Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication

Detalhes bibliográficos
Autor(a) principal: Fogaça, Luciana Alves
Data de Publicação: 2016
Outros Autores: Pedrotti, Enio Luiz, Alves, Antonio Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22608
Resumo: For conventional micropropagation methods, semisolidified medium (SM) is used; the use of this medium requires intense manipulation of the cultures and skilled labor. Systems that use liquid medium show equal or better efficiency of the multiplication process, besides reducing the cost for the elimination of agar. In this study, we evaluated the mass propagation of Agapanthus umbellatus var. minor two in vitro multiplication systems (SM system and temporary immersion system [SIT]). The plant material was grown in MS medium supplemented with 6-benzylaminopurine (6-BA; 0.0, 8.9, 17.8, and 35.6 ?M). The data obtained in this study demonstrate that the two systems used were efficient for the multiplication phase of this species. However, we recommend SIT in view of its reuse in the process of multiplication and rooting. Moreover, simple construction, low cost of the culture medium, and low cost of the bioreactors and the fact that agar is not required qualify this system as an efficient alternative for large-scale micropropagation of Agapanthus umbellatus var. minor. We recommend 17.8 ?M 6-BA for the SM system and 8.9 ?M 6-BA for SIT.
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spelling Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplicationMicropropagação de Agapanthus umbellatus var. minor com dois sistemas de multiplicaçãoIn vitro cultureTemporary immersionSemisolidified mediumCytokine.Cultura in vitroImersão temporáriaMeio geleificadoCitocinina.For conventional micropropagation methods, semisolidified medium (SM) is used; the use of this medium requires intense manipulation of the cultures and skilled labor. Systems that use liquid medium show equal or better efficiency of the multiplication process, besides reducing the cost for the elimination of agar. In this study, we evaluated the mass propagation of Agapanthus umbellatus var. minor two in vitro multiplication systems (SM system and temporary immersion system [SIT]). The plant material was grown in MS medium supplemented with 6-benzylaminopurine (6-BA; 0.0, 8.9, 17.8, and 35.6 ?M). The data obtained in this study demonstrate that the two systems used were efficient for the multiplication phase of this species. However, we recommend SIT in view of its reuse in the process of multiplication and rooting. Moreover, simple construction, low cost of the culture medium, and low cost of the bioreactors and the fact that agar is not required qualify this system as an efficient alternative for large-scale micropropagation of Agapanthus umbellatus var. minor. We recommend 17.8 ?M 6-BA for the SM system and 8.9 ?M 6-BA for SIT.Na metodologia convencional de micropropagação utiliza-se meio nutritivo geleificado, o que acarreta intensa manipulação das culturas e envolve mão-de-obra especializada. Porém, há uma tendência em se utilizar biorreatores em meio líquido com igual ou até melhor eficiência do processo de multiplicação, além de diminuir o custo pela eliminação do ágar. O presente trabalho teve como objetivo comparar a eficiência do uso do meio geleificado (MG) e de biorreator de imersão temporária (BIT), visando a propagação em massa de Agapanthus umbellatus var. minor. O material vegetal cresceu em meio MS suplementado com diferentes concentrações de 6-BAP (0,0; 8,9; 17,8 e 35,6 ?M). As avaliações de crescimento e desenvolvimento aos 60 dias mostraram que ambos os sistemas foram eficientes na fase de multiplicação. No entanto, a imersão temporária pode ser recomendada, com vista à sua reutilização no processo de multiplicação e enraizamento. Além disso, sugere-se este sistema como uma alternativa eficiente para a micropropagação em larga escala desta espécie por sua simplicidade na construção, custo mais baixo do meio de cultura, por não utilizar ágar e pelo baixo custo dos biorreatores. Dentre as concentrações utilizadas nos sistemas, recomenda-se a concentração de 17,8 ?M de 6-BAP para o MG e 8,9 ?M de 6-BAP para o BIT.UEL2016-10-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de CampoPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2260810.5433/1679-0359.2016v37n5p2923Semina: Ciências Agrárias; Vol. 37 No. 5 (2016); 2923-2932Semina: Ciências Agrárias; v. 37 n. 5 (2016); 2923-29321679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22608/19746http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessFogaça, Luciana AlvesPedrotti, Enio LuizAlves, Antonio Carlos2022-11-30T13:14:41Zoai:ojs.pkp.sfu.ca:article/22608Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-11-30T13:14:41Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
Micropropagação de Agapanthus umbellatus var. minor com dois sistemas de multiplicação
title Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
spellingShingle Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
Fogaça, Luciana Alves
In vitro culture
Temporary immersion
Semisolidified medium
Cytokine.
Cultura in vitro
Imersão temporária
Meio geleificado
Citocinina.
title_short Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
title_full Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
title_fullStr Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
title_full_unstemmed Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
title_sort Micropropagation of Agapanthus umbellatus var. minor by using two systems of multiplication
author Fogaça, Luciana Alves
author_facet Fogaça, Luciana Alves
Pedrotti, Enio Luiz
Alves, Antonio Carlos
author_role author
author2 Pedrotti, Enio Luiz
Alves, Antonio Carlos
author2_role author
author
dc.contributor.author.fl_str_mv Fogaça, Luciana Alves
Pedrotti, Enio Luiz
Alves, Antonio Carlos
dc.subject.por.fl_str_mv In vitro culture
Temporary immersion
Semisolidified medium
Cytokine.
Cultura in vitro
Imersão temporária
Meio geleificado
Citocinina.
topic In vitro culture
Temporary immersion
Semisolidified medium
Cytokine.
Cultura in vitro
Imersão temporária
Meio geleificado
Citocinina.
description For conventional micropropagation methods, semisolidified medium (SM) is used; the use of this medium requires intense manipulation of the cultures and skilled labor. Systems that use liquid medium show equal or better efficiency of the multiplication process, besides reducing the cost for the elimination of agar. In this study, we evaluated the mass propagation of Agapanthus umbellatus var. minor two in vitro multiplication systems (SM system and temporary immersion system [SIT]). The plant material was grown in MS medium supplemented with 6-benzylaminopurine (6-BA; 0.0, 8.9, 17.8, and 35.6 ?M). The data obtained in this study demonstrate that the two systems used were efficient for the multiplication phase of this species. However, we recommend SIT in view of its reuse in the process of multiplication and rooting. Moreover, simple construction, low cost of the culture medium, and low cost of the bioreactors and the fact that agar is not required qualify this system as an efficient alternative for large-scale micropropagation of Agapanthus umbellatus var. minor. We recommend 17.8 ?M 6-BA for the SM system and 8.9 ?M 6-BA for SIT.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Empírica de Campo
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22608
10.5433/1679-0359.2016v37n5p2923
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22608
identifier_str_mv 10.5433/1679-0359.2016v37n5p2923
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/22608/19746
dc.rights.driver.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 37 No. 5 (2016); 2923-2932
Semina: Ciências Agrárias; v. 37 n. 5 (2016); 2923-2932
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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