Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59497 |
Resumo: | Oeceoclades maculata is a terrestrial orchid species that has potential for commercial purposes. Taking that into consideration, the present investigation aimed at studying its germination and initial development in vitro as well as its acclimatization. The influence of Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) media in the presence and absence of 0.3% activated charcoal on in vitro germination and protocorm development were investigated. The effects of different concentrations of BA in combination with 0.5 mg L-1 NAA on seedling multiplication and growth were evaluated. The possibility of using dark-grown stem segments for micropropagation and acclimatization under laboratory and field conditions was also assessed. The results indicated that the most adequate media for germination were full-strength MS enriched with activated charcoal or KC supplemented with 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA. In terms of protocorm development, KC supplemented with 1.5 mg L-1 BA alone or in combination with 0.5 mg L-1 NAA provided the best results. The addition of 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA to KC medium favored the best results for seedling multiplication and development. The use of dark-grown stem segments is a viable alternative for the micropropagation of O. maculata. Regarding acclimatization, 100% survival of plants was observed during the initial phases and under field conditions average survival was 53.33%. |
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Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environmentsGermination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environmentsactivated charcoal; culture media; etiolation; orchid protocorm development.activated charcoal; culture media; etiolation; orchid protocorm development.Oeceoclades maculata is a terrestrial orchid species that has potential for commercial purposes. Taking that into consideration, the present investigation aimed at studying its germination and initial development in vitro as well as its acclimatization. The influence of Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) media in the presence and absence of 0.3% activated charcoal on in vitro germination and protocorm development were investigated. The effects of different concentrations of BA in combination with 0.5 mg L-1 NAA on seedling multiplication and growth were evaluated. The possibility of using dark-grown stem segments for micropropagation and acclimatization under laboratory and field conditions was also assessed. The results indicated that the most adequate media for germination were full-strength MS enriched with activated charcoal or KC supplemented with 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA. In terms of protocorm development, KC supplemented with 1.5 mg L-1 BA alone or in combination with 0.5 mg L-1 NAA provided the best results. The addition of 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA to KC medium favored the best results for seedling multiplication and development. The use of dark-grown stem segments is a viable alternative for the micropropagation of O. maculata. Regarding acclimatization, 100% survival of plants was observed during the initial phases and under field conditions average survival was 53.33%.Oeceoclades maculata is a terrestrial orchid species that has potential for commercial purposes. Taking that into consideration, the present investigation aimed at studying its germination and initial development in vitro as well as its acclimatization. The influence of Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) media in the presence and absence of 0.3% activated charcoal on in vitro germination and protocorm development were investigated. The effects of different concentrations of BA in combination with 0.5 mg L-1 NAA on seedling multiplication and growth were evaluated. The possibility of using dark-grown stem segments for micropropagation and acclimatization under laboratory and field conditions was also assessed. The results indicated that the most adequate media for germination were full-strength MS enriched with activated charcoal or KC supplemented with 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA. In terms of protocorm development, KC supplemented with 1.5 mg L-1 BA alone or in combination with 0.5 mg L-1 NAA provided the best results. The addition of 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA to KC medium favored the best results for seedling multiplication and development. The use of dark-grown stem segments is a viable alternative for the micropropagation of O. maculata. Regarding acclimatization, 100% survival of plants was observed during the initial phases and under field conditions average survival was 53.33%.Universidade Estadual De Maringá2022-03-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5949710.4025/actascibiolsci.v44i1.59497Acta Scientiarum. Biological Sciences; Vol 44 (2022): Publicação contínua; e59497Acta Scientiarum. Biological Sciences; v. 44 (2022): Publicação contínua; e594971807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59497/751375153895Copyright (c) 2022 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessViana, Joyce Cavalcante Cerqueira, Paulo Rogério Alves Alves, Laís Ramos Arrais, Francisca das Chagas Leandro Suzuki, Rogério Mamoru Ferreira, Wagner de Melo2022-04-01T17:38:39Zoai:periodicos.uem.br/ojs:article/59497Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-04-01T17:38:39Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
title |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
spellingShingle |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments Viana, Joyce Cavalcante activated charcoal; culture media; etiolation; orchid protocorm development. activated charcoal; culture media; etiolation; orchid protocorm development. |
title_short |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
title_full |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
title_fullStr |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
title_full_unstemmed |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
title_sort |
Germination and initial development in vitro, and acclimatization of Oeceoclades maculata (Orchidaceae), aiming at its reintroduction in natural environments |
author |
Viana, Joyce Cavalcante |
author_facet |
Viana, Joyce Cavalcante Cerqueira, Paulo Rogério Alves Alves, Laís Ramos Arrais, Francisca das Chagas Leandro Suzuki, Rogério Mamoru Ferreira, Wagner de Melo |
author_role |
author |
author2 |
Cerqueira, Paulo Rogério Alves Alves, Laís Ramos Arrais, Francisca das Chagas Leandro Suzuki, Rogério Mamoru Ferreira, Wagner de Melo |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Viana, Joyce Cavalcante Cerqueira, Paulo Rogério Alves Alves, Laís Ramos Arrais, Francisca das Chagas Leandro Suzuki, Rogério Mamoru Ferreira, Wagner de Melo |
dc.subject.por.fl_str_mv |
activated charcoal; culture media; etiolation; orchid protocorm development. activated charcoal; culture media; etiolation; orchid protocorm development. |
topic |
activated charcoal; culture media; etiolation; orchid protocorm development. activated charcoal; culture media; etiolation; orchid protocorm development. |
description |
Oeceoclades maculata is a terrestrial orchid species that has potential for commercial purposes. Taking that into consideration, the present investigation aimed at studying its germination and initial development in vitro as well as its acclimatization. The influence of Murashige and Skoog (MS), Knudson C (KC), and Vacin and Went (VW) media in the presence and absence of 0.3% activated charcoal on in vitro germination and protocorm development were investigated. The effects of different concentrations of BA in combination with 0.5 mg L-1 NAA on seedling multiplication and growth were evaluated. The possibility of using dark-grown stem segments for micropropagation and acclimatization under laboratory and field conditions was also assessed. The results indicated that the most adequate media for germination were full-strength MS enriched with activated charcoal or KC supplemented with 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA. In terms of protocorm development, KC supplemented with 1.5 mg L-1 BA alone or in combination with 0.5 mg L-1 NAA provided the best results. The addition of 1.5 mg L-1 BA in combination with 0.5 mg L-1 NAA to KC medium favored the best results for seedling multiplication and development. The use of dark-grown stem segments is a viable alternative for the micropropagation of O. maculata. Regarding acclimatization, 100% survival of plants was observed during the initial phases and under field conditions average survival was 53.33%. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59497 10.4025/actascibiolsci.v44i1.59497 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59497 |
identifier_str_mv |
10.4025/actascibiolsci.v44i1.59497 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/59497/751375153895 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 44 (2022): Publicação contínua; e59497 Acta Scientiarum. Biological Sciences; v. 44 (2022): Publicação contínua; e59497 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317397991063552 |