Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl

Detalhes bibliográficos
Autor(a) principal: Carvalho, Elck Almeida
Data de Publicação: 2012
Outros Autores: Melo Neto, Biano Alves de, Holschuh, Heinz Johann, Franco, Marcelo, Sacramento, Célio Kersul do
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11000
Resumo: The objective was to obtain and characterize pectinmethylesterase (PME) of industrial residue from the Pearl Pineapple. Specific activities, the optimum conditions of pH and temperature, and also the thermal stability partially purified and concentrated PME were determined. The PME specific activity was 6.36 U/mg. The pH optimum of 7.5 PME was extracted, also considering alkaline. During the trial time, the partially purified PME maintained itself stable upon exposure to the temperatures of 25 and 60ºC. It obtained an activity elevation between 40 and 50ºC. In temperatures above 70ºC, there was a substantial reduction in a specific activity. The partially purified PME showed three gel polyacrylamide SDS-PAGE bands of apparent molar mass (MM) of 25.4 and 23.1 kDa and 16.4 kDa. These results demonstrate the feasibility of PME extraction from the Pearl Pineapple’s processed residue and its potential for use in manufacturing pulp and fruit juices, since the currently applied enzymes are imported and expensive.
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spelling Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearlExtração e caracterização de pectinametilesterase (PME) de resíduos agroindustrais de abacaxi pérolaPurificationPectinmethylesterasePineappleAgro-industrial waste.PurificaçãoPectinametilesteraseAbacaxiResíduo agroindustrial.Ciência e Tecnologia de AlimentosThe objective was to obtain and characterize pectinmethylesterase (PME) of industrial residue from the Pearl Pineapple. Specific activities, the optimum conditions of pH and temperature, and also the thermal stability partially purified and concentrated PME were determined. The PME specific activity was 6.36 U/mg. The pH optimum of 7.5 PME was extracted, also considering alkaline. During the trial time, the partially purified PME maintained itself stable upon exposure to the temperatures of 25 and 60ºC. It obtained an activity elevation between 40 and 50ºC. In temperatures above 70ºC, there was a substantial reduction in a specific activity. The partially purified PME showed three gel polyacrylamide SDS-PAGE bands of apparent molar mass (MM) of 25.4 and 23.1 kDa and 16.4 kDa. These results demonstrate the feasibility of PME extraction from the Pearl Pineapple’s processed residue and its potential for use in manufacturing pulp and fruit juices, since the currently applied enzymes are imported and expensive.Objetivou obter e caracterizar pectinametilesterase (PME) de resíduos industriais de abacaxi Pérola. Foram determinadas a atividade específica, as condições ótimas de pH e temperatura, bem como a estabilidade térmica da PME concentrada (PME-C) e PME parcialmente purificada (PME-PP). A atividade específica da PME foi de 6,36 U/MG. O pH ótimo da PME extraída foi 8,5, classificada como alcalina. Durante o tempo de ensaio, a PME-PP manteve-se estável mediante exposição a temperaturas entre 25 e 60°C, obtendo uma elevação da atividade em 40 e 50°C. Em temperaturas superiores a 70°C, houve uma redução na atividade específica. A PME-PP mostrou três bandas no gel poliacrilamida SDS-PAGE de massas molares aparentes (MM) de 25,4 e 23,1 kDa e 16,4 kDa. Os resultados obtidos demonstram ser possível explorar o potencial de uso da PME extraída a partir do subproduto do processamento de abacaxi Pérola. Esta enzima está presente em praticamente todas as preparações enzimáticas comerciais para proteção e melhoramento da textura e firmeza de frutas e vegetais processados, na extração e clarificação de sucos de frutas. Atualmente, são importadas e tem elevado custo.UEL2012-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPequisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1100010.5433/1679-0359.2012v33n6p2257Semina: Ciências Agrárias; Vol. 33 No. 6 (2012); 2257-2266Semina: Ciências Agrárias; v. 33 n. 6 (2012); 2257-22661679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11000/11682Copyright (c) 2012 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCarvalho, Elck AlmeidaMelo Neto, Biano Alves deHolschuh, Heinz JohannFranco, MarceloSacramento, Célio Kersul do2023-01-27T13:07:07Zoai:ojs.pkp.sfu.ca:article/11000Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-27T13:07:07Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
Extração e caracterização de pectinametilesterase (PME) de resíduos agroindustrais de abacaxi pérola
title Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
spellingShingle Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
Carvalho, Elck Almeida
Purification
Pectinmethylesterase
Pineapple
Agro-industrial waste.
Purificação
Pectinametilesterase
Abacaxi
Resíduo agroindustrial.
Ciência e Tecnologia de Alimentos
title_short Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
title_full Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
title_fullStr Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
title_full_unstemmed Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
title_sort Extraction and characterization of the pectin methylesterase (PME) of waste agroindustrial of the pineapple pearl
author Carvalho, Elck Almeida
author_facet Carvalho, Elck Almeida
Melo Neto, Biano Alves de
Holschuh, Heinz Johann
Franco, Marcelo
Sacramento, Célio Kersul do
author_role author
author2 Melo Neto, Biano Alves de
Holschuh, Heinz Johann
Franco, Marcelo
Sacramento, Célio Kersul do
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Elck Almeida
Melo Neto, Biano Alves de
Holschuh, Heinz Johann
Franco, Marcelo
Sacramento, Célio Kersul do
dc.subject.por.fl_str_mv Purification
Pectinmethylesterase
Pineapple
Agro-industrial waste.
Purificação
Pectinametilesterase
Abacaxi
Resíduo agroindustrial.
Ciência e Tecnologia de Alimentos
topic Purification
Pectinmethylesterase
Pineapple
Agro-industrial waste.
Purificação
Pectinametilesterase
Abacaxi
Resíduo agroindustrial.
Ciência e Tecnologia de Alimentos
description The objective was to obtain and characterize pectinmethylesterase (PME) of industrial residue from the Pearl Pineapple. Specific activities, the optimum conditions of pH and temperature, and also the thermal stability partially purified and concentrated PME were determined. The PME specific activity was 6.36 U/mg. The pH optimum of 7.5 PME was extracted, also considering alkaline. During the trial time, the partially purified PME maintained itself stable upon exposure to the temperatures of 25 and 60ºC. It obtained an activity elevation between 40 and 50ºC. In temperatures above 70ºC, there was a substantial reduction in a specific activity. The partially purified PME showed three gel polyacrylamide SDS-PAGE bands of apparent molar mass (MM) of 25.4 and 23.1 kDa and 16.4 kDa. These results demonstrate the feasibility of PME extraction from the Pearl Pineapple’s processed residue and its potential for use in manufacturing pulp and fruit juices, since the currently applied enzymes are imported and expensive.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pequisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11000
10.5433/1679-0359.2012v33n6p2257
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11000
identifier_str_mv 10.5433/1679-0359.2012v33n6p2257
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11000/11682
dc.rights.driver.fl_str_mv Copyright (c) 2012 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2012 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 33 No. 6 (2012); 2257-2266
Semina: Ciências Agrárias; v. 33 n. 6 (2012); 2257-2266
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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