Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27466 |
Resumo: | Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis. |
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Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei)Influência das vitaminas C e E sobre a qualidade do semen criopreservado de Prochilodus brevis (Prochilodontidae, Teleostei)AntioxidantsCryopreservation of semenCommon curimatãFish.AntioxidantesCriopreservação seminalCurimatã comumPeixe.Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis.A criopreservação seminal permite a conservação em longo prazo dos gametas de diversas espécies, inclusive as ameaçadas, como a Prochilodus brevis. Contudo, a aplicação dessa biotecnologia pode causar danos às células espermáticas, reduzindo a qualidade seminal. Assim, tem-se buscado substâncias que minimizem os danos causados por esse processo, como os antioxidantes. Deste modo, este estudo objetivou avaliar a associação entre dois crioprotetores e duas vitaminas, em diferentes concentrações, sobre a qualidade do sêmen criopreservado de P. brevis. Para a criopreservação, o experimento foi realizado em duas etapas. Na primeira, o sêmen de 10 animais foi submetido a seis diferentes meios de congelação, oriundos da combinação de glicose 5%, dois crioprotetores (Dimetilsulfóxido [DMSO] ou Metilglicol) e duas vitaminas (C ou E a 0,0001 mg), para a criopreservação. Na segunda etapa, amostras de sêmen de oito animais foram diluídas em Glicose 5% e DMSO (melhor crioprotetor encontrado na primeira etapa), associados a três diferentes concentrações das vitaminas C ou E (0,01; 0,001 e 0,0001 mg). Em ambas as etapas, as amostras in natura e pós-descongeladas foram submetidas às análises de cinética, morfologia e integridade de membrana espermática. O sêmen criopreservado com DMSO apresentou resultados significativamente superiores (p < 0,05) comparado ao congelado com Metilglicol, independente da vitamina utilizada. A taxa de espermatozoides morfologicamente normais foi superior (p < 0,05) nas amostras contendo vitamina, contudo a vitamina E reduziu as taxas de motilidade espermática, independente do crioprotetor utilizado. Quanto às concentrações das vitaminas, obtiveram-se maiores taxas de motilidade quando se criopreservou sêmen com 0,01 e 0,0001 mg de qualquer uma das vitaminas. Entretanto, a maior concentração teve efeito deletério sobre a morfologia espermática de P. brevis. Portanto, a glicose associada ao DMSO e à menor concentração de vitamina C proporciona boa qualidade ao sêmen pós-descongelado de P. brevis.UEL2017-08-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2746610.5433/1679-0359.2017v38n4Supl1p2669Semina: Ciências Agrárias; Vol. 38 No. 4Supl1 (2017); 2669-2680Semina: Ciências Agrárias; v. 38 n. 4Supl1 (2017); 2669-26801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27466/21513Copyright (c) 2017 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessAlmeida-Monteiro, Priscila Silva deAraújo, Mayara Setúbal OliveiraPinheiro, Romulo Roberto RibeiroLopes, Júlia TrugilioFerreira, Yasmim MaiaMontenegro, Assis RubensMaciel, Mônica Aline Parente MeloVanderley, Carminda Sandra Brito Salmito2022-10-21T15:55:23Zoai:ojs.pkp.sfu.ca:article/27466Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-21T15:55:23Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) Influência das vitaminas C e E sobre a qualidade do semen criopreservado de Prochilodus brevis (Prochilodontidae, Teleostei) |
title |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) |
spellingShingle |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) Almeida-Monteiro, Priscila Silva de Antioxidants Cryopreservation of semen Common curimatã Fish. Antioxidantes Criopreservação seminal Curimatã comum Peixe. |
title_short |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) |
title_full |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) |
title_fullStr |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) |
title_full_unstemmed |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) |
title_sort |
Influence of vitamins C and E on the quality of cryopreserved semen Prochilodus brevis (Prochilodontidae, Teleostei) |
author |
Almeida-Monteiro, Priscila Silva de |
author_facet |
Almeida-Monteiro, Priscila Silva de Araújo, Mayara Setúbal Oliveira Pinheiro, Romulo Roberto Ribeiro Lopes, Júlia Trugilio Ferreira, Yasmim Maia Montenegro, Assis Rubens Maciel, Mônica Aline Parente Melo Vanderley, Carminda Sandra Brito Salmito |
author_role |
author |
author2 |
Araújo, Mayara Setúbal Oliveira Pinheiro, Romulo Roberto Ribeiro Lopes, Júlia Trugilio Ferreira, Yasmim Maia Montenegro, Assis Rubens Maciel, Mônica Aline Parente Melo Vanderley, Carminda Sandra Brito Salmito |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Almeida-Monteiro, Priscila Silva de Araújo, Mayara Setúbal Oliveira Pinheiro, Romulo Roberto Ribeiro Lopes, Júlia Trugilio Ferreira, Yasmim Maia Montenegro, Assis Rubens Maciel, Mônica Aline Parente Melo Vanderley, Carminda Sandra Brito Salmito |
dc.subject.por.fl_str_mv |
Antioxidants Cryopreservation of semen Common curimatã Fish. Antioxidantes Criopreservação seminal Curimatã comum Peixe. |
topic |
Antioxidants Cryopreservation of semen Common curimatã Fish. Antioxidantes Criopreservação seminal Curimatã comum Peixe. |
description |
Seminal cryopreservation allows the long-term conservation of gametes of various species, including endangered species, such as Prochilodus brevis. However, the application of this biotechnology can cause damage to sperm cells, reducing seminal quality. Thus, we have sought substances that minimize the damage caused by this process, such as antioxidants. Thus, this study aimed to evaluate the association between two cryoprotectants and two vitamins, in different concentrations, on the quality of cryopreserved semen of P. brevis. For cryopreservation, the experiment was performed in two stages. In the first stage, the semen of 10 animals was submitted to six different freezing means, coming from the combination of 5% glucose, two cryoprotectants (Dimethyl sulfoxide [DMSO] or Methyl glycol) and two vitamins (C or E to 0.0001 mg) for cryopreservation. In the second stage, semen samples of eight animals were diluted in 5% glucose and the best cryoprotectant found in the first stage, associated with three different concentrations of vitamins C or E (0.01, 0.001, and 0.0001 mg). In both steps, the in natura and post-thawed samples were submitted to kinetic analysis, morphology, and sperm membrane integrity. The cryopreserved semen with DMSO presented significantly higher results (p < 0.05) than that frozen with Methyl glycol, regardless of the vitamin used. The morphologically normal spermatozoa rate was higher (p < 0.05) in the vitamin-containing samples, however, vitamin E reduced sperm motility rates, independent of the cryoprotectant used. As for vitamin concentrations, higher motility rates were obtained when cryopreserved semen with 0.01 and 0.0001 mg of any of the vitamins. However, the higher concentration had a deleterious effect on the spermatic morphology of P. brevis. Therefore, the glucose associated with DMSO and the lower concentration of vitamin C provides good quality for the post-thawed semen of P. brevis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-08-25 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27466 10.5433/1679-0359.2017v38n4Supl1p2669 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27466 |
identifier_str_mv |
10.5433/1679-0359.2017v38n4Supl1p2669 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/27466/21513 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 38 No. 4Supl1 (2017); 2669-2680 Semina: Ciências Agrárias; v. 38 n. 4Supl1 (2017); 2669-2680 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306076998336512 |