Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11071 |
Resumo: | This study aims to evaluate the atypical lymphocyte in Holstein dairy cows in seropositive animals for bovine leukemia virus (BLV) with or without persistent lymphocytosis and in seronegative BLV ones, and correlate them with apoptosis of cells CD5+ and lymphocyte proliferation. Thus, we selected 56 animals, and divided according to the white blood count and serological response for BLV performed by agar gel immunodiffusion and by enzyme immunoabsorbent assay in: negative (N, n=25), nonlymphocytic (AL, n=12) and with persistent lymphocytosis (PL, n=19). In these animals, the atypical lymphocyte was evaluated. 15 animals were selected, five for each group, and they were evaluated for correlation about atypical lymphocyte and lymphocyte proliferation or apoptosis of CD5+ cells. The results of this study showed that the absolute and relative values of atypical lymphocytes were higher in group PL. Regarding atypical lymphocytes, lymphocytes Gumprecht shadow, lymphocyte and monocytoid lymphocytes with dual core, were found in the three study groups, but more commonly found in animals infected with BLV especially in animals with PL. The apoptosis of CD5+ cells has a positive correlation with lymphocytes with dual core, which did not occur in other cell populations. The negative correlation between lymphocyte proliferation and monocytoid lymphocytes was observed, which did not occur in other cell populations. Therefore, the animals naturally infected BLV with PL may be associated with increased atypical lymphocytes of the type of shadow Gumprecht, lymphocyte and lymphocyte monocytoid dual-core. |
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Semina. Ciências Agrárias (Online) |
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Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cowsCorrelação entre a atipia linfocitária e o perfil imunológico de vacas leiteiras infectadas pelo vírus da leucemia bovinaLymphocyteCattlePersistent lymphocytosisApoptosisCell proliferation.LinfócitosBovinosLinfocitose persistenteApoptoseProliferação celular.Clinica VeterináriaThis study aims to evaluate the atypical lymphocyte in Holstein dairy cows in seropositive animals for bovine leukemia virus (BLV) with or without persistent lymphocytosis and in seronegative BLV ones, and correlate them with apoptosis of cells CD5+ and lymphocyte proliferation. Thus, we selected 56 animals, and divided according to the white blood count and serological response for BLV performed by agar gel immunodiffusion and by enzyme immunoabsorbent assay in: negative (N, n=25), nonlymphocytic (AL, n=12) and with persistent lymphocytosis (PL, n=19). In these animals, the atypical lymphocyte was evaluated. 15 animals were selected, five for each group, and they were evaluated for correlation about atypical lymphocyte and lymphocyte proliferation or apoptosis of CD5+ cells. The results of this study showed that the absolute and relative values of atypical lymphocytes were higher in group PL. Regarding atypical lymphocytes, lymphocytes Gumprecht shadow, lymphocyte and monocytoid lymphocytes with dual core, were found in the three study groups, but more commonly found in animals infected with BLV especially in animals with PL. The apoptosis of CD5+ cells has a positive correlation with lymphocytes with dual core, which did not occur in other cell populations. The negative correlation between lymphocyte proliferation and monocytoid lymphocytes was observed, which did not occur in other cell populations. Therefore, the animals naturally infected BLV with PL may be associated with increased atypical lymphocytes of the type of shadow Gumprecht, lymphocyte and lymphocyte monocytoid dual-core.O presente trabalho teve o objetivo avaliar a atipia linfocitária, em bovinos da raça Holandesa Preto e Branco naturalmente infectados pelo vírus da leucemia bovina (BLV) correlacionando-a com as duas formas de manifestação da doença, linfocitose persistente (LP) e alinfocitóticos (AL). Assim, foram selecionados 56 animais, que foram divididos em três grupos distintos, sendo eles negativos (N, n=25), alinfocitóticos ( n=12) e com linfocitose persistente (n=19), de acordo com resultados do leucograma e do sorodiagnóstico da Leucose Enzoótica Bovina (LEB) pela imunodifusão em agar gel e pelo ensaio imunoenzimático. Destes, foram avaliadas as atipias linfocitárias. Em 15 animais, cinco de cada grupo, realizou-se análise de correlação entre as atipias linfocitárias e com os índices de proliferação de linfócitos e apoptose de células CD5+. Os resultados deste estudo mostraram que os linfócitos atípicos, e suas diferentes morfologias: sombra de Gümprecht, linfócito monocitóide e linfócito com núcleo duplo, foram encontrados nos três grupos, porém mais comumente em animais infectados pelo BLV, predominantemente no grupo manifestando LP. Na análise da correlação entre a apoptose de células CD5+ e a porcentagem das populações linfocitárias, observou-se que a apoptose teve correlação negativa com atipia linfocitária. Na correlação entre a proliferação, observou-se que quanto maior a porcentagem de linfócitos, e de linfócitos monocitóides, menor é a proliferação, o que não ocorreu nas demais populações celulares. Portanto, a manifestação da LP em animais naturalmente infectados pelo BLV, pode ser associada ao aumento de linfócitos atípicos, principalmente os do tipo sombra de Gümprecht, linfócito monocitóide e linfócito núcleo duplo.UEL2013-03-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1107110.5433/1679-0359.2013v34n1p293Semina: Ciências Agrárias; Vol. 34 No. 1 (2013); 293-300Semina: Ciências Agrárias; v. 34 n. 1 (2013); 293-3001679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11071/pdfCopyright (c) 2013 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSpinola, Tatiana RezendeBertagnon, Heloisa GodoiBatista, Camila FreitasSouza, Fernando NogueiraAzedo, Milton RicardoBlagitz, Maiara GarciaBenesi, Fernando JoséDella Libera, Alice Maria Melville Paiva2023-01-26T14:35:39Zoai:ojs.pkp.sfu.ca:article/11071Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-26T14:35:39Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows Correlação entre a atipia linfocitária e o perfil imunológico de vacas leiteiras infectadas pelo vírus da leucemia bovina |
title |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows |
spellingShingle |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows Spinola, Tatiana Rezende Lymphocyte Cattle Persistent lymphocytosis Apoptosis Cell proliferation. Linfócitos Bovinos Linfocitose persistente Apoptose Proliferação celular. Clinica Veterinária |
title_short |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows |
title_full |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows |
title_fullStr |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows |
title_full_unstemmed |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows |
title_sort |
Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows |
author |
Spinola, Tatiana Rezende |
author_facet |
Spinola, Tatiana Rezende Bertagnon, Heloisa Godoi Batista, Camila Freitas Souza, Fernando Nogueira Azedo, Milton Ricardo Blagitz, Maiara Garcia Benesi, Fernando José Della Libera, Alice Maria Melville Paiva |
author_role |
author |
author2 |
Bertagnon, Heloisa Godoi Batista, Camila Freitas Souza, Fernando Nogueira Azedo, Milton Ricardo Blagitz, Maiara Garcia Benesi, Fernando José Della Libera, Alice Maria Melville Paiva |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Spinola, Tatiana Rezende Bertagnon, Heloisa Godoi Batista, Camila Freitas Souza, Fernando Nogueira Azedo, Milton Ricardo Blagitz, Maiara Garcia Benesi, Fernando José Della Libera, Alice Maria Melville Paiva |
dc.subject.por.fl_str_mv |
Lymphocyte Cattle Persistent lymphocytosis Apoptosis Cell proliferation. Linfócitos Bovinos Linfocitose persistente Apoptose Proliferação celular. Clinica Veterinária |
topic |
Lymphocyte Cattle Persistent lymphocytosis Apoptosis Cell proliferation. Linfócitos Bovinos Linfocitose persistente Apoptose Proliferação celular. Clinica Veterinária |
description |
This study aims to evaluate the atypical lymphocyte in Holstein dairy cows in seropositive animals for bovine leukemia virus (BLV) with or without persistent lymphocytosis and in seronegative BLV ones, and correlate them with apoptosis of cells CD5+ and lymphocyte proliferation. Thus, we selected 56 animals, and divided according to the white blood count and serological response for BLV performed by agar gel immunodiffusion and by enzyme immunoabsorbent assay in: negative (N, n=25), nonlymphocytic (AL, n=12) and with persistent lymphocytosis (PL, n=19). In these animals, the atypical lymphocyte was evaluated. 15 animals were selected, five for each group, and they were evaluated for correlation about atypical lymphocyte and lymphocyte proliferation or apoptosis of CD5+ cells. The results of this study showed that the absolute and relative values of atypical lymphocytes were higher in group PL. Regarding atypical lymphocytes, lymphocytes Gumprecht shadow, lymphocyte and monocytoid lymphocytes with dual core, were found in the three study groups, but more commonly found in animals infected with BLV especially in animals with PL. The apoptosis of CD5+ cells has a positive correlation with lymphocytes with dual core, which did not occur in other cell populations. The negative correlation between lymphocyte proliferation and monocytoid lymphocytes was observed, which did not occur in other cell populations. Therefore, the animals naturally infected BLV with PL may be associated with increased atypical lymphocytes of the type of shadow Gumprecht, lymphocyte and lymphocyte monocytoid dual-core. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11071 10.5433/1679-0359.2013v34n1p293 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11071 |
identifier_str_mv |
10.5433/1679-0359.2013v34n1p293 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/11071/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 34 No. 1 (2013); 293-300 Semina: Ciências Agrárias; v. 34 n. 1 (2013); 293-300 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306066563956736 |