Avaliação do processo de nitrificação em um filtro biológico aerado submerso alimentado com efluente de RALF

• Autor(a) principal: Oliveira, Laurence Damasceno de
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
• Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/3550
• topic: Reator (RALF); Pós-tratamento; Nitrificação; FISH; RALF; Maringá; Paraná; Brasil.; Nitrification; Post-treatment; FISH; RALF; Maringá; Paraná; Brazil.; Engenharias; Engenharia Civil

This study aimed to evaluate the post-treatment of a Reactor Anaerobic Sludge Mantle Flow and Ascendant (RALF) through a Submerged Aerated Filter Biological (FBAS) with emphasis on the process of studying the behavior of nitrification nitrifying population through hybridization technique Probes Fluorescent in situ hybridization (FISH), under different operating conditions. This study was conducted in an experimental unit consists of a FBAS (0.605 m³), filled with PET lids (252 m². m-3) followed by a secondary clarifier (1.56 m³) in three distinct stages of operation. The unit was installed shortly after RALF, which is part of the system of Sewage Treatment Station (STS)-administered by Southern Sanitation Company of the State of Paraná (SANEPAR), located in the city of Maringá- PR. The operation steps 1, 2 and 3 had a run time of 45, 60 and 45 days, respectively, with feed flow rate of 0.5 m³.h-1 in the first stage and 0.2 m³.h-1 second and third stage. We adopted the sludge recirculation rates of the secondary clarifier of 0.1 m³.h-1 for stage 1 and 0.2 m³.h-1 to stage 2 and 3. We sought to evaluate the nitrifying population dynamics in the upper part of the reactor detecting ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing (NOB) using oligonucleotide probes by FISH method. According to the results, application of organic loading decreasing rate (OLR) organic loading rate along the stages proposals resulted in better performance in the nitrification process where Step 3 showed an efficiency of 78% NH3-N removal, subjected to an OLR of 0.83 KgDBO.m-3.dia-1. The nitrifying community checked at the top of the reactor under different OLR was found to be different behaviors, where the Step 3 with the application of a OLR of 0.19 KgDBO.m- 3.dia-1, showed a larger cell number total labeled with DAPI in relation to the other steps of 35% Nitrosomona (NSM 156), 9% Nitrobacter (NIT 3) and 25% Nitrospira (NTSPA 662).