Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition

Detalhes bibliográficos
Autor(a) principal: Reis, Cândida Camila dos
Data de Publicação: 2015
Outros Autores: Henrique, Douglas Sampaio, Schervinski, Edimara, Zanela, Juliano, Constantino, Leonel Vinicius, Dallo, Rafael
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15264
Resumo: This study aims to evaluate different techniques for determination of soluble sugars (fraction CA) in foods used for ruminant nutrition. Feed analyzed were: sugar-cane, bermuda grass, corn meal and soybean meal. Dry matter (DM), ash, ether extract (EE) and crude protein (CP) were determined to make possible the calculation of total carbohydrates concentration in the samples. The soluble carbohydrate fraction were determined in 15 repetitions of each sample by two different analytical techniques. One technique based on soluble carbohydrates extraction and its quantification by spectrophotometry after chelation of the sugar with anthrone; and another one that uses phenol as the chelating agent. The experiment were conducted in a completely randomized design and the data submitted to the two-factor factorial analysis of variance (? = 0.05), with the different feeds and the two techniques as factors. There was no statistical difference between techniques, but the effect of the feed and the interaction feed x technique were significant. So, a new analysis of variance was conducted to test the difference between techniques in each feed separately. Only soybean meal did not show statistical difference between the water soluble sugars determined by the two techniques.
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spelling Comparison of techniques for determination of soluble sugars used in feed for ruminant nutritionComparação entre técnicas para determinação de açúcares solúveis em alimentos utilizados na nutrição de ruminantesChemical analysisCarbohydrate fractionsFeeding systems.Análises bromatológicasFracionamento de carboidratosSistemas de alimentação.Nutrição de RuminantesThis study aims to evaluate different techniques for determination of soluble sugars (fraction CA) in foods used for ruminant nutrition. Feed analyzed were: sugar-cane, bermuda grass, corn meal and soybean meal. Dry matter (DM), ash, ether extract (EE) and crude protein (CP) were determined to make possible the calculation of total carbohydrates concentration in the samples. The soluble carbohydrate fraction were determined in 15 repetitions of each sample by two different analytical techniques. One technique based on soluble carbohydrates extraction and its quantification by spectrophotometry after chelation of the sugar with anthrone; and another one that uses phenol as the chelating agent. The experiment were conducted in a completely randomized design and the data submitted to the two-factor factorial analysis of variance (? = 0.05), with the different feeds and the two techniques as factors. There was no statistical difference between techniques, but the effect of the feed and the interaction feed x technique were significant. So, a new analysis of variance was conducted to test the difference between techniques in each feed separately. Only soybean meal did not show statistical difference between the water soluble sugars determined by the two techniques.O objetivo do presente trabalho foi avaliar duas técnicas para determinação de açúcares solúveis (fração CA) em alimentos utilizados na nutrição de ruminantes. Os alimentos analisados foram: cana-de-açúcar, capim estrela, milho moído e farelo de soja. Foi determinado o conteúdo de matéria seca (MS), matéria mineral (MM), extrato etéreo (EE) e proteína bruta (PB), para calcular a concentração de carboidratos totais das amostras. O teor de carboidratos solúveis foi determinado em 15 repetições de cada amostra, utilizando-se duas técnicas analíticas distintas: uma que se baseia na extração dos carboidratos solúveis e na suaquantificação por espectrofotometria após a complexação do açúcar com a antrona; e outra que consiste na utilização do fenol como agente complexador. O delineamento utilizado foi inteiramente casualizado e os dados foram submetidos à análise de variância bifatorial (? = 0,05), sendo os fatores, os diferentes alimentos e as duas técnicas. Não houve diferença significativa para o fator técnica, mas houve efeito do fator alimento e para a interação alimento x técnica. Por isso, foram realizadas novas análises de variância para testar se havia diferença entre as técnicas em cada um dos alimentos. Apenas o farelo de soja não apresentou diferença significativa quanto ao teor de açúcar solúvel determinado pelas duas técnicas. UEL2015-02-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Empírica de Campoapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1526410.5433/1679-0359.2015v36n1p401Semina: Ciências Agrárias; Vol. 36 No. 1 (2015); 401-408Semina: Ciências Agrárias; v. 36 n. 1 (2015); 401-4081679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15264/pdf_595Copyright (c) 2015 Semina: Ciências Agráriashttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessReis, Cândida Camila dosHenrique, Douglas SampaioSchervinski, EdimaraZanela, JulianoConstantino, Leonel ViniciusDallo, Rafael2023-01-13T15:26:29Zoai:ojs.pkp.sfu.ca:article/15264Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2023-01-13T15:26:29Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
Comparação entre técnicas para determinação de açúcares solúveis em alimentos utilizados na nutrição de ruminantes
title Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
spellingShingle Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
Reis, Cândida Camila dos
Chemical analysis
Carbohydrate fractions
Feeding systems.
Análises bromatológicas
Fracionamento de carboidratos
Sistemas de alimentação.
Nutrição de Ruminantes
title_short Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
title_full Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
title_fullStr Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
title_full_unstemmed Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
title_sort Comparison of techniques for determination of soluble sugars used in feed for ruminant nutrition
author Reis, Cândida Camila dos
author_facet Reis, Cândida Camila dos
Henrique, Douglas Sampaio
Schervinski, Edimara
Zanela, Juliano
Constantino, Leonel Vinicius
Dallo, Rafael
author_role author
author2 Henrique, Douglas Sampaio
Schervinski, Edimara
Zanela, Juliano
Constantino, Leonel Vinicius
Dallo, Rafael
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Reis, Cândida Camila dos
Henrique, Douglas Sampaio
Schervinski, Edimara
Zanela, Juliano
Constantino, Leonel Vinicius
Dallo, Rafael
dc.subject.por.fl_str_mv Chemical analysis
Carbohydrate fractions
Feeding systems.
Análises bromatológicas
Fracionamento de carboidratos
Sistemas de alimentação.
Nutrição de Ruminantes
topic Chemical analysis
Carbohydrate fractions
Feeding systems.
Análises bromatológicas
Fracionamento de carboidratos
Sistemas de alimentação.
Nutrição de Ruminantes
description This study aims to evaluate different techniques for determination of soluble sugars (fraction CA) in foods used for ruminant nutrition. Feed analyzed were: sugar-cane, bermuda grass, corn meal and soybean meal. Dry matter (DM), ash, ether extract (EE) and crude protein (CP) were determined to make possible the calculation of total carbohydrates concentration in the samples. The soluble carbohydrate fraction were determined in 15 repetitions of each sample by two different analytical techniques. One technique based on soluble carbohydrates extraction and its quantification by spectrophotometry after chelation of the sugar with anthrone; and another one that uses phenol as the chelating agent. The experiment were conducted in a completely randomized design and the data submitted to the two-factor factorial analysis of variance (? = 0.05), with the different feeds and the two techniques as factors. There was no statistical difference between techniques, but the effect of the feed and the interaction feed x technique were significant. So, a new analysis of variance was conducted to test the difference between techniques in each feed separately. Only soybean meal did not show statistical difference between the water soluble sugars determined by the two techniques.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Pesquisa Empírica de Campo
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15264
10.5433/1679-0359.2015v36n1p401
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15264
identifier_str_mv 10.5433/1679-0359.2015v36n1p401
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/15264/pdf_595
dc.rights.driver.fl_str_mv Copyright (c) 2015 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Semina: Ciências Agrárias
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 36 No. 1 (2015); 401-408
Semina: Ciências Agrárias; v. 36 n. 1 (2015); 401-408
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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