Diurnal variations in canine hematological parameters after commercial feed feeding

Detalhes bibliográficos
Autor(a) principal: Costa, Letícia Ramos
Data de Publicação: 2020
Outros Autores: Silva, Nathalia Lopes Tavares da, Oliveira, Paula Lima de, Bonatto, Natalia Camila Minucci, Vieira, Guilherme Coutinho, Floriano, Beatriz Perez, Barros, Luiz Daniel de, Bosculo, Maria Rachel Melo, Almeida, Breno Fernando Martins de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307
Resumo: Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process.
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spelling Diurnal variations in canine hematological parameters after commercial feed feedingVariações diurnas nos parâmetros hematológicos caninos após alimentação com ração comercialComplete blood countDogFood.HemogramaCãoAlimento.Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process.O hemograma é a avaliação das células do sangue e fornece grande diversidade de informações. Considerando que o tempo inadequado de jejum é o erro pré-analítico mais comum no diagnóstico laboratorial por causar lipemia nas amostras sanguíneas e tal interferência ainda não foi adequadamente investigada no hemograma de cães, esse trabalho teve como objetivo analisar as alterações pós-prandiais diurnas no hemograma de cães saudáveis alimentados com ração comercial. Foram selecionados 18 cães clinicamente saudáveis, entre 2 e 6 anos, pesando mais de 6 kg e que recebiam como alimentação ração comercial tipo “Premium” a cada 12 horas. O sangue dos animais foi colhido após jejum de 12 horas às 6:00 da manhã (amostra basal), foi fornecida ração comercial e novas amostras foram colhidas nas 11 horas consecutivas. As contagens de hemácias, amplitude de distribuição eritrocitária (RDW), leucócitos totais, plaquetas, volume plaquetário médio (VPM) e hemoglobina foram realizadas em contador automatizado de células veterinário. O volume globular (VG) foi determinado pelo método do microcapilar de Strumia, a contagem diferencial de leucócitos foi realizada em esfregaço sanguíneo corado com corante hematológico e a proteína plasmática total (PPT) foi determinada por refratometria. As variáveis foram testadas quanto à normalidade e as diferenças foram consideradas significativas quando p < 0,05. Em relação ao eritrograma, houve redução estatisticamente significativa a partir de 2 h para hemácias e hemoglobina, a partir de 3 h para o VG e a partir de 4 h para o VCM, persistindo até o final das 11 horas. Não houve alteração do CHCM e do RDW. Em relação ao leucograma, foi observado aumento significativo de leucócitos totais de 2 a 7 h, decorrente do aumento de neutrófilos segmentados, o que foi constatado 2 a 8 h após a alimentação. O valor de linfócitos reduziu significativamente nos momentos 2 e 6 h após a alimentação. Não foi observada alteração nas contagens de eosinófilos, basófilos, monócitos e plaquetas, bem como no VPM. Os níveis de PPT reduziram significativamente de 5 h até 11 h após a alimentação. Contudo, tais alterações nos parâmetros hematológicos não excederam os valores de referência para a espécie canina. A alimentação com ração comercial causa alterações estatisticamente significativas no eritrograma, leucograma e teor de proteína plasmática em cães. Para animais saudáveis, tais alterações parecem não exceder valores de referência para espécie, porém para animais enfermos com valores limítrofes, tais alterações poderiam ser clinicamente importantes dependendo da patologia envolvida.UEL2020-08-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3830710.5433/1679-0359.2020v41n5supl1p2219Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2219-2230Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2219-22301679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307/27800Copyright (c) 2020 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessCosta, Letícia RamosSilva, Nathalia Lopes Tavares daOliveira, Paula Lima deBonatto, Natalia Camila MinucciVieira, Guilherme CoutinhoFloriano, Beatriz PerezBarros, Luiz Daniel deBosculo, Maria Rachel MeloAlmeida, Breno Fernando Martins de2022-10-07T13:46:38Zoai:ojs.pkp.sfu.ca:article/38307Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-07T13:46:38Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Diurnal variations in canine hematological parameters after commercial feed feeding
Variações diurnas nos parâmetros hematológicos caninos após alimentação com ração comercial
title Diurnal variations in canine hematological parameters after commercial feed feeding
spellingShingle Diurnal variations in canine hematological parameters after commercial feed feeding
Costa, Letícia Ramos
Complete blood count
Dog
Food.
Hemograma
Cão
Alimento.
title_short Diurnal variations in canine hematological parameters after commercial feed feeding
title_full Diurnal variations in canine hematological parameters after commercial feed feeding
title_fullStr Diurnal variations in canine hematological parameters after commercial feed feeding
title_full_unstemmed Diurnal variations in canine hematological parameters after commercial feed feeding
title_sort Diurnal variations in canine hematological parameters after commercial feed feeding
author Costa, Letícia Ramos
author_facet Costa, Letícia Ramos
Silva, Nathalia Lopes Tavares da
Oliveira, Paula Lima de
Bonatto, Natalia Camila Minucci
Vieira, Guilherme Coutinho
Floriano, Beatriz Perez
Barros, Luiz Daniel de
Bosculo, Maria Rachel Melo
Almeida, Breno Fernando Martins de
author_role author
author2 Silva, Nathalia Lopes Tavares da
Oliveira, Paula Lima de
Bonatto, Natalia Camila Minucci
Vieira, Guilherme Coutinho
Floriano, Beatriz Perez
Barros, Luiz Daniel de
Bosculo, Maria Rachel Melo
Almeida, Breno Fernando Martins de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Letícia Ramos
Silva, Nathalia Lopes Tavares da
Oliveira, Paula Lima de
Bonatto, Natalia Camila Minucci
Vieira, Guilherme Coutinho
Floriano, Beatriz Perez
Barros, Luiz Daniel de
Bosculo, Maria Rachel Melo
Almeida, Breno Fernando Martins de
dc.subject.por.fl_str_mv Complete blood count
Dog
Food.
Hemograma
Cão
Alimento.
topic Complete blood count
Dog
Food.
Hemograma
Cão
Alimento.
description Complete blood count (CBC) is the evaluation of blood cells, which provides resourceful information. Considering that inadequate fasting time is the most common pre-analytical error in laboratory diagnosis and is associated with lipemia in blood samples, which has not yet been adequately investigated in dogs, this study aimed to assess diurnal postprandial changes in the CBC of healthy dogs fed with industrialized feed. Eighteen clinically healthy dogs aged 2-6 years and weighing over 6 kg were enrolled in the study. All dogs received “Premium” industrialized feed every 12 hours. Blood was collected after a 12-hour fasting period at 6:00 am (baseline), followed by feeding and new blood samples collected hourly for the next 11 consecutive hours. Red blood cell (RBC) counts, red cell distribution width (RDW), white blood cell (WBC) counts, platelets, mean platelet volume (MPV) and hemoglobin were obtained using an automated veterinary cell counter. Hematocrit (HTC) was determined by Strumia's microcapillary method, differential leukocyte count was performed on hematological dye-stained blood smears and total plasma protein (TPP) was determined using refractometry. Variables were tested for normality and differences were considered significant when p < 0.05. A statistically significant decrease was observed in the erythrogram from 2 h for RBC and hemoglobin, from 3 h for HTC and from 4 h for MCV, persisting until the end of 11 hours. There was no change in MCHC and RDW. Regarding the leukogram, a significant increase in WBC was observed from 2 to 7 h, due to the increase in segmented neutrophils 2 to 8 h following feeding. Lymphocyte counts decreased significantly at 2 and 6 h following feeding. No alteration was observed in eosinophil, basophil, monocyte and platelet counts, as well as in MPV. From 5 h to 11 h after feeding, a significant decrease was seen on TPP. However, changes in hematological parameters did not exceed reference ranges for the canine species. Feeding dogs with industrialized feed caused statistically significant changes in erythrogram, leukogram and plasma protein content. While these changes do not seem to exceed reference values for the species in healthy animals, caution is warranted for sick animals with borderline values, in which these changes might be clinically important depending on the pathologic process.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-07
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307
10.5433/1679-0359.2020v41n5supl1p2219
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307
identifier_str_mv 10.5433/1679-0359.2020v41n5supl1p2219
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/38307/27800
dc.rights.driver.fl_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2020 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 41 No. 5supl1 (2020); 2219-2230
Semina: Ciências Agrárias; v. 41 n. 5supl1 (2020); 2219-2230
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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