Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16265 |
Resumo: | Canine leproid granuloma or “canine leprosy” is a mycobacteriosis that has a dermatopathic framework, which includes aspects that are still unclear, especially regarding its mode of transmission and etiological agent. In order to verify the genetic characterization of the mycobacteria that is involved in the condition, the present study analyzed histological cuts from animals with an established diagnosis of such disorder. The study samples were taken from of 13 dogs that were assisted at the HOVET / USP Dermatology Service. This material was subsequently subjected to “nested” and real-time polymerase chain reaction (PCR). The nested PCR confirmed the presence of the agent in 18.7% of the animals, whereas the real-time PCR detected 69.2% of samples with DNA of the mycobacterium, thus proving that such method presents higher sensitivity. Furthermore, it was observed that the species of mycobacterium that causes Canine Leproid Granulnoma in dogs from Brazil presents a 100% genetic homology when compared to samples obtained from animals in studies abroad. |
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Semina. Ciências Agrárias (Online) |
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Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009)Diagnóstico molecular do granuloma lepróide canino, a partir de cortes histológicos emblocados em parafina, pela técnica de reação em cadeia de polimerase (Pcr) – estudo retrospectivo (2002-2009)Leproid GranulomaDogsPCR.Granuloma lepróideCãesPCR.Canine leproid granuloma or “canine leprosy” is a mycobacteriosis that has a dermatopathic framework, which includes aspects that are still unclear, especially regarding its mode of transmission and etiological agent. In order to verify the genetic characterization of the mycobacteria that is involved in the condition, the present study analyzed histological cuts from animals with an established diagnosis of such disorder. The study samples were taken from of 13 dogs that were assisted at the HOVET / USP Dermatology Service. This material was subsequently subjected to “nested” and real-time polymerase chain reaction (PCR). The nested PCR confirmed the presence of the agent in 18.7% of the animals, whereas the real-time PCR detected 69.2% of samples with DNA of the mycobacterium, thus proving that such method presents higher sensitivity. Furthermore, it was observed that the species of mycobacterium that causes Canine Leproid Granulnoma in dogs from Brazil presents a 100% genetic homology when compared to samples obtained from animals in studies abroad.O Granuloma lepróide canino (GLC) ou “lepra canina” consiste em micobacteriose, com quadro dermatopático que conta com aspectos ainda obscuros, principalmente quanto ao seu modo de transmissão e agente etiológico envolvido. Com a finalidade de se verificar a caracterização gênica da micobactéria em questão, procedeu-se estudo a partir de cortes histológicos, de animais com diagnóstico estabelecido da enfermidade. A amostragem foi composta de 13 cães, atendidos no Serviço de Dermatologia do HOVET/USP. Tal material foi posteriormente submetido às técnicas de reação em cadeia de polimerase (PCR), “nested” e em tempo real. O método da PCR “nested” confirmou a presença do agente em apenas 18,7% dos animais. Enquanto que, em 69,2% das amostras, o DNA da micobactéria envolvida foi detectado pela PCR em tempo real, demonstrando ser neste estudo, o método de maior sensibilidade. Pôde-se concluir ainda que a espécie de micobactéria causadora do Granuloma lepróide canino nos cães brasileiros possui homologia gênica de 100%, quando comparada às amostras provindas de animais de estudos estrangeiros. UEL2015-10-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1626510.5433/1679-0359.2015v36n5p3129Semina: Ciências Agrárias; Vol. 36 No. 5 (2015); 3129-3138Semina: Ciências Agrárias; v. 36 n. 5 (2015); 3129-31381679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16265/17263http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMaruyama, SimoniBrandão, Paulo EduardoCastro, Alessandra Marnie Martins Gomes deMichalany, Nilceo SchweryFyfe, JanetMalik, RichardLarsson, Carlos Eduardo2023-01-12T13:12:31Zoai:ojs.pkp.sfu.ca:article/16265Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-12T13:12:31Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) Diagnóstico molecular do granuloma lepróide canino, a partir de cortes histológicos emblocados em parafina, pela técnica de reação em cadeia de polimerase (Pcr) – estudo retrospectivo (2002-2009) |
title |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) |
spellingShingle |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) Maruyama, Simoni Leproid Granuloma Dogs PCR. Granuloma lepróide Cães PCR. |
title_short |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) |
title_full |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) |
title_fullStr |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) |
title_full_unstemmed |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) |
title_sort |
Molecular diagnosis of Canine Leproid Granuloma out of paraffin-embedded histological cuts using polymerase chain reaction (PCR) – a retrospective study (2002-2009) |
author |
Maruyama, Simoni |
author_facet |
Maruyama, Simoni Brandão, Paulo Eduardo Castro, Alessandra Marnie Martins Gomes de Michalany, Nilceo Schwery Fyfe, Janet Malik, Richard Larsson, Carlos Eduardo |
author_role |
author |
author2 |
Brandão, Paulo Eduardo Castro, Alessandra Marnie Martins Gomes de Michalany, Nilceo Schwery Fyfe, Janet Malik, Richard Larsson, Carlos Eduardo |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Maruyama, Simoni Brandão, Paulo Eduardo Castro, Alessandra Marnie Martins Gomes de Michalany, Nilceo Schwery Fyfe, Janet Malik, Richard Larsson, Carlos Eduardo |
dc.subject.por.fl_str_mv |
Leproid Granuloma Dogs PCR. Granuloma lepróide Cães PCR. |
topic |
Leproid Granuloma Dogs PCR. Granuloma lepróide Cães PCR. |
description |
Canine leproid granuloma or “canine leprosy” is a mycobacteriosis that has a dermatopathic framework, which includes aspects that are still unclear, especially regarding its mode of transmission and etiological agent. In order to verify the genetic characterization of the mycobacteria that is involved in the condition, the present study analyzed histological cuts from animals with an established diagnosis of such disorder. The study samples were taken from of 13 dogs that were assisted at the HOVET / USP Dermatology Service. This material was subsequently subjected to “nested” and real-time polymerase chain reaction (PCR). The nested PCR confirmed the presence of the agent in 18.7% of the animals, whereas the real-time PCR detected 69.2% of samples with DNA of the mycobacterium, thus proving that such method presents higher sensitivity. Furthermore, it was observed that the species of mycobacterium that causes Canine Leproid Granulnoma in dogs from Brazil presents a 100% genetic homology when compared to samples obtained from animals in studies abroad. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16265 10.5433/1679-0359.2015v36n5p3129 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16265 |
identifier_str_mv |
10.5433/1679-0359.2015v36n5p3129 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/16265/17263 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 36 No. 5 (2015); 3129-3138 Semina: Ciências Agrárias; v. 36 n. 5 (2015); 3129-3138 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306070827466752 |