Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum

Detalhes bibliográficos
Autor(a) principal: Brito, Danilo Rodrigues Barros
Data de Publicação: 2013
Outros Autores: Fernandes, Rozeverter Moreno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009
Resumo: The anthelmintic effect of Morinda citrifolia (noni) on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1). The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV) during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water) (p>0.05) in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p<0.05). In the concentration of 26.96 mg.mL-1, for the in vitro test, the aqueous extract presented a mortality of 100%, the same obtained by piperazin (100%), differing statistically from the negative control (p<0.05). In the concentrations of 33.36 and 66.72 mg.mL-1, the ethanolic extract also presented a mortality of 100%, there was a significant statistical difference from the negative control (p<0,05). Noni fruit extracts manifested high anthelmintic activity in vitro, however, more studies are necessary with higher concentrations in the in vivo test.
id UEL-11_ddc0554429b82a5973831347fe4f41c4
oai_identifier_str oai:ojs.pkp.sfu.ca:article/12009
network_acronym_str UEL-11
network_name_str Semina. Ciências Agrárias (Online)
repository_id_str
spelling Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarumAção anti-helmíntica da Morinda citrifolia (noni) sobre Heterakis gallinarumMedicinal plantsHelminthologyChicken.Plantas medicinaisHelmintologiaGalinha.The anthelmintic effect of Morinda citrifolia (noni) on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1). The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV) during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water) (p>0.05) in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p<0.05). In the concentration of 26.96 mg.mL-1, for the in vitro test, the aqueous extract presented a mortality of 100%, the same obtained by piperazin (100%), differing statistically from the negative control (p<0.05). In the concentrations of 33.36 and 66.72 mg.mL-1, the ethanolic extract also presented a mortality of 100%, there was a significant statistical difference from the negative control (p<0,05). Noni fruit extracts manifested high anthelmintic activity in vitro, however, more studies are necessary with higher concentrations in the in vivo test.A ação anti-helmíntica da Morinda citrifolia (noni) sobre Heterakis gallinarum foi avaliada em galinhas poedeiras naturalmente infectadas. A atividade anti-helmíntica in vitro foi determinada em helmintos adultos colocados em placas de petri descartáveis, contendo solução Tyrode pré-aquecida, nas quais foi adicionado o extrato aquoso ou etanólico e mantidas em BOD a uma temperatura de 37oC (±1). Os extratos aquoso e etanólico foram usados nas seguintes concentrações: 1,69; 3,37; 6,74; 13,48 e 26,96 mg.mL-1 e 4,17; 8,34; 16,68; 33,36 e 66,72 mg.mL-1, respectivamente. Como controle positivo usou-se uma solução de citrato de piperazina tetrahidratada na concentração de 50 mg.mL-1. A atividade anti-helmíntica in vivo foi determinada em aves, administrando-se os extratos aquoso ou etanólico (10 mL/Kg/PV) durante três dias consecutivos. As fezes dos animais foram coletadas durante quatro dias, em seguida foram lavadas em água corrente e peneiradas. No quinto dia após início do tratamento, as aves foram abatidas e necropsiadas, para contagem e identificação dos helmintos remanescentes. Os dados obtidos foram analisados estatisticamente utilizando o teste de Student-Newman-Keuls. No teste in vivo, não houve diferença significativa entre o extrato aquoso (concentração de 10%) e o grupo controle (água) (p>0,05) na eliminação de H. gallinarum. O extrato etanólico apresentou um percentual de eliminação de 20,35%, diferindo estatisticamente do grupo controle (p<0,05). Na concentração 26,96 mg.mL-1, para o teste in vitro, o extrato aquoso causou mortalidade de 100%, semelhante ao obtido pela piperazina (100%), diferindo estatisticamente do controle negativo (p<0,05). Nas concentrações 33,36 e 66,72 mg.mL-1, o extrato etanólico também apresentou percentagem de mortalidade de 100%, havendo diferença estatisticamente significativa do controle negativo (P<0,05). Conclui-se que a atividade anti-helmíntica do fruto do noni apresentou resultados promissores no teste in vitro, havendo necessidade de estudos com maiores concentrações no teste in vivo.UEL2013-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Experimentalapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1200910.5433/1679-0359.2013v34n4p1775Semina: Ciências Agrárias; Vol. 34 No. 4 (2013); 1775-1782Semina: Ciências Agrárias; v. 34 n. 4 (2013); 1775-17821679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009/pdfCopyright (c) 2013 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessBrito, Danilo Rodrigues BarrosFernandes, Rozeverter Moreno2023-01-16T16:42:53Zoai:ojs.pkp.sfu.ca:article/12009Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-16T16:42:53Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
Ação anti-helmíntica da Morinda citrifolia (noni) sobre Heterakis gallinarum
title Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
spellingShingle Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
Brito, Danilo Rodrigues Barros
Medicinal plants
Helminthology
Chicken.
Plantas medicinais
Helmintologia
Galinha.
title_short Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
title_full Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
title_fullStr Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
title_full_unstemmed Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
title_sort Anthelmintic action of Morinda citrifolia (noni) on Heterakis gallinarum
author Brito, Danilo Rodrigues Barros
author_facet Brito, Danilo Rodrigues Barros
Fernandes, Rozeverter Moreno
author_role author
author2 Fernandes, Rozeverter Moreno
author2_role author
dc.contributor.author.fl_str_mv Brito, Danilo Rodrigues Barros
Fernandes, Rozeverter Moreno
dc.subject.por.fl_str_mv Medicinal plants
Helminthology
Chicken.
Plantas medicinais
Helmintologia
Galinha.
topic Medicinal plants
Helminthology
Chicken.
Plantas medicinais
Helmintologia
Galinha.
description The anthelmintic effect of Morinda citrifolia (noni) on Heterakis gallinarum was evaluated in chicken naturally infected. The anthelmintic activity in vitro was determined in adults helminthes in disposable petri dishes, containing Tyrode solution, pre warmed in which aqueous or ethanolic extracts were added. The material was maintained in a BOD at 37ºC (±1). The aqueous and ethanolic extracts presented the following concentrations: 1.69; 3.37; 6.74; 13.48 e 26.96 mg.mL-1 and 4.17; 8.34; 16.68; 33.36 e 66.72 mg.mL-1, respectively. It was used as positive control, a solution of tetrahidrate citrate of piperazin in the concentration of 50 mg/mL. The anthelmintic activity in vivo was determined by the administration of aqueous or ethanolic extracts (10 mL/Kg/PV) during three consecutive days. The feces were collected during four days in each group, washed in water and sifted. In the fifth day post-treatment, the chickens were slaughtered and necropsy was performed in order to count and identify remaining helminthes. The data were analyzed by the Student-Newman-Keuls test. In the in vivo test there was no significant difference between the aqueous extract and the control group (water) (p>0.05) in the elimination of H. gallinarum. The ethanolic extract presented an elimination of 20.35%, differing statistically from the control group (p<0.05). In the concentration of 26.96 mg.mL-1, for the in vitro test, the aqueous extract presented a mortality of 100%, the same obtained by piperazin (100%), differing statistically from the negative control (p<0.05). In the concentrations of 33.36 and 66.72 mg.mL-1, the ethanolic extract also presented a mortality of 100%, there was a significant statistical difference from the negative control (p<0,05). Noni fruit extracts manifested high anthelmintic activity in vitro, however, more studies are necessary with higher concentrations in the in vivo test.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Experimental
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009
10.5433/1679-0359.2013v34n4p1775
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009
identifier_str_mv 10.5433/1679-0359.2013v34n4p1775
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12009/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2013 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2013 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 34 No. 4 (2013); 1775-1782
Semina: Ciências Agrárias; v. 34 n. 4 (2013); 1775-1782
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
_version_ 1799306067162693632