Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Semina. Ciências Agrárias (Online) |
Texto Completo: | https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12023 |
Resumo: | The aim study it had as objective to evaluate the effect of different ammonia levels, on the parameters of sperm motility, the artificial fertilization and the early development of jundia (Rhamdia quelen; Siluriformes, Heptapteridae). The semen was activated using the dilution of 1:70 (semen:water) and evaluated by the CASA method. It was considered as treatment water contending 0,06; 0,28; 0,50; 0,77 and 0,84 mg of total ammonia.water-1 L, so for sperm activation as for fertilization and incubation of the oocytes. The incubation was composed for a recirculation system of 250L for each treatment, with four incubators, being that each incubator approximately had useful volume of 2,5L with 3,800 eggs. Had been evaluated the parameters of sperm motility, fertilization rate, thermal unit accumulated for beginning and ending of the hatching, hatching rate, embryonic and percentile development of normal larvae. The results had been submitted the analysis of variance and the analysis of regression to a 5% of probability level. The ammonia levels gifts in the water did not interfere on the evaluated parameters (p> 0,05), except about to the early embryonic development, where the embryos displayed to the biggest levels of contamination for ammonia had presented greaters (p>0,05) closing of the blastopore rates. In general way, the presence of until 0,84mg of total ammonia. Water-1 L speeds up the embryonic early development but they do not interfere with the fertilization and hatching rates. |
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Semina. Ciências Agrárias (Online) |
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Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae)Efeito da amônia sobre a fertilização artificial de ovócitos e no desenvolvimento inicial do jundiá cinza (Rhamdia quelen; Siluriformes, Heptapteridae)AmmoniaEmbryoLarvaeFishPollutant.AmôniaEmbriãoLarvasPeixePoluente.AquiculturaLimnologiaToxicologiaThe aim study it had as objective to evaluate the effect of different ammonia levels, on the parameters of sperm motility, the artificial fertilization and the early development of jundia (Rhamdia quelen; Siluriformes, Heptapteridae). The semen was activated using the dilution of 1:70 (semen:water) and evaluated by the CASA method. It was considered as treatment water contending 0,06; 0,28; 0,50; 0,77 and 0,84 mg of total ammonia.water-1 L, so for sperm activation as for fertilization and incubation of the oocytes. The incubation was composed for a recirculation system of 250L for each treatment, with four incubators, being that each incubator approximately had useful volume of 2,5L with 3,800 eggs. Had been evaluated the parameters of sperm motility, fertilization rate, thermal unit accumulated for beginning and ending of the hatching, hatching rate, embryonic and percentile development of normal larvae. The results had been submitted the analysis of variance and the analysis of regression to a 5% of probability level. The ammonia levels gifts in the water did not interfere on the evaluated parameters (p> 0,05), except about to the early embryonic development, where the embryos displayed to the biggest levels of contamination for ammonia had presented greaters (p>0,05) closing of the blastopore rates. In general way, the presence of until 0,84mg of total ammonia. Water-1 L speeds up the embryonic early development but they do not interfere with the fertilization and hatching rates.O presente estudo teve como objetivo avaliar os efeitos de diferentes níveis de amônia, sobre os parâmetros de motilidade espermática, a fertilização artificial e o desenvolvimento inicial do jundiá cinza (Rhamdia quelen; Siluriformes, Heptapteridae). O sêmen foi ativado utilizando a diluição de 1:70 (sêmen:água) e avaliado pelo método CASA. Foi considerado como tratamento água contendo 0,06; 0,28; 0,50; 0,77 e 0,84 mg de amônia total.L de água-1, tanto para ativação espermática quanto para fertilização e incubação dos ovócitos. A incubação era composta por um sistema de recirculação de 250L para cada tratamento, com quatro incubadoras, sendo que cada incubadora tinha volume útil de 2,5L com aproximadamente 3.800 ovos. Foram avaliados os parâmetros de motilidade espermática, de taxa de fertilização, unidade térmica acumulada para início e término da eclosão, taxa de eclosão, desenvolvimento embrionário e percentual de larvas normais. Os resultados foram submetidos a análise de variância e a análise de regressão a um nível de 5% de probabilidade. Os níveis de amônia presentes na água não interferiram sobre os parâmetros avaliados (p>0,05), exceto quanto ao desenvolvimento embrionário inicial, onde os embriões expostos aos maiores níveis de contaminação por amônia apresentaram maiores (p<0,05) taxas de fechamento do blastóporo. De modo geral, a presença de até 0,84mg de amônia total.L de água-1 acelera o desenvolvimento inicial embrionário mas não interferem nas taxas de fertilização e eclosão.UEL2013-10-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPesquisa Históricaapplication/pdfhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/1202310.5433/1679-0359.2013v34n5p2447Semina: Ciências Agrárias; Vol. 34 No. 5 (2013); 2447-2456Semina: Ciências Agrárias; v. 34 n. 5 (2013); 2447-24561679-03591676-546Xreponame:Semina. Ciências Agrárias (Online)instname:Universidade Estadual de Londrina (UEL)instacron:UELporhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12023/pdf_44Vidal, EléxioBastos, Denise Nascimento deOliveira, Edna AparecidaButzge, Arno JulianoMewes, Juliana KasperBaumgartner, GilmarSanches, Paulo VanderleiBombardelli, Robie Allaninfo:eu-repo/semantics/openAccess2015-11-19T18:36:26Zoai:ojs.pkp.sfu.ca:article/12023Revistahttp://www.uel.br/revistas/uel/index.php/semagrariasPUBhttps://ojs.uel.br/revistas/uel/index.php/semagrarias/oaisemina.agrarias@uel.br1679-03591676-546Xopendoar:2015-11-19T18:36:26Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL)false |
dc.title.none.fl_str_mv |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) Efeito da amônia sobre a fertilização artificial de ovócitos e no desenvolvimento inicial do jundiá cinza (Rhamdia quelen; Siluriformes, Heptapteridae) |
title |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) |
spellingShingle |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) Vidal, Eléxio Ammonia Embryo Larvae Fish Pollutant. Amônia Embrião Larvas Peixe Poluente. Aquicultura Limnologia Toxicologia |
title_short |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) |
title_full |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) |
title_fullStr |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) |
title_full_unstemmed |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) |
title_sort |
Effect of ammonia on the artificial fertilization of oocytes and early development of jundia (Rhamdia quelen, Siluriformes, Heptapteridae) |
author |
Vidal, Eléxio |
author_facet |
Vidal, Eléxio Bastos, Denise Nascimento de Oliveira, Edna Aparecida Butzge, Arno Juliano Mewes, Juliana Kasper Baumgartner, Gilmar Sanches, Paulo Vanderlei Bombardelli, Robie Allan |
author_role |
author |
author2 |
Bastos, Denise Nascimento de Oliveira, Edna Aparecida Butzge, Arno Juliano Mewes, Juliana Kasper Baumgartner, Gilmar Sanches, Paulo Vanderlei Bombardelli, Robie Allan |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Vidal, Eléxio Bastos, Denise Nascimento de Oliveira, Edna Aparecida Butzge, Arno Juliano Mewes, Juliana Kasper Baumgartner, Gilmar Sanches, Paulo Vanderlei Bombardelli, Robie Allan |
dc.subject.por.fl_str_mv |
Ammonia Embryo Larvae Fish Pollutant. Amônia Embrião Larvas Peixe Poluente. Aquicultura Limnologia Toxicologia |
topic |
Ammonia Embryo Larvae Fish Pollutant. Amônia Embrião Larvas Peixe Poluente. Aquicultura Limnologia Toxicologia |
description |
The aim study it had as objective to evaluate the effect of different ammonia levels, on the parameters of sperm motility, the artificial fertilization and the early development of jundia (Rhamdia quelen; Siluriformes, Heptapteridae). The semen was activated using the dilution of 1:70 (semen:water) and evaluated by the CASA method. It was considered as treatment water contending 0,06; 0,28; 0,50; 0,77 and 0,84 mg of total ammonia.water-1 L, so for sperm activation as for fertilization and incubation of the oocytes. The incubation was composed for a recirculation system of 250L for each treatment, with four incubators, being that each incubator approximately had useful volume of 2,5L with 3,800 eggs. Had been evaluated the parameters of sperm motility, fertilization rate, thermal unit accumulated for beginning and ending of the hatching, hatching rate, embryonic and percentile development of normal larvae. The results had been submitted the analysis of variance and the analysis of regression to a 5% of probability level. The ammonia levels gifts in the water did not interfere on the evaluated parameters (p> 0,05), except about to the early embryonic development, where the embryos displayed to the biggest levels of contamination for ammonia had presented greaters (p>0,05) closing of the blastopore rates. In general way, the presence of until 0,84mg of total ammonia. Water-1 L speeds up the embryonic early development but they do not interfere with the fertilization and hatching rates. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Pesquisa Histórica |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12023 10.5433/1679-0359.2013v34n5p2447 |
url |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12023 |
identifier_str_mv |
10.5433/1679-0359.2013v34n5p2447 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.uel.br/revistas/uel/index.php/semagrarias/article/view/12023/pdf_44 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
UEL |
publisher.none.fl_str_mv |
UEL |
dc.source.none.fl_str_mv |
Semina: Ciências Agrárias; Vol. 34 No. 5 (2013); 2447-2456 Semina: Ciências Agrárias; v. 34 n. 5 (2013); 2447-2456 1679-0359 1676-546X reponame:Semina. Ciências Agrárias (Online) instname:Universidade Estadual de Londrina (UEL) instacron:UEL |
instname_str |
Universidade Estadual de Londrina (UEL) |
instacron_str |
UEL |
institution |
UEL |
reponame_str |
Semina. Ciências Agrárias (Online) |
collection |
Semina. Ciências Agrárias (Online) |
repository.name.fl_str_mv |
Semina. Ciências Agrárias (Online) - Universidade Estadual de Londrina (UEL) |
repository.mail.fl_str_mv |
semina.agrarias@uel.br |
_version_ |
1799306067180519424 |