Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
Texto Completo: | http://repositorio.uem.br:8080/jspui/handle/1/1243 |
Resumo: | The objective of this study was to evaluate the potential of extracts derived from medicinal plants (Rosmarinus officinalis, Equisetum arvense and Moringa oleifera) and commercial products (based on plant extracts, vegetable oils and potassium phosphite) in vitro control and greenhouse of Diplocarpon rosae and evaluate through resistance induction of determining the specific activity of oxidative enzymes, catalase and polyphenol in phytopathogen and roses. In vitro evaluated the effect of treatments on the pathogen by mycelial growth rate index, inhibition of mycelial growth inhibition of sporulation, sporulation rate, protein synthesis, peroxidase and catalase specific activity using aqueous crude extract (ACE) to R. officinalis leaves (1%); ACE E. arvense stem (1%); ACE M. oleifera seeds (1%); commercial product based on fermented plant extracts (0.01%); commercial product based on potassium phosphite (0.01%); commercial product based on vegetable oils (0.01%); Ecolife® (0.01%); Thiophanate methyl fungicide basis (0.007%); adjuvant (0.0025%) on potato dextrose agar (PDA) and potato dextrose (BD). Under greenhouse conditions were evaluated incidence and severity of disease black spot, productivity (number of buttons), biometrics (length buttons, length and stem thickness), chlorophyll content and peroxidase, catalase and polyphenol oxidase specific activity, using the same treatments with ten times higher doses and the control was distilled water. In vitro results with commercial product based on vegetable oils 56% reduced mycelial growth; ACE E. arvense and commercial product based on fermented plant extracts inhibited 81 and 75% sporulation. The treatments with ACE R. officinalis, E. arvense, M. oleifera, commercial product based on vegetable oils and Ecolife® increased protein synthesis in D.rosae mycelium. Also in the mycelium was increased specific activity of peroxidase and catalase in ACE R. officinalis and commercial product based on potassium phosphite, respectively. In a greenhouse the largest stem length was observed in the treatment with the commercial product based on potassium phosphite and the greatest thickness of the stem in the three commercial products. The highest enzyme activities were observed in the peroxidase enzyme with a commercial product based on potassium phosphite in the first application, catalase, ACE M. oleifera and potassium phosphite on first application and polyphenol with ACE R. officinalis and M. oleifera in the third application. Natural products can be used in disease control blight and resistance induction, showing promising results. |
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Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseirasPlant extracts and commercial products in control of Diplocarpon rosae and resistance induction in rosesRosas de cortePinta pretaControle alternativoDiplocarpon rosaeProdutos naturaisExtratos vegetaisAtividade antifúngica.Alternative controlBlack spotCut rosesBrazil.Ciências AgráriasAgronomiaThe objective of this study was to evaluate the potential of extracts derived from medicinal plants (Rosmarinus officinalis, Equisetum arvense and Moringa oleifera) and commercial products (based on plant extracts, vegetable oils and potassium phosphite) in vitro control and greenhouse of Diplocarpon rosae and evaluate through resistance induction of determining the specific activity of oxidative enzymes, catalase and polyphenol in phytopathogen and roses. In vitro evaluated the effect of treatments on the pathogen by mycelial growth rate index, inhibition of mycelial growth inhibition of sporulation, sporulation rate, protein synthesis, peroxidase and catalase specific activity using aqueous crude extract (ACE) to R. officinalis leaves (1%); ACE E. arvense stem (1%); ACE M. oleifera seeds (1%); commercial product based on fermented plant extracts (0.01%); commercial product based on potassium phosphite (0.01%); commercial product based on vegetable oils (0.01%); Ecolife® (0.01%); Thiophanate methyl fungicide basis (0.007%); adjuvant (0.0025%) on potato dextrose agar (PDA) and potato dextrose (BD). Under greenhouse conditions were evaluated incidence and severity of disease black spot, productivity (number of buttons), biometrics (length buttons, length and stem thickness), chlorophyll content and peroxidase, catalase and polyphenol oxidase specific activity, using the same treatments with ten times higher doses and the control was distilled water. In vitro results with commercial product based on vegetable oils 56% reduced mycelial growth; ACE E. arvense and commercial product based on fermented plant extracts inhibited 81 and 75% sporulation. The treatments with ACE R. officinalis, E. arvense, M. oleifera, commercial product based on vegetable oils and Ecolife® increased protein synthesis in D.rosae mycelium. Also in the mycelium was increased specific activity of peroxidase and catalase in ACE R. officinalis and commercial product based on potassium phosphite, respectively. In a greenhouse the largest stem length was observed in the treatment with the commercial product based on potassium phosphite and the greatest thickness of the stem in the three commercial products. The highest enzyme activities were observed in the peroxidase enzyme with a commercial product based on potassium phosphite in the first application, catalase, ACE M. oleifera and potassium phosphite on first application and polyphenol with ACE R. officinalis and M. oleifera in the third application. Natural products can be used in disease control blight and resistance induction, showing promising results.O objetivo deste trabalho foi verificar o potencial de extratos oriundos de plantas medicinais (Rosmarinus officinalis, Equisetum arvense e Moringa oleifera) e produtos comerciais (a base de extratos vegetais, óleos vegetais e fosfito de potássio) no controle in vitro e em casa de vegetação de Diplocarpon rosae e avaliar a indução de resistência por meio da determinação da atividade específica das enzimas peroxidase, catalase e polifenoloxidase no fitopatógeno e em roseiras. In vitro avaliou-se o efeito dos tratamentos sobre o fitopatógeno pelo índice de velocidade de crescimento micelial, inibição do crescimento micelial, inibição da esporulação, índice de esporulação, síntese de proteínas, atividade específica de peroxidase e catalase utilizando extrato bruto aquoso (EBA) de folhas de R. officinalis (1%); EBA de caules de E. arvense (1%); EBA de sementes de M. oleifera (1%); produto comercial a base de extratos vegetais fermentados (0,01%); produto comercial a base de fosfito de potássio (0,01%); produto comercial a base de óleos vegetais (0,01%); Ecolife® (0,01%); fungicida a base de Tiofanato metílico (0,007%); adjuvante (0,0025%) e meio batata dextrose ágar (BDA testemunha) e batata-dextrose (BD testemunha). Em casa de vegetação foram avaliadas incidência e severidade da doença pinta preta, produtividade (número de botões), biometria (comprimento de botões, comprimento e espessura de haste), teor de clorofila e atividade específica de peroxidase, catalase e polifenoloxidase, utilizando os mesmo tratamentos com as doses dez vezes maiores, e a testemunha foi água destilada. O resultado in vitro com produto comercial a base de óleos vegetais reduziu 56% o crescimento micelial; EBA de E. arvense e produto comercial a base de extratos vegetais fermentados inibiram 81 e 75% a esporulação. Os tratamentos com EBA de R. officinalis, EBA de E. arvense, EBA de M. oleifera, produto comercial a base de óleos vegetais e Ecolife® aumentaram a síntese de proteínas no micélio D.rosae. Também no micélio houve aumento da atividade específica das enzimas peroxidase e catalase, no EBA de R. officinalis e produto comercial a base de fosfito de potássio, respectivamente. Em casa de vegetação o maior comprimento de haste foi observado no tratamento com produto comercial a base de fosfito de potássio e a maior espessura da haste nos três produtos comerciais. As maiores atividades específicas foram observadas na enzima peroxidase com produto comercial a base de fosfito de potássio na primeira aplicação, catalase, com EBA de M. oleifera e fosfito de potássio, na primeira aplicação e polifenoloxidase com EBA de R. officinalis e M. oleifera na terceira aplicação. Com os resultados observados, verifica-se que os produtos naturais podem ser utilizados no controle da doença pinta preta e na indução de resistência, apresentando resultados promissores.ix, 54 fUniversidade Estadual de Maringá. Centro de Ciências AgráriasBrasilPrograma de Pós-Graduação em AgronomiaUEMMaringá, PRDepartamento de AgronomiaKatia Regina Freitas Schwan-EstradaAdriana Terumi Itako - Universidade Federal de Santa CatarinaArmey Eduardo do Amaral Ecker - UEMSimon, Janaina Miyashiro2018-04-04T19:53:42Z2018-04-04T19:53:42Z2016info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://repositorio.uem.br:8080/jspui/handle/1/1243porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-04-04T19:53:42Zoai:localhost:1/1243Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:54:09.699703Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras Plant extracts and commercial products in control of Diplocarpon rosae and resistance induction in roses |
title |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras |
spellingShingle |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras Simon, Janaina Miyashiro Rosas de corte Pinta preta Controle alternativo Diplocarpon rosae Produtos naturais Extratos vegetais Atividade antifúngica. Alternative control Black spot Cut roses Brazil. Ciências Agrárias Agronomia |
title_short |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras |
title_full |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras |
title_fullStr |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras |
title_full_unstemmed |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras |
title_sort |
Extratos vegetais e produtos comerciais no controle de Diplocarpon rosae e na indução de resistência em roseiras |
author |
Simon, Janaina Miyashiro |
author_facet |
Simon, Janaina Miyashiro |
author_role |
author |
dc.contributor.none.fl_str_mv |
Katia Regina Freitas Schwan-Estrada Adriana Terumi Itako - Universidade Federal de Santa Catarina Armey Eduardo do Amaral Ecker - UEM |
dc.contributor.author.fl_str_mv |
Simon, Janaina Miyashiro |
dc.subject.por.fl_str_mv |
Rosas de corte Pinta preta Controle alternativo Diplocarpon rosae Produtos naturais Extratos vegetais Atividade antifúngica. Alternative control Black spot Cut roses Brazil. Ciências Agrárias Agronomia |
topic |
Rosas de corte Pinta preta Controle alternativo Diplocarpon rosae Produtos naturais Extratos vegetais Atividade antifúngica. Alternative control Black spot Cut roses Brazil. Ciências Agrárias Agronomia |
description |
The objective of this study was to evaluate the potential of extracts derived from medicinal plants (Rosmarinus officinalis, Equisetum arvense and Moringa oleifera) and commercial products (based on plant extracts, vegetable oils and potassium phosphite) in vitro control and greenhouse of Diplocarpon rosae and evaluate through resistance induction of determining the specific activity of oxidative enzymes, catalase and polyphenol in phytopathogen and roses. In vitro evaluated the effect of treatments on the pathogen by mycelial growth rate index, inhibition of mycelial growth inhibition of sporulation, sporulation rate, protein synthesis, peroxidase and catalase specific activity using aqueous crude extract (ACE) to R. officinalis leaves (1%); ACE E. arvense stem (1%); ACE M. oleifera seeds (1%); commercial product based on fermented plant extracts (0.01%); commercial product based on potassium phosphite (0.01%); commercial product based on vegetable oils (0.01%); Ecolife® (0.01%); Thiophanate methyl fungicide basis (0.007%); adjuvant (0.0025%) on potato dextrose agar (PDA) and potato dextrose (BD). Under greenhouse conditions were evaluated incidence and severity of disease black spot, productivity (number of buttons), biometrics (length buttons, length and stem thickness), chlorophyll content and peroxidase, catalase and polyphenol oxidase specific activity, using the same treatments with ten times higher doses and the control was distilled water. In vitro results with commercial product based on vegetable oils 56% reduced mycelial growth; ACE E. arvense and commercial product based on fermented plant extracts inhibited 81 and 75% sporulation. The treatments with ACE R. officinalis, E. arvense, M. oleifera, commercial product based on vegetable oils and Ecolife® increased protein synthesis in D.rosae mycelium. Also in the mycelium was increased specific activity of peroxidase and catalase in ACE R. officinalis and commercial product based on potassium phosphite, respectively. In a greenhouse the largest stem length was observed in the treatment with the commercial product based on potassium phosphite and the greatest thickness of the stem in the three commercial products. The highest enzyme activities were observed in the peroxidase enzyme with a commercial product based on potassium phosphite in the first application, catalase, ACE M. oleifera and potassium phosphite on first application and polyphenol with ACE R. officinalis and M. oleifera in the third application. Natural products can be used in disease control blight and resistance induction, showing promising results. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2018-04-04T19:53:42Z 2018-04-04T19:53:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.uem.br:8080/jspui/handle/1/1243 |
url |
http://repositorio.uem.br:8080/jspui/handle/1/1243 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Estadual de Maringá. Centro de Ciências Agrárias Brasil Programa de Pós-Graduação em Agronomia UEM Maringá, PR Departamento de Agronomia |
publisher.none.fl_str_mv |
Universidade Estadual de Maringá. Centro de Ciências Agrárias Brasil Programa de Pós-Graduação em Agronomia UEM Maringá, PR Departamento de Agronomia |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
collection |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) |
repository.name.fl_str_mv |
Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
|
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1813258637808762880 |