Diurnal variations in canine hematological parameters after commercial feed feeding
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307 |
Resumo: | Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process. |
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Semina. Ciências Agrárias (Online) |
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Diurnal variations in canine hematological parameters after commercial feed feedingVariações diurnas nos parâmetros hematológicos caninos após alimentação com ração comercialComplete blood countDogFood.HemogramaCãoAlimento.Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process.O hemograma é a avaliação das células do sangue e fornece grande diversidade de informações. Considerando que o tempo inadequado de jejum é o erro pré-analítico mais comum no diagnóstico laboratorial por causar lipemia nas amostras sanguíneas e tal interferência ainda não foi adequadamente investigada no hemograma de cães, esse trabalho teve como objetivo analisar as alterações pós-prandiais diurnas no hemograma de cães saudáveis alimentados com ração comercial. Foram selecionados 18 cães clinicamente saudáveis, entre 2 e 6 anos, pesando mais de 6 kg e que recebiam como alimentação ração comercial tipo “Premium” a cada 12 horas. O sangue dos animais foi colhido após jejum de 12 horas às 6:00 da manhã (amostra basal), foi fornecida ração comercial e novas amostras foram colhidas nas 11 horas consecutivas. As contagens de hemácias, amplitude de distribuição eritrocitária (RDW), leucócitos totais, plaquetas, volume plaquetário médio (VPM) e hemoglobina foram realizadas em contador automatizado de células veterinário. O volume globular (VG) foi determinado pelo método do microcapilar de Strumia, a contagem diferencial de leucócitos foi realizada em esfregaço sanguíneo corado com corante hematológico e a proteína plasmática total (PPT) foi determinada por refratometria. As variáveis foram testadas quanto à normalidade e as diferenças foram consideradas significativas quando p < 0,05. Em relação ao eritrograma, houve redução estatisticamente significativa a partir de 2 h para hemácias e hemoglobina, a partir de 3 h para o VG e a partir de 4 h para o VCM, persistindo até o final das 11 horas. Não houve alteração do CHCM e do RDW. Em relação ao leucograma, foi observado aumento significativo de leucócitos totais de 2 a 7 h, decorrente do aumento de neutrófilos segmentados, o que foi constatado 2 a 8 h após a alimentação. O valor de linfócitos reduziu significativamente nos momentos 2 e 6 h após a alimentação. Não foi observada alteração nas contagens de eosinófilos, basófilos, monócitos e plaquetas, bem como no VPM. Os níveis de PPT reduziram significativamente de 5 h até 11 h após a alimentação. Contudo, tais alterações nos parâmetros hematológicos não excederam os valores de referência para a espécie canina. A alimentação com ração comercial causa alterações estatisticamente significativas no eritrograma, leucograma e teor de proteína plasmática em cães. Para animais saudáveis, tais alterações parecem não exceder valores de referência para espécie, porém para animais enfermos com valores limítrofes, tais alterações poderiam ser clinicamente importantes dependendo da patologia envolvida.UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3830710.5433/1679-0359.2020v41n5supl1p2219Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2219-2230Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2219-22301679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307/27800Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCosta, Letícia RamosSilva, Nathalia Lopes Tavares daOliveira, Paula Lima deBonatto, Natalia Camila MinucciVieira, Guilherme CoutinhoFloriano, Beatriz PerezBarros, Luiz Daniel deBosculo, Maria Rachel MeloAlmeida, Breno Fernando Martins de2022-10-07T13:46:38Zoai:ojs.pkp.sfu.ca:article/38307Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T13:46:38Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Diurnal variations in canine hematological parameters after commercial feed feeding Variações diurnas nos parâmetros hematológicos caninos após alimentação com ração comercial |
title |
Diurnal variations in canine hematological parameters after commercial feed feeding |
spellingShingle |
Diurnal variations in canine hematological parameters after commercial feed feeding Costa, Letícia Ramos Complete blood count Dog Food. Hemograma Cão Alimento. |
title_short |
Diurnal variations in canine hematological parameters after commercial feed feeding |
title_full |
Diurnal variations in canine hematological parameters after commercial feed feeding |
title_fullStr |
Diurnal variations in canine hematological parameters after commercial feed feeding |
title_full_unstemmed |
Diurnal variations in canine hematological parameters after commercial feed feeding |
title_sort |
Diurnal variations in canine hematological parameters after commercial feed feeding |
author |
Costa, Letícia Ramos |
author_facet |
Costa, Letícia Ramos Silva, Nathalia Lopes Tavares da Oliveira, Paula Lima de Bonatto, Natalia Camila Minucci Vieira, Guilherme Coutinho Floriano, Beatriz Perez Barros, Luiz Daniel de Bosculo, Maria Rachel Melo Almeida, Breno Fernando Martins de |
author_role |
author |
author2 |
Silva, Nathalia Lopes Tavares da Oliveira, Paula Lima de Bonatto, Natalia Camila Minucci Vieira, Guilherme Coutinho Floriano, Beatriz Perez Barros, Luiz Daniel de Bosculo, Maria Rachel Melo Almeida, Breno Fernando Martins de |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Letícia Ramos Silva, Nathalia Lopes Tavares da Oliveira, Paula Lima de Bonatto, Natalia Camila Minucci Vieira, Guilherme Coutinho Floriano, Beatriz Perez Barros, Luiz Daniel de Bosculo, Maria Rachel Melo Almeida, Breno Fernando Martins de |
dc.subject.por.fl_str_mv |
Complete blood count Dog Food. Hemograma Cão Alimento. |
topic |
Complete blood count Dog Food. Hemograma Cão Alimento. |
description |
Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-08-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307 10.5433/1679-0359.2020v41n5supl1p2219 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307 |
identifier_str_mv |
10.5433/1679-0359.2020v41n5supl1p2219 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307/27800 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2219-2230 Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2219-2230 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306082277916672 |