In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20106 |
Resumo: | The control of nematodes in plants can be challenging, and there is a need for alternative, environmentally conscious methods for their management. The purpose of this study was to evaluate the effect of rosemary extract (Rosmarinus officinalis) on the in vitro toxicity and control of Meloidogyne incognita in CD 206 and CD 215 soybean cultivars. Using an in vitro assay, 500 M. incognita eggs per plate were observed for 15 days after incubation with rosemary extract at concentrations of 1%, 5%, and 10%. Soybean plants were studied under greenhouse conditions, and starting at V3 stage, were sprayed weekly with the same concentration of rosemary extract for 64 days. Three days after the first treatment, each soybean plant was inoculated with 1800 eggs and 400 second-stage juveniles (J2). At the end of this essay, number of eggs and J2 in the roots and soil, number of galls, and the reproduction factor (RF) were evaluated. Our results showed that in the in vitro assay, rosemary extract reduced the number of M. incognita eggs that hatched. Under greenhouse conditions, the CD 206 cultivar showed a 48% reduction in the number of galls, as well as fewer eggs in the soil and a lower RF. Similarly, in the CD 215 cultivar, the number of eggs was reduced and the RF was lower. These results indicate the potential for rosemary extract to control M. incognita in soybean crops. |
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In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extractToxicidade in vitro e controle de Meloidogyne incognita em soja por extrato aquoso de alecrimAlternative controlResistance inductionRoot-knot nematodeRosemary extract.Controle alternativoIndução de resistênciaNematoide de galhaRosmarinus officinalis.The control of nematodes in plants can be challenging, and there is a need for alternative, environmentally conscious methods for their management. The purpose of this study was to evaluate the effect of rosemary extract (Rosmarinus officinalis) on the in vitro toxicity and control of Meloidogyne incognita in CD 206 and CD 215 soybean cultivars. Using an in vitro assay, 500 M. incognita eggs per plate were observed for 15 days after incubation with rosemary extract at concentrations of 1%, 5%, and 10%. Soybean plants were studied under greenhouse conditions, and starting at V3 stage, were sprayed weekly with the same concentration of rosemary extract for 64 days. Three days after the first treatment, each soybean plant was inoculated with 1800 eggs and 400 second-stage juveniles (J2). At the end of this essay, number of eggs and J2 in the roots and soil, number of galls, and the reproduction factor (RF) were evaluated. Our results showed that in the in vitro assay, rosemary extract reduced the number of M. incognita eggs that hatched. Under greenhouse conditions, the CD 206 cultivar showed a 48% reduction in the number of galls, as well as fewer eggs in the soil and a lower RF. Similarly, in the CD 215 cultivar, the number of eggs was reduced and the RF was lower. These results indicate the potential for rosemary extract to control M. incognita in soybean crops. Devido à dificuldade de manejo de nematoides e a necessidade de desenvolver métodos alternativos de controle, objetivou-se avaliar a toxicidade in vitro do extrato aquoso de alecrim (Rosmarinus officinalis), assim como sua capacidade de controle de Meloidogyne incognita nos genótipos de soja CD206 e CD215. Em ensaio in vitro, para avaliar a capacidade inibitória de eclosão de juvenis, depositou-se 500 ovos/placa quantificando o número de juvenis eclodidos após 15 dias de incubação com 1%, 5% e 10% do extrato aquoso de alecrim. O ensaio in vivo foi conduzido em casa de vegetação climatizada, usando-se as mesmas concentrações do extrato de alecrim, pulverizado semanalmente sobre as plantas pelo período de 64 dias, iniciando no estádio fenológico V3. Três dias após a primeira pulverização inoculou-se 1800 ovos e 400 juvenis de segundo estágio (J2). O número de ovos e J2 no solo e raiz, o número de galhas e o fator de reprodução foram determinados. Os resultados indicaram que in vitro, o extrato de alecrim reduziu a eclosão de juvenis. Em soja, verificou-se redução de 48% no número de galhas, além da redução no número de ovos no solo e no fator de reprodução para a cultivar CD 206. Para CD 215 houve redução no número de ovos e no fator de reprodução. Assim, o extrato aquoso de alecrim apresenta potencial para manejo de M. incognita em soja.UEL2016-02-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2010610.5433/1679-0359.2016v37n1p103Semina: Ciências Agrárias; Vol. 37 No. 1 (2016); 103-110Semina: Ciências Agrárias; v. 37 n. 1 (2016); 103-1101679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20106/18056http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMüller, Mônica AnghinoniMioranza, Thaísa MurielStangarlin, José RenatoKuhn, Odair JoséBattistus, Andre GustavoIstchuk, Ademar NovaisFuchs, Felipe2022-12-02T14:15:10Zoai:ojs.pkp.sfu.ca:article/20106Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-02T14:15:10Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract Toxicidade in vitro e controle de Meloidogyne incognita em soja por extrato aquoso de alecrim |
title |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract |
spellingShingle |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract Müller, Mônica Anghinoni Alternative control Resistance induction Root-knot nematode Rosemary extract. Controle alternativo Indução de resistência Nematoide de galha Rosmarinus officinalis. |
title_short |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract |
title_full |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract |
title_fullStr |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract |
title_full_unstemmed |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract |
title_sort |
In vitro toxicity and control of Meloidogyne incognita in soybean by rosemary extract |
author |
Müller, Mônica Anghinoni |
author_facet |
Müller, Mônica Anghinoni Mioranza, Thaísa Muriel Stangarlin, José Renato Kuhn, Odair José Battistus, Andre Gustavo Istchuk, Ademar Novais Fuchs, Felipe |
author_role |
author |
author2 |
Mioranza, Thaísa Muriel Stangarlin, José Renato Kuhn, Odair José Battistus, Andre Gustavo Istchuk, Ademar Novais Fuchs, Felipe |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Müller, Mônica Anghinoni Mioranza, Thaísa Muriel Stangarlin, José Renato Kuhn, Odair José Battistus, Andre Gustavo Istchuk, Ademar Novais Fuchs, Felipe |
dc.subject.por.fl_str_mv |
Alternative control Resistance induction Root-knot nematode Rosemary extract. Controle alternativo Indução de resistência Nematoide de galha Rosmarinus officinalis. |
topic |
Alternative control Resistance induction Root-knot nematode Rosemary extract. Controle alternativo Indução de resistência Nematoide de galha Rosmarinus officinalis. |
description |
The control of nematodes in plants can be challenging, and there is a need for alternative, environmentally conscious methods for their management. The purpose of this study was to evaluate the effect of rosemary extract (Rosmarinus officinalis) on the in vitro toxicity and control of Meloidogyne incognita in CD 206 and CD 215 soybean cultivars. Using an in vitro assay, 500 M. incognita eggs per plate were observed for 15 days after incubation with rosemary extract at concentrations of 1%, 5%, and 10%. Soybean plants were studied under greenhouse conditions, and starting at V3 stage, were sprayed weekly with the same concentration of rosemary extract for 64 days. Three days after the first treatment, each soybean plant was inoculated with 1800 eggs and 400 second-stage juveniles (J2). At the end of this essay, number of eggs and J2 in the roots and soil, number of galls, and the reproduction factor (RF) were evaluated. Our results showed that in the in vitro assay, rosemary extract reduced the number of M. incognita eggs that hatched. Under greenhouse conditions, the CD 206 cultivar showed a 48% reduction in the number of galls, as well as fewer eggs in the soil and a lower RF. Similarly, in the CD 215 cultivar, the number of eggs was reduced and the RF was lower. These results indicate the potential for rosemary extract to control M. incognita in soybean crops. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20106 10.5433/1679-0359.2016v37n1p103 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20106 |
identifier_str_mv |
10.5433/1679-0359.2016v37n1p103 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/20106/18056 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 1 (2016); 103-110 Semina: Ciências Agrárias; v. 37 n. 1 (2016); 103-110 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1799306073246531584 |