Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Semina. Ciências Agrárias (Online) |
DOI: | 10.5433/1679-0359.2016v37n4p2167 |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21997 |
Resumo: | Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide – DMSO, Glucose 5% + 10% Methyl glycol – MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen. |
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Semina. Ciências Agrárias (Online) |
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Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machineCriopreservação do sêmen de tambaqui utilizando dry shipper e máquina de congelação programadaColossoma macropomumConservation methodsExtendersSperm class analyzerTeleost.Colossoma macropomumDiluidoresMétodos de conservaçãoSperm class analyzer.Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide – DMSO, Glucose 5% + 10% Methyl glycol – MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen.O tambaqui (Colossoma macropomum) é uma espécie nativa de peixe de água doce de grande importância para aquicultura brasileira. Devido a isso, diversas técnicas têm sido desenvolvidas para aperfeiçoar a reprodução desta espécie em cativeiro, dentre elas a criopreservação de sêmen de peixe. Como uma forma de melhorar os protocolos de criopreservação, tem-se buscado utilizar soluções diluidoras e métodos de congelação adequados, proporcionando uma boa qualidade seminal pós-descongelação. Dessa forma, este estudo objetivou avaliar a eficiência de diferentes diluidores e métodos de congelação na criopreservação do sêmen de tambaqui (C. macropomum). As amostras de sêmen fresco foram diluídas em diferentes tratamentos (Glicose 5% + 10% Dimetilsufóxido – DMSO; Glicose 5% + 10% Metil glicol – MG; Beltsville Thawing Solution – BTS + 10% DMSO e BTS + 10% MG) na proporção 1:9 e congeladas em máquina de congelação programada e em Dry shipper. As amostras seminais foram descongeladas e avaliadas para vitalidade, morfologia e cinética espermáticas. O sêmen criopreservado com DMSO utilizando a máquina de congelação programada proporcionou maiores percentuais de espermatozoides móveis (15,44 ± 1,04%) após a descongelação em relação ao Dry shipper (3,99 ± 0,55%), independente do diluente utilizado. Além disso, DMSO proporcionou as melhores velocidades espermáticas em relação ao MG, independente do método de congelação e diluente empregado. Um maior percentual de espermatozoides vivos foi observado quando se utilizou Glicose (37,28 ± 1,32%) como diluente (independente do método e crioprotetor), e DMSO (37,98 ± 1,25%) em máquina de congelação programada. Para a morfologia espermática, uma maior quantidade de espermatozoides normais (46,10 ± 1,82%) foi observada quando o sêmen foi criopreservado usando a máquina de congelação programada com o DMSO, para os crioprotetores, Glicose e BTS (38,16 ± 1,9%; 39,26 ± 1,87%, respectivamente), para os diluentes. Portanto, sugere-se a utilização do crioprotetor DMSO 10% associado ao diluente Glicose 5% em Máquina de Congelação Programada, para a criopreservação do sêmen de C. macropomum.UEL2016-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2199710.5433/1679-0359.2016v37n4p2167Semina: Ciências Agrárias; Vol. 37 No. 4 (2016); 2167-2180Semina: Ciências Agrárias; v. 37 n. 4 (2016); 2167-21801679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELenghttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21997/19459http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessOliveira, Mayara SetúbalAlmeida-Monteiro, Priscila Silva deNunes, Larissa TeixeiraLinhares, Francisco Renan AragãoPinheiro, João Paulo SilvaPinheiro, Romulo Roberto RibeiroFerreira, Filipe OliveiraCampello, Claudio CabralSalmito-Vanderley, Carminda Sandra Brito2022-12-01T11:30:41Zoai:ojs.pkp.sfu.ca:article/21997Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2022-12-01T11:30:41Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine Criopreservação do sêmen de tambaqui utilizando dry shipper e máquina de congelação programada |
title |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine |
spellingShingle |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine Oliveira, Mayara Setúbal Colossoma macropomum Conservation methods Extenders Sperm class analyzer Teleost. Colossoma macropomum Diluidores Métodos de conservação Sperm class analyzer. Oliveira, Mayara Setúbal Colossoma macropomum Conservation methods Extenders Sperm class analyzer Teleost. Colossoma macropomum Diluidores Métodos de conservação Sperm class analyzer. |
title_short |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine |
title_full |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine |
title_fullStr |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine |
title_full_unstemmed |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine |
title_sort |
Cryopreservation of tambaqui semen using a dry shipper and a programmed freezing machine |
author |
Oliveira, Mayara Setúbal |
author_facet |
Oliveira, Mayara Setúbal Oliveira, Mayara Setúbal Almeida-Monteiro, Priscila Silva de Nunes, Larissa Teixeira Linhares, Francisco Renan Aragão Pinheiro, João Paulo Silva Pinheiro, Romulo Roberto Ribeiro Ferreira, Filipe Oliveira Campello, Claudio Cabral Salmito-Vanderley, Carminda Sandra Brito Almeida-Monteiro, Priscila Silva de Nunes, Larissa Teixeira Linhares, Francisco Renan Aragão Pinheiro, João Paulo Silva Pinheiro, Romulo Roberto Ribeiro Ferreira, Filipe Oliveira Campello, Claudio Cabral Salmito-Vanderley, Carminda Sandra Brito |
author_role |
author |
author2 |
Almeida-Monteiro, Priscila Silva de Nunes, Larissa Teixeira Linhares, Francisco Renan Aragão Pinheiro, João Paulo Silva Pinheiro, Romulo Roberto Ribeiro Ferreira, Filipe Oliveira Campello, Claudio Cabral Salmito-Vanderley, Carminda Sandra Brito |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Mayara Setúbal Almeida-Monteiro, Priscila Silva de Nunes, Larissa Teixeira Linhares, Francisco Renan Aragão Pinheiro, João Paulo Silva Pinheiro, Romulo Roberto Ribeiro Ferreira, Filipe Oliveira Campello, Claudio Cabral Salmito-Vanderley, Carminda Sandra Brito |
dc.subject.por.fl_str_mv |
Colossoma macropomum Conservation methods Extenders Sperm class analyzer Teleost. Colossoma macropomum Diluidores Métodos de conservação Sperm class analyzer. |
topic |
Colossoma macropomum Conservation methods Extenders Sperm class analyzer Teleost. Colossoma macropomum Diluidores Métodos de conservação Sperm class analyzer. |
description |
Tambaqui (Colossoma macropomum) is a native freshwater fish that is of great importance for Brazilian aquaculture. Because of this importance, several techniques have been developed to improve the reproduction of this species in captivity. One of these techniques is the cryopreservation of sperm. In an effort to increase the efficiency of cryopreservation protocols, researchers have tried to determine suitable diluting solutions and freezing methods, which will provide a better post-thaw sperm quality. Thus, this study aimed to evaluate the efficiency of different diluents and freezing methods for the cryopreservation of tambaqui (C. macropomum) sperm. Samples of fresh semen were diluted in different treatments (Glucose 5% + 10% Dimethyl sulfoxide – DMSO, Glucose 5% + 10% Methyl glycol – MG, BTS + 10% DMSO and BTS + 10% MG) at a 1:9 dilution rate and frozen in a programmed freezing machine and a dry shipper. The semen samples were thawed and evaluated for vitality, sperm morphology and kinetics. Cryopreserved semen with DMSO and using the programmed freezing machine provided a greater percentage of motile sperm (15.44 ± 1.04%) after thawing compared to the dry shipper (3.99 ± 0.55%), regardless of the diluent. Additionally, DMSO showed better sperm velocities than MG regardless of the freezing method and the extender employed. A higher percentage of living spermatozoa was observed when glucose (37.28 ± 1.32%) (regardless of the freezing method and cryoprotectant) and DMSO (37.98 ± 1.25%) was used in the programmed freezing machine. For morphology, a greater amount of normal spermatozoa (46.10 ± 1.82%) was observed when the semen was cryopreserved using a freezing machine programmed with DMSO as the cryoprotectant and Glucose or BTS (38.16 ± 1.9% and 39.26 ± 1.87%, respectively) as extenders. Therefore, we suggest the use of the DMSO (10%) cryoprotectant in association with the Glucose (5%) extended in the programmed freezing machine for cryopreservation of C. macropomum semen. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Empírica de Campo |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21997 10.5433/1679-0359.2016v37n4p2167 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21997 |
identifier_str_mv |
10.5433/1679-0359.2016v37n4p2167 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/21997/19459 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 37 No. 4 (2016); 2167-2180 Semina: Ciências Agrárias; v. 37 n. 4 (2016); 2167-2180 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
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1822182751312281600 |
dc.identifier.doi.none.fl_str_mv |
10.5433/1679-0359.2016v37n4p2167 |