Espectroscopia fotoacústica para determinar a taxa de propagação e a distribuição de medicamentos na unha humana

• Autor(a) principal: Nuglisch, Leonice Elci Rehfeld
• Data de Publicação: 2004
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da Universidade Estadual de Maringá (RI-UEM)
• Texto Completo: http://repositorio.uem.br:8080/jspui/handle/1/2684
• topic: Espectroscopia fotoacústica; Medicamento; Perfil de penetração; Unha humana; Estudo "in vitro"; Absorção óptica; Agente anti-fúngico.; Ciências Exatas e da Terra; Física

The aim of the present study was to evaluate the penetration of two antifungal agents through human nail by using Photoacoustic Spectroscopy. The experiments were performed using two different substances provided by the Stiefel Laboratory, coded as G0291AC and G0291BL. The measurements were perfomed in "in vitro" in 15 healthy human nail provided by different volunteers. The study was designed to be performed in two different steps. In the first one we measured the nail thermal diffusivity for the first time using the so-called Open Photoacustic Cell and obtained the value (8 ± 1) x 10-4 cm2/s. After that we investigated whether the Photoacoustic Spectroscopy (PAS) can be used to determine both the penetration and the distribution profiles of the drugs through the nail thickness. After confirming this hypothesis, we investigated the best procedure to be used in the experiments and observed that by performing the spectrum illuminating the sample in their internal side, the opposite side where the drugs were applied, an exponential grow in the photoacoustic signal generated by the drugs was observed. The study was focused in terms of using the results to determine the propagation rate of these products. The statistical test "one way ANOVA" was used to discriminate the antifungal agents with higher penetration rate through the nails. The comparison between penetration rates of the two substances was performed and the results showed that G0291BL has the higher penetration rate. Finally, the results of this work showed the versatility of the Photoacoustic Spectroscopy to evaluate the penetration rate of substances through the human nail, indicating that this method may be useful in the study of the penetration and the distribution of a wide range of different drugs through the nail.