Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936 |
Resumo: | Visceral Leishmaniasis (VL) is a parasitic zoonosis expanding in Brazil. Several municipalities in the state of Mato Grosso including those on the river Cuiabá have reported the incidence of both human and canine cases and the identification of sandfly vector, Lutzomyia longipalpis and Lu. cruzi. Dogs are considered the main reservoir of Leishmania chagasi in the urban areas, hence, we devised a cross-sectional study aimed at assessing the prevalence of the infection in the dogs of riverside communities on Cuiabá River watershed by parasitological (parasitic isolation in culture), serological, and molecular methods. Of the 248 surveyed dogs, 24 were positive in enzyme linked immunosorbent assay (ELISA) or immunofluorescence antibody test (IFAT), with a prevalence of 9.7%. The riverside communities located in the town of Santo Antonio do Leverger displayed a higher prevalence of the disease than the cities of Cuiabá and Várzea Grande; however, the difference was not statistically significant (p > 0.05). Dogs born in the communities had a 3.24-fold higher risk of acquiring the infection. Promastigote were isolated in the axenic culture from the bone marrow samples and intact skin. Further, DNA of Leishmania sp. was detected in the bone marrow samples, lymph nodes, leukocyte cover, and skin of only one examined dog. These samples were sequenced and they showed 99% homology to L. infantum. To conclude, we observed a higher prevalence of infection in Riverside communities of Santo Antonio do Leverger and the confirmation of autochthony in these areas justifies the surveillance actions to minimise the risk of transmission within the riverine community itself, besides its dissemination to other areas by tourism. |
id |
UEL-11_d3edd070166d5be73c9bad2e1a032110 |
---|---|
oai_identifier_str |
oai:ojs.pkp.sfu.ca:article/33936 |
network_acronym_str |
UEL-11 |
network_name_str |
Semina. Ciências Agrárias (Online) |
repository_id_str |
|
spelling |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershedLeishmaniose Visceral canina em comunidades ribeirinhas da bacia do rio CuiabáLeishmania infantumRiverside communitiesDogCuiaba.Leishmania infantumComunidades ribeirinhasCãoCuiabá.Visceral Leishmaniasis (VL) is a parasitic zoonosis expanding in Brazil. Several municipalities in the state of Mato Grosso including those on the river Cuiabá have reported the incidence of both human and canine cases and the identification of sandfly vector, Lutzomyia longipalpis and Lu. cruzi. Dogs are considered the main reservoir of Leishmania chagasi in the urban areas, hence, we devised a cross-sectional study aimed at assessing the prevalence of the infection in the dogs of riverside communities on Cuiabá River watershed by parasitological (parasitic isolation in culture), serological, and molecular methods. Of the 248 surveyed dogs, 24 were positive in enzyme linked immunosorbent assay (ELISA) or immunofluorescence antibody test (IFAT), with a prevalence of 9.7%. The riverside communities located in the town of Santo Antonio do Leverger displayed a higher prevalence of the disease than the cities of Cuiabá and Várzea Grande; however, the difference was not statistically significant (p > 0.05). Dogs born in the communities had a 3.24-fold higher risk of acquiring the infection. Promastigote were isolated in the axenic culture from the bone marrow samples and intact skin. Further, DNA of Leishmania sp. was detected in the bone marrow samples, lymph nodes, leukocyte cover, and skin of only one examined dog. These samples were sequenced and they showed 99% homology to L. infantum. To conclude, we observed a higher prevalence of infection in Riverside communities of Santo Antonio do Leverger and the confirmation of autochthony in these areas justifies the surveillance actions to minimise the risk of transmission within the riverine community itself, besides its dissemination to other areas by tourism.A Leishmaniose visceral é uma zoonose parasitária em expansão no Brasil. Em vários municípios do Estado de Mato Grosso têm sido notificados casos humanos e caninos, assim como a identificação de flebotomíneos vetores, Lutzomyia longipalpis e Lu. cruzi, inclusive em municípios às margens do Rio Cuiabá. Como o cão é considerado o principal reservatório da Leishmania infantum em áreas urbanas, delineou-se um estudo seccional com o objetivo de avaliar a prevalência da infecção em cães de comunidades ribeirinhas da Bacia do Rio Cuiabá por método parasitológico (isolamento parasitário em cultura), sorológico e molecular. Dos 248 cães pesquisados, 24 foram soro reagentes no ELISA ou IFI, com prevalência de 9,7%. As comunidades ribeirinhas localizadas na cidade de Santo Antônio do Leverger apresentaram maior prevalência que as dos municípios de Cuiabá e Várzea Grande, porém não estatisticamente significativo (p>0,05). Os cães nascidos nas comunidades apresentaram um risco 3,24 vezes maior de adquirir a infecção (p=0,03). Isolamento de promastigota em amostras de medula óssea e pele íntegra e DNA de Leishmania sp. foi detectado em amostras de medula óssea, linfonodo, capa leucocitária e pele íntegra de apenas um cão pesquisado. Tais amostras foram sequenciadas obtendo 99% de homologia a L. infantum. Em conclusão, se observou maior prevalência da infecção nas Comunidades Ribeirinhas do município de Santo Antônio do Leverger, e a comprovação de autoctonia nestas áreas justifica ações de vigilância que minimizem o risco de transmissão para a própria comunidade ribeirinha além da disseminação para outras áreas pelo turismo.UEL2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresepidemiological surveyapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3393610.5433/1679-0359.2019v40n6Supl2p3313Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3313-3320Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3313-33201679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936/26152Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSousa, Valéria Régia FrancoDias, Álvaro Felipe de Lima RuyRodrigues, Juliana YukiTorres, Mariana de MedeirosMoreira, Janaína Marcela Assunção RosaNakazato, LucianoDutra, ValériaAlmeida, Arleana do Bom Parto Ferreira de2022-10-10T16:34:28Zoai:ojs.pkp.sfu.ca:article/33936Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:34:28Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed Leishmaniose Visceral canina em comunidades ribeirinhas da bacia do rio Cuiabá |
title |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed |
spellingShingle |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed Sousa, Valéria Régia Franco Leishmania infantum Riverside communities Dog Cuiaba. Leishmania infantum Comunidades ribeirinhas Cão Cuiabá. |
title_short |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed |
title_full |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed |
title_fullStr |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed |
title_full_unstemmed |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed |
title_sort |
Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed |
author |
Sousa, Valéria Régia Franco |
author_facet |
Sousa, Valéria Régia Franco Dias, Álvaro Felipe de Lima Ruy Rodrigues, Juliana Yuki Torres, Mariana de Medeiros Moreira, Janaína Marcela Assunção Rosa Nakazato, Luciano Dutra, Valéria Almeida, Arleana do Bom Parto Ferreira de |
author_role |
author |
author2 |
Dias, Álvaro Felipe de Lima Ruy Rodrigues, Juliana Yuki Torres, Mariana de Medeiros Moreira, Janaína Marcela Assunção Rosa Nakazato, Luciano Dutra, Valéria Almeida, Arleana do Bom Parto Ferreira de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa, Valéria Régia Franco Dias, Álvaro Felipe de Lima Ruy Rodrigues, Juliana Yuki Torres, Mariana de Medeiros Moreira, Janaína Marcela Assunção Rosa Nakazato, Luciano Dutra, Valéria Almeida, Arleana do Bom Parto Ferreira de |
dc.subject.por.fl_str_mv |
Leishmania infantum Riverside communities Dog Cuiaba. Leishmania infantum Comunidades ribeirinhas Cão Cuiabá. |
topic |
Leishmania infantum Riverside communities Dog Cuiaba. Leishmania infantum Comunidades ribeirinhas Cão Cuiabá. |
description |
Visceral Leishmaniasis (VL) is a parasitic zoonosis expanding in Brazil. Several municipalities in the state of Mato Grosso including those on the river Cuiabá have reported the incidence of both human and canine cases and the identification of sandfly vector, Lutzomyia longipalpis and Lu. cruzi. Dogs are considered the main reservoir of Leishmania chagasi in the urban areas, hence, we devised a cross-sectional study aimed at assessing the prevalence of the infection in the dogs of riverside communities on Cuiabá River watershed by parasitological (parasitic isolation in culture), serological, and molecular methods. Of the 248 surveyed dogs, 24 were positive in enzyme linked immunosorbent assay (ELISA) or immunofluorescence antibody test (IFAT), with a prevalence of 9.7%. The riverside communities located in the town of Santo Antonio do Leverger displayed a higher prevalence of the disease than the cities of Cuiabá and Várzea Grande; however, the difference was not statistically significant (p > 0.05). Dogs born in the communities had a 3.24-fold higher risk of acquiring the infection. Promastigote were isolated in the axenic culture from the bone marrow samples and intact skin. Further, DNA of Leishmania sp. was detected in the bone marrow samples, lymph nodes, leukocyte cover, and skin of only one examined dog. These samples were sequenced and they showed 99% homology to L. infantum. To conclude, we observed a higher prevalence of infection in Riverside communities of Santo Antonio do Leverger and the confirmation of autochthony in these areas justifies the surveillance actions to minimise the risk of transmission within the riverine community itself, besides its dissemination to other areas by tourism. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-09-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Avaliado por pares epidemiological survey |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936 10.5433/1679-0359.2019v40n6Supl2p3313 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936 |
identifier_str_mv |
10.5433/1679-0359.2019v40n6Supl2p3313 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936/26152 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3313-3320 Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3313-3320 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306080339099648 |