Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures

Detalhes bibliográficos
Autor(a) principal: Zorzetti, Janaina
Data de Publicação: 2014
Outros Autores: Santoto, Patricia Helena, Constanski, Kelly Christiane, Neves, Pedro Manuel Oliveira Janeiro
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14641
Resumo: The aim of this study was to evaluate the effect of in vitro successive subcultures of Beauveria bassiana isolates in different nutritional conditions on conidia heat and UV radiation tolerance and on sensitivity to azoxystrobin fungicide. The fungus was initially inoculated in Alphitobius diaperinus (Coleoptera: Tenebrionidae) adults and after its development, the conidia were cultivated in PDA (potato dextrose agar) and MPE (medium to produce Beauveria spp. conidia) originating the first subculture 1st (A) conidia, which were successively multiplied in their media until the 20th cultivation. Conidia taken from the 20th subculture were again inoculated in the insect and after sporulation they were once more multiplied in the media, producing the 1st (B) conidia. For the tests, 1st (A), 10th, 20th and 1st (B) conidia from the CG 152 and Unioeste 40 isolates were used. Successive subcultures and the medium nutritional conditions interfered on the fungus physiology, and the isolates showed different behaviors. The subcultures on PDA showed higher thermotolerance to Unioeste 40 in all subcultures and to CG 152 just after inoculation in the host. B. bassiana was susceptible to UV radiation, and this characteristic was influenced by the subcultures in different media. Both isolates were more tolerant to UV radiation when subcultivated on MPE, while just for the 40 Unioeste this tolerance increased after host inoculation. The B. bassiana sensitivity to azoxystrobin fungicide was affected after successive subcultures in both medium. Conidia originated in MPE were more resistant to fungicide and the host inoculation increased this tolerance. These results show that it is possible to enhance the fungi efficiency to control pests, through the selection of a virulent isolate, and handling their nutritional conditions during cultivation and host inoculation.
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spelling Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subculturesSensibilidade de conídios de Beauveria bassiana a fatores abióticos após sucessivos cultivos in vitroBiological controlEntomopathogenic fungiUV radiation toleranceHeat tolerance.Controle biológicoFungos entomopatogênicosTolerância à radiação UVTolerância à temperatura.Controle biológicoThe aim of this study was to evaluate the effect of in vitro successive subcultures of Beauveria bassiana isolates in different nutritional conditions on conidia heat and UV radiation tolerance and on sensitivity to azoxystrobin fungicide. The fungus was initially inoculated in Alphitobius diaperinus (Coleoptera: Tenebrionidae) adults and after its development, the conidia were cultivated in PDA (potato dextrose agar) and MPE (medium to produce Beauveria spp. conidia) originating the first subculture 1st (A) conidia, which were successively multiplied in their media until the 20th cultivation. Conidia taken from the 20th subculture were again inoculated in the insect and after sporulation they were once more multiplied in the media, producing the 1st (B) conidia. For the tests, 1st (A), 10th, 20th and 1st (B) conidia from the CG 152 and Unioeste 40 isolates were used. Successive subcultures and the medium nutritional conditions interfered on the fungus physiology, and the isolates showed different behaviors. The subcultures on PDA showed higher thermotolerance to Unioeste 40 in all subcultures and to CG 152 just after inoculation in the host. B. bassiana was susceptible to UV radiation, and this characteristic was influenced by the subcultures in different media. Both isolates were more tolerant to UV radiation when subcultivated on MPE, while just for the 40 Unioeste this tolerance increased after host inoculation. The B. bassiana sensitivity to azoxystrobin fungicide was affected after successive subcultures in both medium. Conidia originated in MPE were more resistant to fungicide and the host inoculation increased this tolerance. These results show that it is possible to enhance the fungi efficiency to control pests, through the selection of a virulent isolate, and handling their nutritional conditions during cultivation and host inoculation.O objetivo deste trabalho foi avaliar o efeito de sucessivos cultivos in vitro de isolados de Beauveria bassiana, em diferentes condições nutricionais, sobre a tolerância ao calor e à radiação UV e a sensibilidade ao fungicida azoxistrobina. O fungo foi inicialmente inoculado em adultos de Alphitobius diaperinus (Coleoptera: Tenebrionidae), e após o seu desenvolvimento, os conídios foram multiplicados nos meios BDA (batata, dextrose, ágar) e MPE (meio para a produção de esporos de Beauveria spp.), originando os conídios de primeiro cultivo 1°(A), os quais foram sucessivamente multiplicados nos respectivos meios até o 20° cultivo. Os conídios retirados do 20º cultivo foram reinoculados no inseto, e após a esporulação, foram novamente multiplicados nos meios de origem, produzindo os conídios de 1°(B). Foram selecionados para os ensaios, os conídios de 1º(A), 10º, 20º e 1º(B) cultivos dos isolados CG 152 e Unioeste 40. Os cultivos sucessivos e as condições nutricionais do meio interferiram na fisiologia do fungo, e os isolados mostraram comportamentos diferentes entre si. Os cultivos em meio BDA proporcionaram maior termotolerância ao Unioeste 40 em todos os cultivos, e ao CG 152 apenas após as inoculações no hospedeiro. B. bassiana foi suscetível à radiação UV, e essa característica foi influenciada pelos cultivos nos diferentes meios. Ambos isolados foram mais tolerantes a radiação UV quando cultivados em MPE, enquanto apenas para o Unioeste 40 essa tolerância aumentou após a inoculação no hospedeiro. A sensibilidade de B. bassiana ao fungicida azoxistrobina foi afetada após os sucessivos cultivos em ambos os meios. Os conídios originados em MPE foram mais resistentes ao fungicida, e a inoculação no hospedeiro também aumentou essa tolerância. Esses resultados mostram que é possível aprimorar a eficiência de fungos no controle de pragas, através da seleção de um isolado virulento, manipulação de suas condições nutricionais durante o cultivo e da inoculação no hospedeiro.UEL2014-08-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo científicoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1464110.5433/1679-0359.2014v35n4p1773Semina: Ciências Agrárias; Vol. 35 No. 4 (2014); 1773-1784Semina: Ciências Agrárias; v. 35 n. 4 (2014); 1773-17841679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14641/pdf_410Zorzetti, JanainaSantoto, Patricia HelenaConstanski, Kelly ChristianeNeves, Pedro Manuel Oliveira Janeiroinfo:eu-repo/semantics/openAccess2015-11-19T18:35:32Zoai:ojs.pkp.sfu.ca:article/14641Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:35:32Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
Sensibilidade de conídios de Beauveria bassiana a fatores abióticos após sucessivos cultivos in vitro
title Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
spellingShingle Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
Zorzetti, Janaina
Biological control
Entomopathogenic fungi
UV radiation tolerance
Heat tolerance.
Controle biológico
Fungos entomopatogênicos
Tolerância à radiação UV
Tolerância à temperatura.
Controle biológico
title_short Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
title_full Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
title_fullStr Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
title_full_unstemmed Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
title_sort Sensitivity of Beauveria bassiana conidia to abiotic factors after successive in vitro subcultures
author Zorzetti, Janaina
author_facet Zorzetti, Janaina
Santoto, Patricia Helena
Constanski, Kelly Christiane
Neves, Pedro Manuel Oliveira Janeiro
author_role author
author2 Santoto, Patricia Helena
Constanski, Kelly Christiane
Neves, Pedro Manuel Oliveira Janeiro
author2_role author
author
author
dc.contributor.author.fl_str_mv Zorzetti, Janaina
Santoto, Patricia Helena
Constanski, Kelly Christiane
Neves, Pedro Manuel Oliveira Janeiro
dc.subject.por.fl_str_mv Biological control
Entomopathogenic fungi
UV radiation tolerance
Heat tolerance.
Controle biológico
Fungos entomopatogênicos
Tolerância à radiação UV
Tolerância à temperatura.
Controle biológico
topic Biological control
Entomopathogenic fungi
UV radiation tolerance
Heat tolerance.
Controle biológico
Fungos entomopatogênicos
Tolerância à radiação UV
Tolerância à temperatura.
Controle biológico
description The aim of this study was to evaluate the effect of in vitro successive subcultures of Beauveria bassiana isolates in different nutritional conditions on conidia heat and UV radiation tolerance and on sensitivity to azoxystrobin fungicide. The fungus was initially inoculated in Alphitobius diaperinus (Coleoptera: Tenebrionidae) adults and after its development, the conidia were cultivated in PDA (potato dextrose agar) and MPE (medium to produce Beauveria spp. conidia) originating the first subculture 1st (A) conidia, which were successively multiplied in their media until the 20th cultivation. Conidia taken from the 20th subculture were again inoculated in the insect and after sporulation they were once more multiplied in the media, producing the 1st (B) conidia. For the tests, 1st (A), 10th, 20th and 1st (B) conidia from the CG 152 and Unioeste 40 isolates were used. Successive subcultures and the medium nutritional conditions interfered on the fungus physiology, and the isolates showed different behaviors. The subcultures on PDA showed higher thermotolerance to Unioeste 40 in all subcultures and to CG 152 just after inoculation in the host. B. bassiana was susceptible to UV radiation, and this characteristic was influenced by the subcultures in different media. Both isolates were more tolerant to UV radiation when subcultivated on MPE, while just for the 40 Unioeste this tolerance increased after host inoculation. The B. bassiana sensitivity to azoxystrobin fungicide was affected after successive subcultures in both medium. Conidia originated in MPE were more resistant to fungicide and the host inoculation increased this tolerance. These results show that it is possible to enhance the fungi efficiency to control pests, through the selection of a virulent isolate, and handling their nutritional conditions during cultivation and host inoculation.
publishDate 2014
dc.date.none.fl_str_mv 2014-08-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo científico
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14641
10.5433/1679-0359.2014v35n4p1773
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14641
identifier_str_mv 10.5433/1679-0359.2014v35n4p1773
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14641/pdf_410
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 35 No. 4 (2014); 1773-1784
Semina: Ciências Agrárias; v. 35 n. 4 (2014); 1773-1784
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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