Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed

Detalhes bibliográficos
Autor(a) principal: Sousa, Valéria Régia Franco
Data de Publicação: 2019
Outros Autores: Dias, Álvaro Felipe de Lima Ruy, Rodrigues, Juliana Yuki, Torres, Mariana de Medeiros, Moreira, Janaína Marcela Assunção Rosa, Nakazato, Luciano, Dutra, Valéria, Almeida, Arleana do Bom Parto Ferreira de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936
Resumo: Visceral Leishmaniasis (VL) is a parasitic zoonosis expanding in Brazil. Several municipalities in the state of Mato Grosso including those on the river Cuiabá have reported the incidence of both human and canine cases and the identification of sandfly vector, Lutzomyia longipalpis and Lu. cruzi. Dogs are considered the main reservoir of Leishmania chagasi in the urban areas, hence, we devised a cross-sectional study aimed at assessing the prevalence of the infection in the dogs of riverside communities on Cuiabá River watershed by parasitological (parasitic isolation in culture), serological, and molecular methods. Of the 248 surveyed dogs, 24 were positive in enzyme linked immunosorbent assay (ELISA) or immunofluorescence antibody test (IFAT), with a prevalence of 9.7%. The riverside communities located in the town of Santo Antonio do Leverger displayed a higher prevalence of the disease than the cities of Cuiabá and Várzea Grande; however, the difference was not statistically significant (p > 0.05). Dogs born in the communities had a 3.24-fold higher risk of acquiring the infection. Promastigote were isolated in the axenic culture from the bone marrow samples and intact skin. Further, DNA of Leishmania sp. was detected in the bone marrow samples, lymph nodes, leukocyte cover, and skin of only one examined dog. These samples were sequenced and they showed 99% homology to L. infantum. To conclude, we observed a higher prevalence of infection in Riverside communities of Santo Antonio do Leverger and the confirmation of autochthony in these areas justifies the surveillance actions to minimise the risk of transmission within the riverine community itself, besides its dissemination to other areas by tourism.
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spelling Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershedLeishmaniose Visceral canina em comunidades ribeirinhas da bacia do rio CuiabáLeishmania infantumRiverside communitiesDogCuiaba.Leishmania infantumComunidades ribeirinhasCãoCuiabá.Visceral Leishmaniasis (VL) is a parasitic zoonosis expanding in Brazil. Several municipalities in the state of Mato Grosso including those on the river Cuiabá have reported the incidence of both human and canine cases and the identification of sandfly vector, Lutzomyia longipalpis and Lu. cruzi. Dogs are considered the main reservoir of Leishmania chagasi in the urban areas, hence, we devised a cross-sectional study aimed at assessing the prevalence of the infection in the dogs of riverside communities on Cuiabá River watershed by parasitological (parasitic isolation in culture), serological, and molecular methods. Of the 248 surveyed dogs, 24 were positive in enzyme linked immunosorbent assay (ELISA) or immunofluorescence antibody test (IFAT), with a prevalence of 9.7%. The riverside communities located in the town of Santo Antonio do Leverger displayed a higher prevalence of the disease than the cities of Cuiabá and Várzea Grande; however, the difference was not statistically significant (p > 0.05). Dogs born in the communities had a 3.24-fold higher risk of acquiring the infection. Promastigote were isolated in the axenic culture from the bone marrow samples and intact skin. Further, DNA of Leishmania sp. was detected in the bone marrow samples, lymph nodes, leukocyte cover, and skin of only one examined dog. These samples were sequenced and they showed 99% homology to L. infantum. To conclude, we observed a higher prevalence of infection in Riverside communities of Santo Antonio do Leverger and the confirmation of autochthony in these areas justifies the surveillance actions to minimise the risk of transmission within the riverine community itself, besides its dissemination to other areas by tourism.A Leishmaniose visceral é uma zoonose parasitária em expansão no Brasil. Em vários municípios do Estado de Mato Grosso têm sido notificados casos humanos e caninos, assim como a identificação de flebotomíneos vetores, Lutzomyia longipalpis e Lu. cruzi, inclusive em municípios às margens do Rio Cuiabá. Como o cão é considerado o principal reservatório da Leishmania infantum em áreas urbanas, delineou-se um estudo seccional com o objetivo de avaliar a prevalência da infecção em cães de comunidades ribeirinhas da Bacia do Rio Cuiabá por método parasitológico (isolamento parasitário em cultura), sorológico e molecular. Dos 248 cães pesquisados, 24 foram soro reagentes no ELISA ou IFI, com prevalência de 9,7%. As comunidades ribeirinhas localizadas na cidade de Santo Antônio do Leverger apresentaram maior prevalência que as dos municípios de Cuiabá e Várzea Grande, porém não estatisticamente significativo (p>0,05). Os cães nascidos nas comunidades apresentaram um risco 3,24 vezes maior de adquirir a infecção (p=0,03). Isolamento de promastigota em amostras de medula óssea e pele íntegra e DNA de Leishmania sp. foi detectado em amostras de medula óssea, linfonodo, capa leucocitária e pele íntegra de apenas um cão pesquisado. Tais amostras foram sequenciadas obtendo 99% de homologia a L. infantum. Em conclusão, se observou maior prevalência da infecção nas Comunidades Ribeirinhas do município de Santo Antônio do Leverger, e a comprovação de autoctonia nestas áreas justifica ações de vigilância que minimizem o risco de transmissão para a própria comunidade ribeirinha além da disseminação para outras áreas pelo turismo.UEL2019-09-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionAvaliado por paresepidemiological surveyapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/3393610.5433/1679-0359.2019v40n6Supl2p3313Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3313-3320Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3313-33201679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936/26152Copyright (c) 2019 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessSousa, Valéria Régia FrancoDias, Álvaro Felipe de Lima RuyRodrigues, Juliana YukiTorres, Mariana de MedeirosMoreira, Janaína Marcela Assunção RosaNakazato, LucianoDutra, ValériaAlmeida, Arleana do Bom Parto Ferreira de2022-10-10T16:34:28Zoai:ojs.pkp.sfu.ca:article/33936Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-10-10T16:34:28Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
Leishmaniose Visceral canina em comunidades ribeirinhas da bacia do rio Cuiabá
title Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
spellingShingle Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
Sousa, Valéria Régia Franco
Leishmania infantum
Riverside communities
Dog
Cuiaba.
Leishmania infantum
Comunidades ribeirinhas
Cão
Cuiabá.
title_short Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
title_full Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
title_fullStr Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
title_full_unstemmed Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
title_sort Canine visceral leishmaniasis in riverside communities of the Cuiabá river watershed
author Sousa, Valéria Régia Franco
author_facet Sousa, Valéria Régia Franco
Dias, Álvaro Felipe de Lima Ruy
Rodrigues, Juliana Yuki
Torres, Mariana de Medeiros
Moreira, Janaína Marcela Assunção Rosa
Nakazato, Luciano
Dutra, Valéria
Almeida, Arleana do Bom Parto Ferreira de
author_role author
author2 Dias, Álvaro Felipe de Lima Ruy
Rodrigues, Juliana Yuki
Torres, Mariana de Medeiros
Moreira, Janaína Marcela Assunção Rosa
Nakazato, Luciano
Dutra, Valéria
Almeida, Arleana do Bom Parto Ferreira de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sousa, Valéria Régia Franco
Dias, Álvaro Felipe de Lima Ruy
Rodrigues, Juliana Yuki
Torres, Mariana de Medeiros
Moreira, Janaína Marcela Assunção Rosa
Nakazato, Luciano
Dutra, Valéria
Almeida, Arleana do Bom Parto Ferreira de
dc.subject.por.fl_str_mv Leishmania infantum
Riverside communities
Dog
Cuiaba.
Leishmania infantum
Comunidades ribeirinhas
Cão
Cuiabá.
topic Leishmania infantum
Riverside communities
Dog
Cuiaba.
Leishmania infantum
Comunidades ribeirinhas
Cão
Cuiabá.
description Visceral Leishmaniasis (VL) is a parasitic zoonosis expanding in Brazil. Several municipalities in the state of Mato Grosso including those on the river Cuiabá have reported the incidence of both human and canine cases and the identification of sandfly vector, Lutzomyia longipalpis and Lu. cruzi. Dogs are considered the main reservoir of Leishmania chagasi in the urban areas, hence, we devised a cross-sectional study aimed at assessing the prevalence of the infection in the dogs of riverside communities on Cuiabá River watershed by parasitological (parasitic isolation in culture), serological, and molecular methods. Of the 248 surveyed dogs, 24 were positive in enzyme linked immunosorbent assay (ELISA) or immunofluorescence antibody test (IFAT), with a prevalence of 9.7%. The riverside communities located in the town of Santo Antonio do Leverger displayed a higher prevalence of the disease than the cities of Cuiabá and Várzea Grande; however, the difference was not statistically significant (p > 0.05). Dogs born in the communities had a 3.24-fold higher risk of acquiring the infection. Promastigote were isolated in the axenic culture from the bone marrow samples and intact skin. Further, DNA of Leishmania sp. was detected in the bone marrow samples, lymph nodes, leukocyte cover, and skin of only one examined dog. These samples were sequenced and they showed 99% homology to L. infantum. To conclude, we observed a higher prevalence of infection in Riverside communities of Santo Antonio do Leverger and the confirmation of autochthony in these areas justifies the surveillance actions to minimise the risk of transmission within the riverine community itself, besides its dissemination to other areas by tourism.
publishDate 2019
dc.date.none.fl_str_mv 2019-09-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Avaliado por pares
epidemiological survey
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936
10.5433/1679-0359.2019v40n6Supl2p3313
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936
identifier_str_mv 10.5433/1679-0359.2019v40n6Supl2p3313
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/33936/26152
dc.rights.driver.fl_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
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publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 40 No. 6Supl2 (2019); 3313-3320
Semina: Ciências Agrárias; v. 40 n. 6Supl2 (2019); 3313-3320
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
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instname_str Universidade Estadual de Londrina (UEL)
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reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
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repository.mail.fl_str_mv semina.agrarias@uel.br
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