Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)

Detalhes bibliográficos
Autor(a) principal: Rosa, Janaina Marcela Assunção
Data de Publicação: 2013
Outros Autores: Almeida, Arleana do Bom Parto Ferreira de, Carvalho, Ricardo César Tavares, Brandão, Laila Natasha Santos, Pitchenin, Letícia Camara, Rocha, Icaro Sérgio Magalhães, Sousa, Valéria Régia Franco, Dutra, Valéria, Nakazato, Luciano
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14373
Resumo: The aim of this study was to comparatively evaluate the diagnostic methods of Canine Visceral Leishmaniasis (CVL), using the indirect immunofluorescence assay (IFA), the probe Gold Nanoparticles (AuNPprobes) and PCR in liver samples. We analyzed 25 samples of animals positive by IFA and 10 samples from animals negative to PCR. The probe AUNP used was based on specific sequence for Leishmania infantum kDNA. The results showed that when compared to the IFA sorology the AuNPprobe showed a sensitivity and specificity of 56% and 80%, respectively. When compared to PCR method, the AuNPprobe had a sensitivity of 68% and specificity of 81%. The AuNPprobe can be an alternative to traditional diagnostic methods (PCR and IFA) due to its ease of implementation, immediate result, low cost and little need for laboratory infrastructure.
id UEL-11_c24bd553bd08da149983b071771a60a0
oai_identifier_str oai:ojs.pkp.sfu.ca:article/14373
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)Diagnóstico molecular da leishmaniose visceral canina através da técnica de sonda de nanopartículas de ouro (AuNPprobes)Canine visceral leishmaniasisGold nanoparticlesDiagnosisDogs.Leishmaniose visceral caninaNanopartículas de ouroDiagnósticoCães.Diagnóstico da Leishmaniose Visceral CaninaThe aim of this study was to comparatively evaluate the diagnostic methods of Canine Visceral Leishmaniasis (CVL), using the indirect immunofluorescence assay (IFA), the probe Gold Nanoparticles (AuNPprobes) and PCR in liver samples. We analyzed 25 samples of animals positive by IFA and 10 samples from animals negative to PCR. The probe AUNP used was based on specific sequence for Leishmania infantum kDNA. The results showed that when compared to the IFA sorology the AuNPprobe showed a sensitivity and specificity of 56% and 80%, respectively. When compared to PCR method, the AuNPprobe had a sensitivity of 68% and specificity of 81%. The AuNPprobe can be an alternative to traditional diagnostic methods (PCR and IFA) due to its ease of implementation, immediate result, low cost and little need for laboratory infrastructure.O objetivo da presente pesquisa foi avaliar comparativamente os métodos diagnósticos da Leishmaniose Visceral Canina (LVC), utilizando-se a reação de imunofluorescência indireta (RIFI), a sonda de Nanopartículas de Ouro (AuNPprobes) e a PCR em amostras de fígado. Foram analisadas 25 amostras de animais positivos pela RIFI e 10 amostras de animais negativos à PCR. A AuNPprobes utilizada baseou-se na sequência de kDNA específico para Leishmania infantum. Os resultados demonstraram que ao ser comparada com a RIFI a AuNPprobes apresentou sensibilidade e especificidade de 56% e 80%, respectivamente. Já ao ser comparada à PCR, a AuNPprobes apresentou sensibilidade de 68% e especificidade de 81%. A AuNPprobes pode ser uma alternativa aos métodos de diagnósticos tradicionais (PCR e RIFI), devido a sua facilidade de execução, rapidez, baixo custo e necessidade de pouca infraestrutura laboratorial.UEL2013-12-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1437310.5433/1679-0359.2013v34n6Supl2p3777Semina: Ciências Agrárias; Vol. 34 No. 6Supl2 (2013); 3777-3786Semina: Ciências Agrárias; v. 34 n. 6Supl2 (2013); 3777-37861679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14373/pdf_195Rosa, Janaina Marcela AssunçãoAlmeida, Arleana do Bom Parto Ferreira deCarvalho, Ricardo César TavaresBrandão, Laila Natasha SantosPitchenin, Letícia CamaraRocha, Icaro Sérgio MagalhãesSousa, Valéria Régia FrancoDutra, ValériaNakazato, Lucianoinfo:eu-repo/semantics/openAccess2015-11-19T18:36:05Zoai:ojs.pkp.sfu.ca:article/14373Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:36:05Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
Diagnóstico molecular da leishmaniose visceral canina através da técnica de sonda de nanopartículas de ouro (AuNPprobes)
title Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
spellingShingle Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
Rosa, Janaina Marcela Assunção
Canine visceral leishmaniasis
Gold nanoparticles
Diagnosis
Dogs.
Leishmaniose visceral canina
Nanopartículas de ouro
Diagnóstico
Cães.
Diagnóstico da Leishmaniose Visceral Canina
title_short Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
title_full Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
title_fullStr Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
title_full_unstemmed Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
title_sort Molecular diagnosis of canine visceral leishmaniasis through the technique of probe gold nanoparticles (AuNPprobes)
author Rosa, Janaina Marcela Assunção
author_facet Rosa, Janaina Marcela Assunção
Almeida, Arleana do Bom Parto Ferreira de
Carvalho, Ricardo César Tavares
Brandão, Laila Natasha Santos
Pitchenin, Letícia Camara
Rocha, Icaro Sérgio Magalhães
Sousa, Valéria Régia Franco
Dutra, Valéria
Nakazato, Luciano
author_role author
author2 Almeida, Arleana do Bom Parto Ferreira de
Carvalho, Ricardo César Tavares
Brandão, Laila Natasha Santos
Pitchenin, Letícia Camara
Rocha, Icaro Sérgio Magalhães
Sousa, Valéria Régia Franco
Dutra, Valéria
Nakazato, Luciano
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rosa, Janaina Marcela Assunção
Almeida, Arleana do Bom Parto Ferreira de
Carvalho, Ricardo César Tavares
Brandão, Laila Natasha Santos
Pitchenin, Letícia Camara
Rocha, Icaro Sérgio Magalhães
Sousa, Valéria Régia Franco
Dutra, Valéria
Nakazato, Luciano
dc.subject.por.fl_str_mv Canine visceral leishmaniasis
Gold nanoparticles
Diagnosis
Dogs.
Leishmaniose visceral canina
Nanopartículas de ouro
Diagnóstico
Cães.
Diagnóstico da Leishmaniose Visceral Canina
topic Canine visceral leishmaniasis
Gold nanoparticles
Diagnosis
Dogs.
Leishmaniose visceral canina
Nanopartículas de ouro
Diagnóstico
Cães.
Diagnóstico da Leishmaniose Visceral Canina
description The aim of this study was to comparatively evaluate the diagnostic methods of Canine Visceral Leishmaniasis (CVL), using the indirect immunofluorescence assay (IFA), the probe Gold Nanoparticles (AuNPprobes) and PCR in liver samples. We analyzed 25 samples of animals positive by IFA and 10 samples from animals negative to PCR. The probe AUNP used was based on specific sequence for Leishmania infantum kDNA. The results showed that when compared to the IFA sorology the AuNPprobe showed a sensitivity and specificity of 56% and 80%, respectively. When compared to PCR method, the AuNPprobe had a sensitivity of 68% and specificity of 81%. The AuNPprobe can be an alternative to traditional diagnostic methods (PCR and IFA) due to its ease of implementation, immediate result, low cost and little need for laboratory infrastructure.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-17
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14373
10.5433/1679-0359.2013v34n6Supl2p3777
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14373
identifier_str_mv 10.5433/1679-0359.2013v34n6Supl2p3777
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/14373/pdf_195
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 34 No. 6Supl2 (2013); 3777-3786
Semina: Ciências Agrárias; v. 34 n. 6Supl2 (2013); 3777-3786
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306069097316352

Registros relacionados