Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009 |
Resumo: | The anthelmintic effect of Morinda citrifolia (noni) on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1). The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV) during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water) (p>0.05) in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p<0.05). In the concentration of 26.96 mg.mL-1, for the in vitro test, the aqueous extract presented a mortality of 100%, the same obtained by piperazin (100%), differing statistically from the negative control (p<0.05). In the concentrations of 33.36 and 66.72 mg.mL-1, the ethanolic extract also presented a mortality of 100%, there was a significant statistical difference from the negative control (p<0,05). Noni fruit extracts manifested high anthelmintic activity in vitro, however, more studies are necessary with higher concentrations in the in vivo test. |
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Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarumAção anti-helmíntica da Morinda citrifolia (noni) sobre Heterakis gallinarumMedicinal plantsHelminthologyChicken.Plantas medicinaisHelmintologiaGalinha.The anthelmintic effect of Morinda citrifolia (noni) on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1). The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV) during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water) (p>0.05) in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p<0.05). In the concentration of 26.96 mg.mL-1, for the in vitro test, the aqueous extract presented a mortality of 100%, the same obtained by piperazin (100%), differing statistically from the negative control (p<0.05). In the concentrations of 33.36 and 66.72 mg.mL-1, the ethanolic extract also presented a mortality of 100%, there was a significant statistical difference from the negative control (p<0,05). Noni fruit extracts manifested high anthelmintic activity in vitro, however, more studies are necessary with higher concentrations in the in vivo test.A ação anti-helmíntica da Morinda citrifolia (noni) sobre Heterakis gallinarum foi avaliada em galinhas poedeiras naturalmente infectadas. A atividade anti-helmíntica in vitro foi determinada em helmintos adultos colocados em placas de petri descartáveis, contendo solução Tyrode pré-aquecida, nas quais foi adicionado o extrato aquoso ou etanólico e mantidas em BOD a uma temperatura de 37oC (±1). Os extratos aquoso e etanólico foram usados nas seguintes concentrações: 1,69; 3,37; 6,74; 13,48 e 26,96 mg.mL-1 e 4,17; 8,34; 16,68; 33,36 e 66,72 mg.mL-1, respectivamente. Como controle positivo usou-se uma solução de citrato de piperazina tetrahidratada na concentração de 50 mg.mL-1. A atividade anti-helmíntica in vivo foi determinada em aves, administrando-se os extratos aquoso ou etanólico (10 mL/Kg/PV) durante três dias consecutivos. As fezes dos animais foram coletadas durante quatro dias, em seguida foram lavadas em água corrente e peneiradas. No quinto dia após início do tratamento, as aves foram abatidas e necropsiadas, para contagem e identificação dos helmintos remanescentes. Os dados obtidos foram analisados estatisticamente utilizando o teste de Student-Newman-Keuls. No teste in vivo, não houve diferença significativa entre o extrato aquoso (concentração de 10%) e o grupo controle (água) (p>0,05) na eliminação de H. gallinarum. O extrato etanólico apresentou um percentual de eliminação de 20,35%, diferindo estatisticamente do grupo controle (p<0,05). Na concentração 26,96 mg.mL-1, para o teste in vitro, o extrato aquoso causou mortalidade de 100%, semelhante ao obtido pela piperazina (100%), diferindo estatisticamente do controle negativo (p<0,05). Nas concentrações 33,36 e 66,72 mg.mL-1, o extrato etanólico também apresentou percentagem de mortalidade de 100%, havendo diferença estatisticamente significativa do controle negativo (P<0,05). Conclui-se que a atividade anti-helmíntica do fruto do noni apresentou resultados promissores no teste in vitro, havendo necessidade de estudos com maiores concentrações no teste in vivo.UEL2013-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Experimentalapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1200910.5433/1679-0359.2013v34n4p1775Semina: Ciências Agrárias; Vol. 34 No. 4 (2013); 1775-1782Semina: Ciências Agrárias; v. 34 n. 4 (2013); 1775-17821679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009/pdfCopyright (c) 2013 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBrito, Danilo Rodrigues BarrosFernandes, Rozeverter Moreno2023-01-16T16:42:53Zoai:ojs.pkp.sfu.ca:article/12009Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-16T16:42:53Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum Ação anti-helmíntica da Morinda citrifolia (noni) sobre Heterakis gallinarum |
title |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum |
spellingShingle |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum Brito, Danilo Rodrigues Barros Medicinal plants Helminthology Chicken. Plantas medicinais Helmintologia Galinha. |
title_short |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum |
title_full |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum |
title_fullStr |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum |
title_full_unstemmed |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum |
title_sort |
Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum |
author |
Brito, Danilo Rodrigues Barros |
author_facet |
Brito, Danilo Rodrigues Barros Fernandes, Rozeverter Moreno |
author_role |
author |
author2 |
Fernandes, Rozeverter Moreno |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Brito, Danilo Rodrigues Barros Fernandes, Rozeverter Moreno |
dc.subject.por.fl_str_mv |
Medicinal plants Helminthology Chicken. Plantas medicinais Helmintologia Galinha. |
topic |
Medicinal plants Helminthology Chicken. Plantas medicinais Helmintologia Galinha. |
description |
The anthelmintic effect of Morinda citrifolia (noni) on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1). The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV) during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water) (p>0.05) in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p<0.05). In the concentration of 26.96 mg.mL-1, for the in vitro test, the aqueous extract presented a mortality of 100%, the same obtained by piperazin (100%), differing statistically from the negative control (p<0.05). In the concentrations of 33.36 and 66.72 mg.mL-1, the ethanolic extract also presented a mortality of 100%, there was a significant statistical difference from the negative control (p<0,05). Noni fruit extracts manifested high anthelmintic activity in vitro, however, more studies are necessary with higher concentrations in the in vivo test. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Experimental |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009 10.5433/1679-0359.2013v34n4p1775 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009 |
identifier_str_mv |
10.5433/1679-0359.2013v34n4p1775 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2013 Semina: Ciências Agrárias http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 34 No. 4 (2013); 1775-1782 Semina: Ciências Agrárias; v. 34 n. 4 (2013); 1775-1782 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306067162693632 |