Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria

Detalhes bibliográficos
Autor(a) principal: Carpentieri-Pípolo, Valéria
Data de Publicação: 2008
Outros Autores: Gallo-Meagher, Maria, Dickson, Don W., Gorbet, Daniel W., Mendes, Maria de Lurdes, Souza, Silvia Graciele Hülse de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Semina. Ciências Agrárias (Online)
Texto Completo: https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2693
Resumo: This  work  had  the  aim  of  comparing  the  method  of  hybridization  with  digoxigenin  and the chemiluminescent detection  system with  the method of  radioactive  labeled. The cultivars used were susceptible Florunner  and  resistant COAN. The genomic DNA was  isolated  from young  leaves  and Southern blotting analysis was conducted using RFLP probe R2430E. The detection of the probe labeled with digoxigenin was as sensitive as labeled by the radioactivity, however with advantages above the method  radioactive. Due  to be a  safe method and  to present  reduced costs,  the demarcation method based on the digoxigenin and chemiluminescent detection is highly recommendable.
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spelling Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenariaComparação entre métodos de marcação da sonda de RFLP R2430E utilizada na seleção de cultivares de amendoim resistente à Meloidogyne arenariaNonradiactive detectionDigoxigeninMolecular markers.Detecção não radioativaDigoxigeninaMarcadores moleculares. This  work  had  the  aim  of  comparing  the  method  of  hybridization  with  digoxigenin  and the chemiluminescent detection  system with  the method of  radioactive  labeled. The cultivars used were susceptible Florunner  and  resistant COAN. The genomic DNA was  isolated  from young  leaves  and Southern blotting analysis was conducted using RFLP probe R2430E. The detection of the probe labeled with digoxigenin was as sensitive as labeled by the radioactivity, however with advantages above the method  radioactive. Due  to be a  safe method and  to present  reduced costs,  the demarcation method based on the digoxigenin and chemiluminescent detection is highly recommendable.Neste trabalho foram comparados o método de hibridização com digoxigenina e o sistema de detecção por quimioluminescência com o método de marcação radioativa. Foram utilizados o cultivar suscetível Florunner e o resistente COAN. O DNA genômico foi isolado de folhas jovens e a análise de Southern blotting  foi  conduzida  utilizando  a  sonda  de  RFLP  R2430E. A  detecção  da  sonda  marcada  com digoxigenina foi tão sensível quanto àquela marcada pela radioatividadade, porém com vantagens sobre o método radioativo. Por ser um método seguro e apresentar custos reduzidos, o método de marcação baseado na digoxigenina e detecção por quimioluminescência é altamente recomendável.UEL2008-08-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/269310.5433/1679-0359.2008v29n4p783Semina: Ciências Agrárias; Vol. 29 No. 4 (2008); 783-788Semina: Ciências Agrárias; v. 29 n. 4 (2008); 783-7881679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2693/2339Carpentieri-Pípolo, ValériaGallo-Meagher, MariaDickson, Don W.Gorbet, Daniel W.Mendes, Maria de LurdesSouza, Silvia Graciele Hülse deinfo:eu-repo/semantics/openAccess2015-11-19T18:38:41Zoai:ojs.pkp.sfu.ca:article/2693Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:38:41Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false
dc.title.none.fl_str_mv Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
Comparação entre métodos de marcação da sonda de RFLP R2430E utilizada na seleção de cultivares de amendoim resistente à Meloidogyne arenaria
title Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
spellingShingle Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
Carpentieri-Pípolo, Valéria
Nonradiactive detection
Digoxigenin
Molecular markers.
Detecção não radioativa
Digoxigenina
Marcadores moleculares.
title_short Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
title_full Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
title_fullStr Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
title_full_unstemmed Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
title_sort Comparison between methods of demarcation of probe of RFLP R2430E used in the selection of resistance of peanut to Meloidogyne arenaria
author Carpentieri-Pípolo, Valéria
author_facet Carpentieri-Pípolo, Valéria
Gallo-Meagher, Maria
Dickson, Don W.
Gorbet, Daniel W.
Mendes, Maria de Lurdes
Souza, Silvia Graciele Hülse de
author_role author
author2 Gallo-Meagher, Maria
Dickson, Don W.
Gorbet, Daniel W.
Mendes, Maria de Lurdes
Souza, Silvia Graciele Hülse de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Carpentieri-Pípolo, Valéria
Gallo-Meagher, Maria
Dickson, Don W.
Gorbet, Daniel W.
Mendes, Maria de Lurdes
Souza, Silvia Graciele Hülse de
dc.subject.por.fl_str_mv Nonradiactive detection
Digoxigenin
Molecular markers.
Detecção não radioativa
Digoxigenina
Marcadores moleculares.
topic Nonradiactive detection
Digoxigenin
Molecular markers.
Detecção não radioativa
Digoxigenina
Marcadores moleculares.
description This  work  had  the  aim  of  comparing  the  method  of  hybridization  with  digoxigenin  and the chemiluminescent detection  system with  the method of  radioactive  labeled. The cultivars used were susceptible Florunner  and  resistant COAN. The genomic DNA was  isolated  from young  leaves  and Southern blotting analysis was conducted using RFLP probe R2430E. The detection of the probe labeled with digoxigenin was as sensitive as labeled by the radioactivity, however with advantages above the method  radioactive. Due  to be a  safe method and  to present  reduced costs,  the demarcation method based on the digoxigenin and chemiluminescent detection is highly recommendable.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2693
10.5433/1679-0359.2008v29n4p783
url https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2693
identifier_str_mv 10.5433/1679-0359.2008v29n4p783
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/2693/2339
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv UEL
publisher.none.fl_str_mv UEL
dc.source.none.fl_str_mv Semina: Ciências Agrárias; Vol. 29 No. 4 (2008); 783-788
Semina: Ciências Agrárias; v. 29 n. 4 (2008); 783-788
1679-0359
1676-546X
reponame:Semina. Ciências Agrárias (Online)
instname:Universidade Estadual de Londrina (UEL)
instacron:UEL
instname_str Universidade Estadual de Londrina (UEL)
instacron_str UEL
institution UEL
reponame_str Semina. Ciências Agrárias (Online)
collection Semina. Ciências Agrárias (Online)
repository.name.fl_str_mv Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)
repository.mail.fl_str_mv semina.agrarias@uel.br
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