EuroCollins® como meio de estocagem de dentes avulsionados : estudo in vitro da viabilidade celular

• Autor(a) principal: Botelho, Juliana Nunes
• Data de Publicação: 2010
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
• Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/2113
• topic: Avulsão dentária; Reimplante dentário; EuroCollins®; Meio de estocagem; Viabilidade celular; Azul de tripan; MTT, EuroCollins®; Células mononucleares do sangue periférico humano, EuroCollins®; Ligamento periodontal; Dente humano extraído, EuroCollins®; Ligamento periodontal; Cultura celular; Brasil.; Euro-Collins®; Viability; Tooth avulsion; Tooth reimplantation; Brazil.; Ciências da Saúde; Odontologia

The success of post-avulsion dental reimplantation is dependent on, among other factors, the condition of the periodontal ligament. Several media are recommended for storage of avulsed teeth. Euro-Collins® (EC) is a solution for gravitational perfusion and hypothermic storage of organs for donation. lts characteristics called the attention for its possible use as avulsed teeth storage medium. Thus, the aim of this work was to verify the effectiveness of EC in relation to other media traditionally used to avulsed teeth storage. The in vitro study tested EC comparing it to ultrapasteurized whole milk, Hank?s balanced salt solution (HBSS) and Minimum Essential Medium (MEM) as positive controls; and distilled water as negative control. The celI viability was analyzed by both: the trypan blue staining exclusion method and the tetrazolium-based colorimetric assay (MTT). Human peripheral blood mononuclear cells and human periodontal ligament (PDL) cells from extracted teeth and culture were incubated at 1, 3, 6, 12, and 24h. Also, variations in the conditions of EC use, such as temperature (25°C and 4°C) and days after opening and handling (ECO, EC3O, ECI2O or ECI8O) were tested. As for the trypan blue study, the human mononuclear cells incubated in ECO, EC3O and ECI2O showed different celI viability values than positive controls (p>O.05) only at 24h time. For human PDL cells from extracted teeth, ECO, milk, and HBSS were higher (p<O.O5) than EC3O and EC18O at 25°C. At 4°C, ECO showed higher results (p<O.05) than EC3O and ECI8O. The cultured human PDL celis incubated in ECO, EC3O, and EC18O showed lower results (p<O.05) than milk and HBSS at 25°C, and lower results (p<O05) than milk at 4°C at 24h time. As for MTT, the cultured human PDL cells incubated in ECO, EC3O, and ECI8O showed lower values than positive controis (p<O.05) since 1h and similar values in relation to distillated water from 12h (p>O.05) on at 25°C. As for 4°C, the absorbance values of ECO, EC3O and ECI8O were lower (p<O.O5) than milk since 1h, and in relation to HBSS (p<O,05) at 24h. The ECO, EC3O, EC18O, and MEM became lower than HBSS at 24h time (p<O.O5). The results suggest that, in our experimental conditions, the EC maintained the effectiveness similar to HBSS, traditionally used as avulsed teeth storage medium, until 12h at 4°C.