Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006

Detalhes bibliográficos
Autor(a) principal: Guide, Bruna Aparecida
Data de Publicação: 2023
Outros Autores: Alves, Viviane Sandra, França, Emanuele Julio Galvão de, Fernandes, Thiago Augusto Paes, Andrade, Nathália Costalonga, Oliveira Janeiro Neves, Pedro Manuel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/47664
Resumo: The objective of this study was to describe phenotypically and biochemically the symbiotic bacteria associated with three populations of Heterorhabditis amazonensis Andalo et al., 2006 (isolates: UEL-n 01, UEL-n 07, and UEL-n 08) and evaluate their pathogenicity on Galleria mellonella L. (Lepidoptera: Pyralidae) larvae. Bacteria were isolated by maceration of infective juveniles (IJs) and grown in culture medium (NBTA and MacConkey). The characterization of the bacteria was evaluated by employing motility test and biochemical tests like Gram staining, lipase activity, protease, and lecithinase. The production of antibiotics and bioluminescence was also evaluated. The pathogenicity was evaluated on the last instar larvae of G. mellonella at a concentration of 104 cells/mL. The bacteria from the three entomopathogenic nematodes isolates were positive for all biochemical tests except for lecithinase, and have presented bioluminescence when subjected to ultraviolet light, indicating that they belong to the genus Photorhabdus sp. Both were pathogenic to G. mellonella larvae causing 93.3 to 100.0% mortality.
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spelling Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006Caracterização fenotípica, bioquímica e avaliação da patogenicidade à Galleria mellonella L. (Lepidoptera: Pyralidae) de bactérias simbiontes do nematoide entomopatogênico Heterorhabditis amazonensis Andalo et al., 2006Microbial controlPhotorhabdusSymbiotic associationVirulence. Associação simbióticaControle microbianoControle microbianoPhotorhabdusVirulência.The objective of this study was to describe phenotypically and biochemically the symbiotic bacteria associated with three populations of Heterorhabditis amazonensis Andalo et al., 2006 (isolates: UEL-n 01, UEL-n 07, and UEL-n 08) and evaluate their pathogenicity on Galleria mellonella L. (Lepidoptera: Pyralidae) larvae. Bacteria were isolated by maceration of infective juveniles (IJs) and grown in culture medium (NBTA and MacConkey). The characterization of the bacteria was evaluated by employing motility test and biochemical tests like Gram staining, lipase activity, protease, and lecithinase. The production of antibiotics and bioluminescence was also evaluated. The pathogenicity was evaluated on the last instar larvae of G. mellonella at a concentration of 104 cells/mL. The bacteria from the three entomopathogenic nematodes isolates were positive for all biochemical tests except for lecithinase, and have presented bioluminescence when subjected to ultraviolet light, indicating that they belong to the genus Photorhabdus sp. Both were pathogenic to G. mellonella larvae causing 93.3 to 100.0% mortality.O objetivo desse trabalho foi descrever fenotípica e bioquimicamente as bactérias simbiontes associadas a três isolados do nematoide Heterorhabditis amazonensis Andalo et al., 2006 (isolates: UEL-n 01, UEL-n 07, and UEL-n 08) e avaliar sua patogenicidade sobre lagartas de Galleria mellonella L. (Lepidoptera: Pyralidae). As bactérias foram isoladas por meio de macerado de juvenis infectantes (JIs) e cultivadas em meio de cultura (NBTA e MacConkey). A caracterização das bactérias foi realizada por meio de testes de motilidade e bioquímicos de coloração de Gram, atividade de lipase, protease e lecitinase. Avaliou-se também a produção de antibióticos e de bioluminescência. A patogenicidade foi avaliada em lagartas de G. mellonella na concentração de 104 células/mL. Observou-se que as bactérias dos três isolados de nematoides entomopatogênicos foram positivas para todos os testes bioquímicos, com exceção da lecitinase, e apresentaram bioluminescência quando submetidas a luz ultravioleta, indicando que estas cepas pertencem ao gênero Photorhabdus sp. Ambas foram patogênicas às lagartas de G. mellonella causando mortalidade entre 93,3 e 100,0%.UEL2023-08-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/4766410.5433/1679-0359.2023v44n3p1047Semina: Ciências Agrárias; Vol. 44 No. 3 (2023); 1047-1058Semina: Ciências Agrárias; v. 44 n. 3 (2023); 1047-10581679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/47664/49254Copyright (c) 2023 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessGuide, Bruna AparecidaAlves, Viviane SandraFrança, Emanuele Julio Galvão deFernandes, Thiago Augusto PaesAndrade, Nathália CostalongaOliveira Janeiro Neves, Pedro Manuel2023-10-03T12:29:43Zoai:ojs.pkp.sfu.ca:article/47664Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-10-03T12:29:43Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
Caracterização fenotípica, bioquímica e avaliação da patogenicidade à Galleria mellonella L. (Lepidoptera: Pyralidae) de bactérias simbiontes do nematoide entomopatogênico Heterorhabditis amazonensis Andalo et al., 2006
title Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
spellingShingle Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
Guide, Bruna Aparecida
Microbial control
Photorhabdus
Symbiotic association
Virulence.
Associação simbiótica
Controle microbianoControle microbiano
Photorhabdus
Virulência.
title_short Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
title_full Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
title_fullStr Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
title_full_unstemmed Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
title_sort Phenotypic and biochemical characterisation and pathogenicity assessment on Galleria mellonella L. (Lepidoptera: Pyralidae) of symbionts of the entomopathogenic nematode Heterorhabditis amazonensis Andalo et al., 2006
author Guide, Bruna Aparecida
author_facet Guide, Bruna Aparecida
Alves, Viviane Sandra
França, Emanuele Julio Galvão de
Fernandes, Thiago Augusto Paes
Andrade, Nathália Costalonga
Oliveira Janeiro Neves, Pedro Manuel
author_role author
author2 Alves, Viviane Sandra
França, Emanuele Julio Galvão de
Fernandes, Thiago Augusto Paes
Andrade, Nathália Costalonga
Oliveira Janeiro Neves, Pedro Manuel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Guide, Bruna Aparecida
Alves, Viviane Sandra
França, Emanuele Julio Galvão de
Fernandes, Thiago Augusto Paes
Andrade, Nathália Costalonga
Oliveira Janeiro Neves, Pedro Manuel
dc.subject.por.fl_str_mv Microbial control
Photorhabdus
Symbiotic association
Virulence.
Associação simbiótica
Controle microbianoControle microbiano
Photorhabdus
Virulência.
topic Microbial control
Photorhabdus
Symbiotic association
Virulence.
Associação simbiótica
Controle microbianoControle microbiano
Photorhabdus
Virulência.
description The objective of this study was to describe phenotypically and biochemically the symbiotic bacteria associated with three populations of Heterorhabditis amazonensis Andalo et al., 2006 (isolates: UEL-n 01, UEL-n 07, and UEL-n 08) and evaluate their pathogenicity on Galleria mellonella L. (Lepidoptera: Pyralidae) larvae. Bacteria were isolated by maceration of infective juveniles (IJs) and grown in culture medium (NBTA and MacConkey). The characterization of the bacteria was evaluated by employing motility test and biochemical tests like Gram staining, lipase activity, protease, and lecithinase. The production of antibiotics and bioluminescence was also evaluated. The pathogenicity was evaluated on the last instar larvae of G. mellonella at a concentration of 104 cells/mL. The bacteria from the three entomopathogenic nematodes isolates were positive for all biochemical tests except for lecithinase, and have presented bioluminescence when subjected to ultraviolet light, indicating that they belong to the genus Photorhabdus sp. Both were pathogenic to G. mellonella larvae causing 93.3 to 100.0% mortality.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-14
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/47664
10.5433/1679-0359.2023v44n3p1047
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/47664
identifier_str_mv 10.5433/1679-0359.2023v44n3p1047
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/47664/49254
dc.rights.driver.fl_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2023 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 44 No. 3 (2023); 1047-1058
Semina: Ciências Agrárias; v. 44 n. 3 (2023); 1047-1058
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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