Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band

Detalhes bibliográficos
Autor(a) principal: Leonhardt, Creusa Maria Rodrigues
Data de Publicação: 2006
Outros Autores: Caetano, Lucia Giuliano, Fenocchio, Alberto, Caetano Filho, Mauro, Leonhardt, Julio Hermann
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2537
Resumo: 14 specimens of Nile’s tilapia were analyzed cytogenetically, Oreochromis niloticus, that belong to the stock of fish breeding from the Freshwater Aquaculture Station of the Londrina State University in the Paraná, Brazil. All specimens presented the same disploid number of 44 chromosomes. The NORs were observed in four chromosomes with marks in terminal position of the short arm and the hybridization “in situ” (FISH) with probe of 18 S also evidenced the presence of two pairs of chromosomes containing ribbosomic cistrons. The treatment with the fluochromes CMA3 and DAPI, respectively, didn’t show shinning bands in any chromosome of the complement. The band C (CBG) evidenced regions of heterochromatin distributed on several chromosomes in the centromeric regions, being observed some marks in telomeric regions, mainly on the biggest pair of chromosomes of the complement, a pair presented itself almost totally heterochromatic. The obtained results are in accordance with the data found in literature, nevertheless when the C bands and NORs were analyzed, were evidenced some differences that apparently characterized the local fish population of the Londrina State University. Key words: Cytogenetics, Oreochromis niloticus, NORs, CMA3, DAPI, fish, heterochromatin.
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spelling Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes bandCaracterização cariotípica de um estoque de tilápia do Nilo,Oreochromis niloticus, da Universidade Estadual de Londrina, mediante diversas técnicas de bandamento cromossômicoCytogeneticsOreochromis niloticusNORsCMA3DAPIFishHeterochromatin.CitogenéticaOreochromis niloticusNORsCMA 3DAPIHeterocromatina.14 specimens of Nile’s tilapia were analyzed cytogenetically, Oreochromis niloticus, that belong to the stock of fish breeding from the Freshwater Aquaculture Station of the Londrina State University in the Paraná, Brazil. All specimens presented the same disploid number of 44 chromosomes. The NORs were observed in four chromosomes with marks in terminal position of the short arm and the hybridization “in situ” (FISH) with probe of 18 S also evidenced the presence of two pairs of chromosomes containing ribbosomic cistrons. The treatment with the fluochromes CMA3 and DAPI, respectively, didn’t show shinning bands in any chromosome of the complement. The band C (CBG) evidenced regions of heterochromatin distributed on several chromosomes in the centromeric regions, being observed some marks in telomeric regions, mainly on the biggest pair of chromosomes of the complement, a pair presented itself almost totally heterochromatic. The obtained results are in accordance with the data found in literature, nevertheless when the C bands and NORs were analyzed, were evidenced some differences that apparently characterized the local fish population of the Londrina State University. Key words: Cytogenetics, Oreochromis niloticus, NORs, CMA3, DAPI, fish, heterochromatin.Foram analisados citogeneticamente 14 indivíduos de tilápia do Nilo, Oreochromis niloticus que fazem parte do estoque de reposição de reprodutores da Estação de Piscicultura da Universidade Estadual de Londrina, Paraná, Brasil. Todos os indivíduos apresentaram o mesmo número diplóide de 44 cromossomos. As NORs foram observadas em quatro cromossomos com marcações em posição terminal do braço curto e a hibridação “in situ” (FISH) com sonda de 18S também evidenciou a presença de dois pares de cromossomos contendo cístrons ribossômicos. O tratamento com os fluorocromos CMA3 e DAPI, respectivamente, não mostrou bandas brilhantes em nenhum cromossomo do complemento. A Banda C (CBG) evidenciou, regiões de heterocromatina distribuídas em vários cromossomos nas regiões centroméricas, sendo observadas algumas marcações em regiões teloméricas, principalmente no maior par de cromossomos do complemento, um par apresentou-se quase totalmente heterocromático. Os resultados obtidos estão de acordo com os dados disponíveis na literatura, porém, quando analisadas as bandas C e as NORs, foram evidenciadas algumas diferenças que aparentemente caracterizam a população local de peixes da Universidade Estadual de Londrina.UEL2006-07-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/253710.5433/1679-0359.2006v27n4p679Semina: Ciências Agrárias; Vol. 27 No. 4 (2006); 679-684Semina: Ciências Agrárias; v. 27 n. 4 (2006); 679-6841679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2537/2174Leonhardt, Creusa Maria RodriguesCaetano, Lucia GiulianoFenocchio, AlbertoCaetano Filho, MauroLeonhardt, Julio Hermanninfo:eu-repo/semantics/openAccess2010-03-17T17:57:35Zoai:ojs.pkp.sfu.ca:article/2537Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2010-03-17T17:57:35Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
Caracterização cariotípica de um estoque de tilápia do Nilo,Oreochromis niloticus, da Universidade Estadual de Londrina, mediante diversas técnicas de bandamento cromossômico
title Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
spellingShingle Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
Leonhardt, Creusa Maria Rodrigues
Cytogenetics
Oreochromis niloticus
NORs
CMA3
DAPI
Fish
Heterochromatin.
Citogenética
Oreochromis niloticus
NORs
CMA 3
DAPI
Heterocromatina.
title_short Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
title_full Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
title_fullStr Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
title_full_unstemmed Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
title_sort Karyotypical characterization from stock of Nile tilapia, Oreochromis niloticus, at Londrina State University, PR, Brazil, through several techniques of chromosomes band
author Leonhardt, Creusa Maria Rodrigues
author_facet Leonhardt, Creusa Maria Rodrigues
Caetano, Lucia Giuliano
Fenocchio, Alberto
Caetano Filho, Mauro
Leonhardt, Julio Hermann
author_role author
author2 Caetano, Lucia Giuliano
Fenocchio, Alberto
Caetano Filho, Mauro
Leonhardt, Julio Hermann
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Leonhardt, Creusa Maria Rodrigues
Caetano, Lucia Giuliano
Fenocchio, Alberto
Caetano Filho, Mauro
Leonhardt, Julio Hermann
dc.subject.por.fl_str_mv Cytogenetics
Oreochromis niloticus
NORs
CMA3
DAPI
Fish
Heterochromatin.
Citogenética
Oreochromis niloticus
NORs
CMA 3
DAPI
Heterocromatina.
topic Cytogenetics
Oreochromis niloticus
NORs
CMA3
DAPI
Fish
Heterochromatin.
Citogenética
Oreochromis niloticus
NORs
CMA 3
DAPI
Heterocromatina.
description 14 specimens of Nile’s tilapia were analyzed cytogenetically, Oreochromis niloticus, that belong to the stock of fish breeding from the Freshwater Aquaculture Station of the Londrina State University in the Paraná, Brazil. All specimens presented the same disploid number of 44 chromosomes. The NORs were observed in four chromosomes with marks in terminal position of the short arm and the hybridization “in situ” (FISH) with probe of 18 S also evidenced the presence of two pairs of chromosomes containing ribbosomic cistrons. The treatment with the fluochromes CMA3 and DAPI, respectively, didn’t show shinning bands in any chromosome of the complement. The band C (CBG) evidenced regions of heterochromatin distributed on several chromosomes in the centromeric regions, being observed some marks in telomeric regions, mainly on the biggest pair of chromosomes of the complement, a pair presented itself almost totally heterochromatic. The obtained results are in accordance with the data found in literature, nevertheless when the C bands and NORs were analyzed, were evidenced some differences that apparently characterized the local fish population of the Londrina State University. Key words: Cytogenetics, Oreochromis niloticus, NORs, CMA3, DAPI, fish, heterochromatin.
publishDate 2006
dc.date.none.fl_str_mv 2006-07-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2537
10.5433/1679-0359.2006v27n4p679
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2537
identifier_str_mv 10.5433/1679-0359.2006v27n4p679
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2537/2174
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 27 No. 4 (2006); 679-684
Semina: Ciências Agrárias; v. 27 n. 4 (2006); 679-684
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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