Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508 |
Resumo: | Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP. |
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Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gelQuantificação de colágenoem feridas dérmicas de coelhos tratados com plasma rico em plaquetas heterólogo gelWound healingRabbitsCollagenPlatelet-rich plasma.Cicatrização de feridasCoelhoColágenoPlasma rico em plaquetas.Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP.O plasma rico em plaquetas (PRP) tem sido amplamente estudado como um biomaterial para o tratamento de feridas, sendo o produto de fonte heteróloga indicado na impossibilidade de obtenção do sangue do próprio paciente. O presente estudo teve como objetivo, avaliar e comparar a cicatrização em coelhos, e quantificar o colágeno de feridas experimentalmente induzidas, denominadas controle e tratadas, com PRP heterólogo gel. A hipótese é que este produto seja capaz de promover cicatrização adequada sem reações adversas, e promova aumento do colágeno. Os aspectos clínicos cor, edema, hiperemia, exsudato, crosta, granulação, sensibilidade a dor e índice de retração das feridas foram avaliados nos dias 7, 14 e 17 após a lesão. A quantificação do colágeno, através da coloração com Picrosirius e avaliação sob luz polarizada, foi realizada no 17º dia. Houve presença de crosta nos dois grupos em todos os momentos avaliados, com ausência dos demais sinais clínicos. O percentual de contração da lesão e quantidade de colágeno não diferiu entre os grupos. Concluiu-se que a hipótese sugerida foi comprovada em partes, ou seja, o PRP heterólogo gel não foi capaz de aumentar a quantidade de colágeno e acelerar o processo de cicatrização tecidual, no entanto, a cicatrização foi eficiente e semelhante entre os dois grupos e não houve nenhum tipo de reação adversa local. Desta forma, apesar da escassez de estudos encontrados na literatura, o PRP heterólogo gel é uma alternativa de tratamento de feridas, na impossibilidade de uso de outras fontes de PRP.UEL2017-03-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo científicoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2450810.5433/1679-0359.2017v38n1p249Semina: Ciências Agrárias; Vol. 38 No. 1 (2017); 249-258Semina: Ciências Agrárias; v. 38 n. 1 (2017); 249-2581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508/22162Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMarques, Maria Elisa MarinLaposy, Cecília BragaSilva, Mariana Lucas dos SantosBreda, Maria Rosa SantosMatsumoto, Mayara MascaroJustulin Junior, Luis AntonioNai, Gisele AlborghettiOzaki, Guilherme Akio TamuraCamargo Filho, José CarlosNogueira, Rosa Maria Barilli2022-11-29T14:33:24Zoai:ojs.pkp.sfu.ca:article/24508Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-29T14:33:24Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel Quantificação de colágenoem feridas dérmicas de coelhos tratados com plasma rico em plaquetas heterólogo gel |
title |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel |
spellingShingle |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel Marques, Maria Elisa Marin Wound healing Rabbits Collagen Platelet-rich plasma. Cicatrização de feridas Coelho Colágeno Plasma rico em plaquetas. |
title_short |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel |
title_full |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel |
title_fullStr |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel |
title_full_unstemmed |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel |
title_sort |
Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel |
author |
Marques, Maria Elisa Marin |
author_facet |
Marques, Maria Elisa Marin Laposy, Cecília Braga Silva, Mariana Lucas dos Santos Breda, Maria Rosa Santos Matsumoto, Mayara Mascaro Justulin Junior, Luis Antonio Nai, Gisele Alborghetti Ozaki, Guilherme Akio Tamura Camargo Filho, José Carlos Nogueira, Rosa Maria Barilli |
author_role |
author |
author2 |
Laposy, Cecília Braga Silva, Mariana Lucas dos Santos Breda, Maria Rosa Santos Matsumoto, Mayara Mascaro Justulin Junior, Luis Antonio Nai, Gisele Alborghetti Ozaki, Guilherme Akio Tamura Camargo Filho, José Carlos Nogueira, Rosa Maria Barilli |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Marques, Maria Elisa Marin Laposy, Cecília Braga Silva, Mariana Lucas dos Santos Breda, Maria Rosa Santos Matsumoto, Mayara Mascaro Justulin Junior, Luis Antonio Nai, Gisele Alborghetti Ozaki, Guilherme Akio Tamura Camargo Filho, José Carlos Nogueira, Rosa Maria Barilli |
dc.subject.por.fl_str_mv |
Wound healing Rabbits Collagen Platelet-rich plasma. Cicatrização de feridas Coelho Colágeno Plasma rico em plaquetas. |
topic |
Wound healing Rabbits Collagen Platelet-rich plasma. Cicatrização de feridas Coelho Colágeno Plasma rico em plaquetas. |
description |
Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-02 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artigo científico |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508 10.5433/1679-0359.2017v38n1p249 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508 |
identifier_str_mv |
10.5433/1679-0359.2017v38n1p249 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508/22162 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 1 (2017); 249-258 Semina: Ciências Agrárias; v. 38 n. 1 (2017); 249-258 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306075306983424 |