Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania

Detalhes bibliográficos
Autor(a) principal: Neitzke-Abreu, Herintha Coeto
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/2064
Resumo: The group of diseases collectively known as leishmaniasis is caused by protozoan parasites of the genus Leishmania, and these occur worldwide. Studies of the sandfly vectors are useful to assess the vulnerability of hosts in endemic regions, and to develop control measures. Laboratory methods should be evaluated to improve the diagnosis. This study evaluated the sandfly fauna, the natural infection rate of sandfly species by Leishmania, and the effects of environmental changes on the density of these insects. Also, the accuracy of the polymerase chain reaction (PCR) technique in diagnosing patients suspected of cutaneous leishmaniasis (CL) was estimated. An atypical case of CL is reported. Sandflies were collected in municipalities of the state of Paraná, by means of Falcão and Shannon traps, during severalstages of the study. Changes were made in the local environment (peridomiciles and animal shelters) between collections, in order to reduce the frequency of sandflies in the domiciles. Some female sandflies, after dissection and identification of the species, were subjected to multiplex PCR to detect Leishmania (Viannia). Samples from patients suspected of CL were examined by means of PCR, to detect Leishmania (Viannia). Subsequently to the environmental changes, the sandflies were collected mainly in domestic-animal shelters. Although none of the dissected females showed flagellates, DNA of Leishmania (Viannia) was detected by multiplex PCR in 0.46% of the specimens of Nyssomyia neivai and in 1.12% of the specimens of Nyssomyia whitmani. Among patients with CL, 83.87%, 51.67%, 64.52%, 85.71% and 96.10% were positive in the PCR of lesion (PCR-L), PCR blood (PCR-B), directparasite search, Indirect Immunofluorescence and Montenegro skin test, respectively. In five patients only the PCR-L was positive, and in two other patients only the PCR-B was positive. It is important to keep the peridomestic environment clean in order to prevent the formation of sandfly breeding sites. Ny. neivai and Ny. whitmani are susceptible to infection byLeishmania. The multiplex PCR technique, because of its specificity, sensitivity and feasibility, can be used in epidemiological studies to assess natural infection rates. PCR-L is indicated in patients with chronic lesions or reinfection with Leishmania, which can progress to mucosal lesion. PCR-B is indicated in patients with negative results from conventional tests, or for patients without lesions or with atypical lesions.
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spelling Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de LeishmaniaCutaneous leishmaniasis: influence of environmental changes on the frequency of sandflies and detection of LeishmaniaLeishmaniaLeishmaniose tegumentarFlebotomíneoPolymerase Chain Reaction (PCR)Brasil.Cutaneous leishmaniasisLeishmaniaSandfliesPCRBrazil.Ciências da SaúdeMedicinaThe group of diseases collectively known as leishmaniasis is caused by protozoan parasites of the genus Leishmania, and these occur worldwide. Studies of the sandfly vectors are useful to assess the vulnerability of hosts in endemic regions, and to develop control measures. Laboratory methods should be evaluated to improve the diagnosis. This study evaluated the sandfly fauna, the natural infection rate of sandfly species by Leishmania, and the effects of environmental changes on the density of these insects. Also, the accuracy of the polymerase chain reaction (PCR) technique in diagnosing patients suspected of cutaneous leishmaniasis (CL) was estimated. An atypical case of CL is reported. Sandflies were collected in municipalities of the state of Paraná, by means of Falcão and Shannon traps, during severalstages of the study. Changes were made in the local environment (peridomiciles and animal shelters) between collections, in order to reduce the frequency of sandflies in the domiciles. Some female sandflies, after dissection and identification of the species, were subjected to multiplex PCR to detect Leishmania (Viannia). Samples from patients suspected of CL were examined by means of PCR, to detect Leishmania (Viannia). Subsequently to the environmental changes, the sandflies were collected mainly in domestic-animal shelters. Although none of the dissected females showed flagellates, DNA of Leishmania (Viannia) was detected by multiplex PCR in 0.46% of the specimens of Nyssomyia neivai and in 1.12% of the specimens of Nyssomyia whitmani. Among patients with CL, 83.87%, 51.67%, 64.52%, 85.71% and 96.10% were positive in the PCR of lesion (PCR-L), PCR blood (PCR-B), directparasite search, Indirect Immunofluorescence and Montenegro skin test, respectively. In five patients only the PCR-L was positive, and in two other patients only the PCR-B was positive. It is important to keep the peridomestic environment clean in order to prevent the formation of sandfly breeding sites. Ny. neivai and Ny. whitmani are susceptible to infection byLeishmania. The multiplex PCR technique, because of its specificity, sensitivity and feasibility, can be used in epidemiological studies to assess natural infection rates. PCR-L is indicated in patients with chronic lesions or reinfection with Leishmania, which can progress to mucosal lesion. PCR-B is indicated in patients with negative results from conventional tests, or for patients without lesions or with atypical lesions.O grupo de doenças conhecidas como leishmanioses são causadas por protozoários do gênero Leishmania e têm ampla distribuição mundial. Estudos de flebotomíneos permitem avaliar a vulnerabilidade da endemia e desenvolver medidas de controle. Métodos laboratoriais devem ser avaliados a fim de melhorar o diagnóstico. O objetivo foi avaliar a fauna de flebotomíneos, a taxa de infecção natural por Leishmania e os efeitos de alterações ambientais sobre a densidade desses insetos. Também foi estimar a acurácia da técnica de reação em cadeia da polimerase (PCR) em pacientes suspeitos de leishmaniose tegumentar (LT) e relatar um caso atípico de LT. Flebotomíneos foram coletados em municípios do Paraná, com armadilhas de Falcão e Shannon, em diversas etapas. Foram feitas alterações no ambiente (peridomicílios e abrigos de animais domésticos) entre as etapas de coletas a fim de reduzir a frequência de flebotomíneos nas residências. Algumas fêmeas de flebotomíneos, após a dissecção e identificação das espécies, foram submetidas à multiplex-PCR para detecção de Leishmania (Viannia). Nas amostras de pacientes suspeitos de LT foram realizadas a PCRpara detecção de Leishmania (Viannia). Após as alterações ambientais, os flebotomíneosforam coletados principalmente em abrigos de animais domésticos. Apesar de nenhuma das fêmeas dissecadas ter sido encontrada com flagelados, DNA de Leishmania (Viannia) foi detectado por multiplex-PCR em 0,46% de Nyssomyia neivai e 1,12% de Nyssomyia whitmani. Entre os pacientes com LT, 83,87%, 51,67%, 64,52%, 85,71% e 96,10% foram positivos na PCR de lesão (PCR-L), PCR de sangue (PCR-S), pesquisa direta do parasito, imunofluorescência indireta e intradermorreação de Montenegro, respectivamente. Em cinco pacientes, apenas a PCR-L foi positiva e em outros dois pacientes, apenas a PCR-S foi positiva. Observa-se a importância de manter o peridomicílio limpo, a fim de evitar a formação de criadouros de flebotomíneos. Ny. neivai e Ny. whitmani são susceptíveis à infecção por Leishmania. A multiplex-PCR, devido à sua especificidade, sensibilidade e viabilidade, pode ser usada em estudos epidemiológicos para avaliar taxas de infecção natural. A PCR-L é indicada em pacientes com lesões crônicas ou reinfecção por Leishmania, que pode evoluir para a forma mucosa. A PCR-S é indicada em pacientes com resultados negativos em testes convencionais ou para pacientes sem lesão ou com lesões atípicas.89 fUniversidade Estadual de MaringáBrasilPrograma de Pós-Graduação em Ciências da SaúdeUEMMaringá, PRCentro de Ciências da SaúdeMaria Valdrinez Campana LonardoniAnita Hilda Straus Takahashi - Universidade Federal de São PauloDennis Armando Bertolini - UEMIone Parra Barbosa Tessmann - UEMTerezinha Inez E. Svidzinski - UEMNeitzke-Abreu, Herintha Coeto2018-04-09T18:22:03Z2018-04-09T18:22:03Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://repositorio.uem.br:8080/jspui/handle/1/2064porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)instname:Universidade Estadual de Maringá (UEM)instacron:UEM2018-04-09T18:22:03Zoai:localhost:1/2064Repositório InstitucionalPUBhttp://repositorio.uem.br:8080/oai/requestopendoar:2024-04-23T14:55:05.945661Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
Cutaneous leishmaniasis: influence of environmental changes on the frequency of sandflies and detection of Leishmania
title Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
spellingShingle Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
Neitzke-Abreu, Herintha Coeto
Leishmania
Leishmaniose tegumentar
Flebotomíneo
Polymerase Chain Reaction (PCR)
Brasil.
Cutaneous leishmaniasis
Leishmania
Sandflies
PCR
Brazil.
Ciências da Saúde
Medicina
title_short Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
title_full Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
title_fullStr Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
title_full_unstemmed Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
title_sort Leishmaniose tegumentar : influência de alterações ambientais na frequência de flebotomíneos e detecção de Leishmania
author Neitzke-Abreu, Herintha Coeto
author_facet Neitzke-Abreu, Herintha Coeto
author_role author
dc.contributor.none.fl_str_mv Maria Valdrinez Campana Lonardoni
Anita Hilda Straus Takahashi - Universidade Federal de São Paulo
Dennis Armando Bertolini - UEM
Ione Parra Barbosa Tessmann - UEM
Terezinha Inez E. Svidzinski - UEM
dc.contributor.author.fl_str_mv Neitzke-Abreu, Herintha Coeto
dc.subject.por.fl_str_mv Leishmania
Leishmaniose tegumentar
Flebotomíneo
Polymerase Chain Reaction (PCR)
Brasil.
Cutaneous leishmaniasis
Leishmania
Sandflies
PCR
Brazil.
Ciências da Saúde
Medicina
topic Leishmania
Leishmaniose tegumentar
Flebotomíneo
Polymerase Chain Reaction (PCR)
Brasil.
Cutaneous leishmaniasis
Leishmania
Sandflies
PCR
Brazil.
Ciências da Saúde
Medicina
description The group of diseases collectively known as leishmaniasis is caused by protozoan parasites of the genus Leishmania, and these occur worldwide. Studies of the sandfly vectors are useful to assess the vulnerability of hosts in endemic regions, and to develop control measures. Laboratory methods should be evaluated to improve the diagnosis. This study evaluated the sandfly fauna, the natural infection rate of sandfly species by Leishmania, and the effects of environmental changes on the density of these insects. Also, the accuracy of the polymerase chain reaction (PCR) technique in diagnosing patients suspected of cutaneous leishmaniasis (CL) was estimated. An atypical case of CL is reported. Sandflies were collected in municipalities of the state of Paraná, by means of Falcão and Shannon traps, during severalstages of the study. Changes were made in the local environment (peridomiciles and animal shelters) between collections, in order to reduce the frequency of sandflies in the domiciles. Some female sandflies, after dissection and identification of the species, were subjected to multiplex PCR to detect Leishmania (Viannia). Samples from patients suspected of CL were examined by means of PCR, to detect Leishmania (Viannia). Subsequently to the environmental changes, the sandflies were collected mainly in domestic-animal shelters. Although none of the dissected females showed flagellates, DNA of Leishmania (Viannia) was detected by multiplex PCR in 0.46% of the specimens of Nyssomyia neivai and in 1.12% of the specimens of Nyssomyia whitmani. Among patients with CL, 83.87%, 51.67%, 64.52%, 85.71% and 96.10% were positive in the PCR of lesion (PCR-L), PCR blood (PCR-B), directparasite search, Indirect Immunofluorescence and Montenegro skin test, respectively. In five patients only the PCR-L was positive, and in two other patients only the PCR-B was positive. It is important to keep the peridomestic environment clean in order to prevent the formation of sandfly breeding sites. Ny. neivai and Ny. whitmani are susceptible to infection byLeishmania. The multiplex PCR technique, because of its specificity, sensitivity and feasibility, can be used in epidemiological studies to assess natural infection rates. PCR-L is indicated in patients with chronic lesions or reinfection with Leishmania, which can progress to mucosal lesion. PCR-B is indicated in patients with negative results from conventional tests, or for patients without lesions or with atypical lesions.
publishDate 2013
dc.date.none.fl_str_mv 2013
2018-04-09T18:22:03Z
2018-04-09T18:22:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.uem.br:8080/jspui/handle/1/2064
url http://repositorio.uem.br:8080/jspui/handle/1/2064
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Programa de Pós-Graduação em Ciências da Saúde
UEM
Maringá, PR
Centro de Ciências da Saúde
publisher.none.fl_str_mv Universidade Estadual de Maringá
Brasil
Programa de Pós-Graduação em Ciências da Saúde
UEM
Maringá, PR
Centro de Ciências da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
collection Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
repository.name.fl_str_mv Repositório Institucional da Universidade Estadual de Maringá (RI-UEM) - Universidade Estadual de Maringá (UEM)
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