Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel

Detalhes bibliográficos
Autor(a) principal: Marques, Maria Elisa Marin
Data de Publicação: 2017
Outros Autores: Laposy, Cecília Braga, Silva, Mariana Lucas dos Santos, Breda, Maria Rosa Santos, Matsumoto, Mayara Mascaro, Justulin Junior, Luis Antonio, Nai, Gisele Alborghetti, Ozaki, Guilherme Akio Tamura, Camargo Filho, José Carlos, Nogueira, Rosa Maria Barilli
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508
Resumo: Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP.
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spelling Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gelQuantificação de colágenoem feridas dérmicas de coelhos tratados com plasma rico em plaquetas heterólogo gelWound healingRabbitsCollagenPlatelet-rich plasma.Cicatrização de feridasCoelhoColágenoPlasma rico em plaquetas.Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP.O plasma rico em plaquetas (PRP) tem sido amplamente estudado como um biomaterial para o tratamento de feridas, sendo o produto de fonte heteróloga indicado na impossibilidade de obtenção do sangue do próprio paciente. O presente estudo teve como objetivo, avaliar e comparar a cicatrização em coelhos, e quantificar o colágeno de feridas experimentalmente induzidas, denominadas controle e tratadas, com PRP heterólogo gel. A hipótese é que este produto seja capaz de promover cicatrização adequada sem reações adversas, e promova aumento do colágeno. Os aspectos clínicos cor, edema, hiperemia, exsudato, crosta, granulação, sensibilidade a dor e índice de retração das feridas foram avaliados nos dias 7, 14 e 17 após a lesão. A quantificação do colágeno, através da coloração com Picrosirius e avaliação sob luz polarizada, foi realizada no 17º dia. Houve presença de crosta nos dois grupos em todos os momentos avaliados, com ausência dos demais sinais clínicos. O percentual de contração da lesão e quantidade de colágeno não diferiu entre os grupos. Concluiu-se que a hipótese sugerida foi comprovada em partes, ou seja, o PRP heterólogo gel não foi capaz de aumentar a quantidade de colágeno e acelerar o processo de cicatrização tecidual, no entanto, a cicatrização foi eficiente e semelhante entre os dois grupos e não houve nenhum tipo de reação adversa local. Desta forma, apesar da escassez de estudos encontrados na literatura, o PRP heterólogo gel é uma alternativa de tratamento de feridas, na impossibilidade de uso de outras fontes de PRP.UEL2017-03-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArtigo científicoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2450810.5433/1679-0359.2017v38n1p249Semina: Ciências Agrárias; Vol. 38 No. 1 (2017); 249-258Semina: Ciências Agrárias; v. 38 n. 1 (2017); 249-2581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508/22162Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMarques, Maria Elisa MarinLaposy, Cecília BragaSilva, Mariana Lucas dos SantosBreda, Maria Rosa SantosMatsumoto, Mayara MascaroJustulin Junior, Luis AntonioNai, Gisele AlborghettiOzaki, Guilherme Akio TamuraCamargo Filho, José CarlosNogueira, Rosa Maria Barilli2022-11-29T14:33:24Zoai:ojs.pkp.sfu.ca:article/24508Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-29T14:33:24Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
Quantificação de colágenoem feridas dérmicas de coelhos tratados com plasma rico em plaquetas heterólogo gel
title Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
spellingShingle Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
Marques, Maria Elisa Marin
Wound healing
Rabbits
Collagen
Platelet-rich plasma.
Cicatrização de feridas
Coelho
Colágeno
Plasma rico em plaquetas.
title_short Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
title_full Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
title_fullStr Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
title_full_unstemmed Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
title_sort Collagen quantification in rabbit dermal wounds treated with heterologous platelet-rich plasma gel
author Marques, Maria Elisa Marin
author_facet Marques, Maria Elisa Marin
Laposy, Cecília Braga
Silva, Mariana Lucas dos Santos
Breda, Maria Rosa Santos
Matsumoto, Mayara Mascaro
Justulin Junior, Luis Antonio
Nai, Gisele Alborghetti
Ozaki, Guilherme Akio Tamura
Camargo Filho, José Carlos
Nogueira, Rosa Maria Barilli
author_role author
author2 Laposy, Cecília Braga
Silva, Mariana Lucas dos Santos
Breda, Maria Rosa Santos
Matsumoto, Mayara Mascaro
Justulin Junior, Luis Antonio
Nai, Gisele Alborghetti
Ozaki, Guilherme Akio Tamura
Camargo Filho, José Carlos
Nogueira, Rosa Maria Barilli
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Maria Elisa Marin
Laposy, Cecília Braga
Silva, Mariana Lucas dos Santos
Breda, Maria Rosa Santos
Matsumoto, Mayara Mascaro
Justulin Junior, Luis Antonio
Nai, Gisele Alborghetti
Ozaki, Guilherme Akio Tamura
Camargo Filho, José Carlos
Nogueira, Rosa Maria Barilli
dc.subject.por.fl_str_mv Wound healing
Rabbits
Collagen
Platelet-rich plasma.
Cicatrização de feridas
Coelho
Colágeno
Plasma rico em plaquetas.
topic Wound healing
Rabbits
Collagen
Platelet-rich plasma.
Cicatrização de feridas
Coelho
Colágeno
Plasma rico em plaquetas.
description Platelet-rich plasma (PRP) has been extensively studied as a biomaterial for wound treatment, and the heterologous PRP is usefulin the event that obtaining the patient’s own blood is impossible. This study aimed to evaluate and compare wound healing in rabbits and quantify the collagen in experimentally induced wounds in a control group and in a group treated with heterologous PRP gel. We hypothesize that this gelis capable of promoting proper healing with no adverse reactions, increased collagen content. The clinical aspects of coloring, edema, hyperemia, exudation, crust, granulation, pain sensitivity, and retraction index of the wounds were measuredon days 7, 14, and 17 after the injury. Collagen quantification by Picrosirius staining and evaluation under polarized light was performed on the 17th day. Crust was present in both groups at all evaluated time points, with the absence of other clinical signs. The wound contraction rate and collagen quantity did not differ between groups. In conclusion, the suggested hypothesis was partially confirmed; the heterologous PRP gel was unable to increase the amount of collagen and accelerate the wound healing process, however, wound healing was efficient and similar in both groups and there was no local adverse reaction. Thus, despite the scarcity of studies in the literature, the heterologous PRP gel is an effective alternative treatment for wounds in the absence of other sources of PRP.
publishDate 2017
dc.date.none.fl_str_mv 2017-03-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Artigo científico
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508
10.5433/1679-0359.2017v38n1p249
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508
identifier_str_mv 10.5433/1679-0359.2017v38n1p249
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/24508/22162
dc.rights.driver.fl_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 38 No. 1 (2017); 249-258
Semina: Ciências Agrárias; v. 38 n. 1 (2017); 249-258
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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