/29052Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-20T21:29:11Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
Detecção molecular de Rickettsia spp. em carrapatos coletados de cães provenientes do Departamento de Piura-Peru
title Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
spellingShingle Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
Temoche, Luis Fernando Cerro
Acari
Ixodidae
Molecular analysis
Rickettsiosis
South America.
Acari
Ixodidae
Análise molecular
Rickettsiosis
América do Sul.
title_short Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
title_full Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
title_fullStr Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
title_full_unstemmed Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
title_sort Molecular detection of Rickettsia spp. in ticks collected from dogs from the Department of Piura, Peru
author Temoche, Luis Fernando Cerro
author_facet Temoche, Luis Fernando Cerro
Seabra Junior, Eloy da Silva
Cordeiro, Matheus Dias
Fonseca, Adivaldo Henrique da
Cunha, Nathalie Costa da
Almosny, Nadia Regina Pereira
author_role author
author2 Seabra Junior, Eloy da Silva
Cordeiro, Matheus Dias
Fonseca, Adivaldo Henrique da
Cunha, Nathalie Costa da
Almosny, Nadia Regina Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Temoche, Luis Fernando Cerro
Seabra Junior, Eloy da Silva
Cordeiro, Matheus Dias
Fonseca, Adivaldo Henrique da
Cunha, Nathalie Costa da
Almosny, Nadia Regina Pereira
dc.subject.por.fl_str_mv Acari
Ixodidae
Molecular analysis
Rickettsiosis
South America.
Acari
Ixodidae
Análise molecular
Rickettsiosis
América do Sul.
topic Acari
Ixodidae
Molecular analysis
Rickettsiosis
South America.
Acari
Ixodidae
Análise molecular
Rickettsiosis
América do Sul.
description The aim of this study was to determine the frequency of ticks positive for genus Rickettsia bacteria among ticks collected from domestic dogs in the Department of Piura, Peru, using polymerase chain reaction (PCR) analysis. Ticks were collected from dogs in urban areas of the metropolitan region of Piura, Peru. Only three species of ticks were identified; 977 Rhipicephalus sanguineus (180 nymphs, 417 females, and 380 males), Six Amblyomma triste females, and one Amblyomma tigrinum male. After classifying the specimens morphologically by stage, species, and sex, their total DNA was tested by PCR using primers that amplify fragments of the gltA, ompA, ompB, and htrA genes. The resulting positive sample was sequenced, compared to the GenBank database, and analyzed phylogenetically. The Rickettsia spp. infection rate in the tick pools was 0.2% (1/484); the positive specimen was an R. sanguineus tick. GenBank analysis of the positive sequence revealed 100% identify with Rickettsia felis; however, no products of the htrA, ompA and ompB genes were amplified from this sample. To the best of our knowledge, this is the first report of R. felis in R. sanguineus in Peru.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-15
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29052
10.5433/1679-0359.2018v39n2p605
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29052
identifier_str_mv 10.5433/1679-0359.2018v39n2p605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/29052/23193
dc.rights.driver.fl_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 39 No. 2 (2018); 605-612
Semina: Ciências Agrárias; v. 39 n. 2 (2018); 605-612
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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