Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33461 |
Resumo: | In Brazil, the most important tickborne pathogens affecting dogs include Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis. Babesia spp. and Hepatozoon spp., transmitted by ixodid ticks, have been reported to naturally infect dogs and are widespread. The authors aimed to investigate the incidence of B. vogeli and Hepatozoon spp. infection using molecular methods to identify factors associated with the infection in dogs from urban areas of Cuiabá municipality, Midwestern Brazil. Polymerase chain reaction (PCR) assay revealed a prevalence of 9.36% (Confidence Interval-CI 95%; 2.72%; 6.79%) and 9.61% (CI 95%; 7.0%; 13.0%) among dogs for B. vogeli and Hepatozoon, respectively. DNA sequences obtained from 10 Hepatozoon PCR positive samples were sequenced and were identical to one another and, moreover, were 100% (541/541 base of pairs-bp) homologous to the corresponding 18S rDNA sequences of H. canis. Twenty-five dogs (6.02%) generated amplicons using PCR protocols for both organisms, indicating co-infection by these two protozoans. To the best of our knowledge, our study was the first molecular survey to consider the entire population of dogs from the study area. Moreover, young dogs (0-12 months of age), as well as animals living in walled houses?without access to the street?were more susceptible to infection with B. vogeli and H. canis, respectively. |
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Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern BrazilLevantamento molecular de Babesia vogeli e Hepatozoon spp. em cães da área urbana do Centro-Oeste do BrasilCanine babesiosisCanine hepatozoonosisPCRTick-borne diseases.Babesiose caninaHepatozoonose caninaPCRDoenças transmitidas por carrapatos.In Brazil, the most important tickborne pathogens affecting dogs include Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis. Babesia spp. and Hepatozoon spp., transmitted by ixodid ticks, have been reported to naturally infect dogs and are widespread. The authors aimed to investigate the incidence of B. vogeli and Hepatozoon spp. infection using molecular methods to identify factors associated with the infection in dogs from urban areas of Cuiabá municipality, Midwestern Brazil. Polymerase chain reaction (PCR) assay revealed a prevalence of 9.36% (Confidence Interval-CI 95%; 2.72%; 6.79%) and 9.61% (CI 95%; 7.0%; 13.0%) among dogs for B. vogeli and Hepatozoon, respectively. DNA sequences obtained from 10 Hepatozoon PCR positive samples were sequenced and were identical to one another and, moreover, were 100% (541/541 base of pairs-bp) homologous to the corresponding 18S rDNA sequences of H. canis. Twenty-five dogs (6.02%) generated amplicons using PCR protocols for both organisms, indicating co-infection by these two protozoans. To the best of our knowledge, our study was the first molecular survey to consider the entire population of dogs from the study area. Moreover, young dogs (0-12 months of age), as well as animals living in walled houses?without access to the street?were more susceptible to infection with B. vogeli and H. canis, respectively.No Brasil, os mais importantes patógenos transmitidos por carrapatos que acometem cães incluem Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis e Mycoplasma haemocanis. Babesia spp. e Hepatozoon spp., transmitidas por carrapatos ixodídeos, têm sido relatadas como capazes de infectar naturalmente cães e são amplamente distribuídos. Os autores objetivaram investigar a incidência de B. vogeli e Hepatozoon spp. por métodos moleculares para identificar fatores associados à infecção em cães da área urbana do município de Cuiabá, centro-oeste do Brasil. A reação em cadeia da polimerase (PCR) revelou uma prevalência de 9,36% (Intervalo de Confiança-IC 95%; 2,72%; 6,79%) e 9,61% (IC 95%; 7,0%; 13,0%) entre os cães para B. vogeli e Hepatozoon, respectivamente. Sequências de DNA obtidas de 10 amostras positivas para PCR de Hepatozoon foram sequenciados e eram idênticos entre si e, além disso, foram 100% (541/541 pares de base-pb) homólogos com a correspondente sequência de 18S rDNA de H. canis. Vinte e cinco (6,02%) cães geraram amplificados usando protocolos de PCR para ambos os organismos, indicando coinfecção por esses dois protozoários. Até onde sabemos, nosso estudo foi o primeiro levantamento molecular a considerar toda a população de cães da área de estudo na amostragem. Além disso, cães jovens (0-12 meses de idade), bem como animais que viviam em casas muradas - sem acesso à rua - foram mais suscetíveis à infecção por B. vogeli e H. canis, respectivamente.UEL2019-05-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3346110.5433/1679-0359.2019v40n3p1357Semina: Ciências Agrárias; Vol. 40 No. 3 (2019); 1357-1364Semina: Ciências Agrárias; v. 40 n. 3 (2019); 1357-13641679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33461/25432Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMaia, Maerle OliveiraFreitas, André Luís Santos deSantos, Jamila GuimarãesPacheco, Thábata dos AnjosRamos, Dirceu Guilherme de SouzaSilva, Glaucenyra Cecília Pinheiro daDutra, ValériaAguiar, Daniel MouraPacheco, Richard Campos2022-10-19T13:39:59Zoai:ojs.pkp.sfu.ca:article/33461Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-19T13:39:59Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil Levantamento molecular de Babesia vogeli e Hepatozoon spp. em cães da área urbana do Centro-Oeste do Brasil |
title |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil |
spellingShingle |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil Maia, Maerle Oliveira Canine babesiosis Canine hepatozoonosis PCR Tick-borne diseases. Babesiose canina Hepatozoonose canina PCR Doenças transmitidas por carrapatos. |
title_short |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil |
title_full |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil |
title_fullStr |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil |
title_full_unstemmed |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil |
title_sort |
Molecular survey of Babesia vogeli and Hepatozoon species in dogs from urban area of Midwestern Brazil |
author |
Maia, Maerle Oliveira |
author_facet |
Maia, Maerle Oliveira Freitas, André Luís Santos de Santos, Jamila Guimarães Pacheco, Thábata dos Anjos Ramos, Dirceu Guilherme de Souza Silva, Glaucenyra Cecília Pinheiro da Dutra, Valéria Aguiar, Daniel Moura Pacheco, Richard Campos |
author_role |
author |
author2 |
Freitas, André Luís Santos de Santos, Jamila Guimarães Pacheco, Thábata dos Anjos Ramos, Dirceu Guilherme de Souza Silva, Glaucenyra Cecília Pinheiro da Dutra, Valéria Aguiar, Daniel Moura Pacheco, Richard Campos |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Maia, Maerle Oliveira Freitas, André Luís Santos de Santos, Jamila Guimarães Pacheco, Thábata dos Anjos Ramos, Dirceu Guilherme de Souza Silva, Glaucenyra Cecília Pinheiro da Dutra, Valéria Aguiar, Daniel Moura Pacheco, Richard Campos |
dc.subject.por.fl_str_mv |
Canine babesiosis Canine hepatozoonosis PCR Tick-borne diseases. Babesiose canina Hepatozoonose canina PCR Doenças transmitidas por carrapatos. |
topic |
Canine babesiosis Canine hepatozoonosis PCR Tick-borne diseases. Babesiose canina Hepatozoonose canina PCR Doenças transmitidas por carrapatos. |
description |
In Brazil, the most important tickborne pathogens affecting dogs include Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis. Babesia spp. and Hepatozoon spp., transmitted by ixodid ticks, have been reported to naturally infect dogs and are widespread. The authors aimed to investigate the incidence of B. vogeli and Hepatozoon spp. infection using molecular methods to identify factors associated with the infection in dogs from urban areas of Cuiabá municipality, Midwestern Brazil. Polymerase chain reaction (PCR) assay revealed a prevalence of 9.36% (Confidence Interval-CI 95%; 2.72%; 6.79%) and 9.61% (CI 95%; 7.0%; 13.0%) among dogs for B. vogeli and Hepatozoon, respectively. DNA sequences obtained from 10 Hepatozoon PCR positive samples were sequenced and were identical to one another and, moreover, were 100% (541/541 base of pairs-bp) homologous to the corresponding 18S rDNA sequences of H. canis. Twenty-five dogs (6.02%) generated amplicons using PCR protocols for both organisms, indicating co-infection by these two protozoans. To the best of our knowledge, our study was the first molecular survey to consider the entire population of dogs from the study area. Moreover, young dogs (0-12 months of age), as well as animals living in walled houses?without access to the street?were more susceptible to infection with B. vogeli and H. canis, respectively. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33461 10.5433/1679-0359.2019v40n3p1357 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33461 |
identifier_str_mv |
10.5433/1679-0359.2019v40n3p1357 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33461/25432 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 3 (2019); 1357-1364 Semina: Ciências Agrárias; v. 40 n. 3 (2019); 1357-1364 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306079884017664 |