Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463 |
Resumo: | The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds. |
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Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweedsmarine algaepolyglycanscoagulation teststhrombin.The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds. Universidade Estadual De Maringá2018-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/mswordhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3446310.4025/actascibiolsci.v40i1.34463Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e34463Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e344631807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463/751375145393Copyright (c) 2018 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessRodrigues, José Ariévilo GurgelBarcellos, Priscila GomesGlauser, Bianca FernandesQuinderé, Ana Luíza GomesBenevides, Norma Maria BarrosTovar, Ana Maria FreireMourão, Paulo Antônio de Souza2022-02-17T21:59:57Zoai:periodicos.uem.br/ojs:article/34463Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:59:57Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
title |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
spellingShingle |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds Rodrigues, José Ariévilo Gurgel marine algae polyglycans coagulation tests thrombin. |
title_short |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
title_full |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
title_fullStr |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
title_full_unstemmed |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
title_sort |
Extraction, physical-chemical characterization and in vitro inhibitory potential of thrombin generation of crude sulfated polysaccharides from Brazilian tropical seaweeds |
author |
Rodrigues, José Ariévilo Gurgel |
author_facet |
Rodrigues, José Ariévilo Gurgel Barcellos, Priscila Gomes Glauser, Bianca Fernandes Quinderé, Ana Luíza Gomes Benevides, Norma Maria Barros Tovar, Ana Maria Freire Mourão, Paulo Antônio de Souza |
author_role |
author |
author2 |
Barcellos, Priscila Gomes Glauser, Bianca Fernandes Quinderé, Ana Luíza Gomes Benevides, Norma Maria Barros Tovar, Ana Maria Freire Mourão, Paulo Antônio de Souza |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, José Ariévilo Gurgel Barcellos, Priscila Gomes Glauser, Bianca Fernandes Quinderé, Ana Luíza Gomes Benevides, Norma Maria Barros Tovar, Ana Maria Freire Mourão, Paulo Antônio de Souza |
dc.subject.por.fl_str_mv |
marine algae polyglycans coagulation tests thrombin. |
topic |
marine algae polyglycans coagulation tests thrombin. |
description |
The biotechnological value of macroalgae for screening assays of thrombin generation-TG using sulfated polysaccharides-SPs as substitutes to heparin has been poorly explored. Five Brazilian species of macroalgae (Gracilaria birdiae, Acanthophora muscoides, Halymenia sp., Caulerpa cupressoides and C. racemosa) were analyzed and compared for their abundance, physical-chemical characteristics and in vitro anticoagulant assays of activated partial thromboplastin time-APTT, prothrombin time-PT and TG. Papain extraction yielded (p < 0.001) from 0.66±0.03% (C. racemosa) to 41.60±1.10% (Halymenia sp.) of crude SPs varying sulfate (8.41-42.60%) and total sugars (47.80-70.53%). Crude SPs showed difference in mobility and resolution pattern by agarose electrophoresis, while polyacrylamide analysis revealed SPs of > 100 kDa. These procedures, combined with the use of Stains-All, also indicated nonSPs. APTTs ranged from 2.81 (A. muscoides) to 21.30 IU (Halymenia sp.) vs. heparin (193 IU), and were dependent on sulfation of the crude SPs. PT was not altered. With respect to TG assay, crude SPs modified concentration-dependent and independently from molecular mass TG by both intrinsic/extrinsic pathways in 60-fold diluted human plasma, with total intrinsic inactivation using crude SPs from A. muscoides in parallel to heparin (p < 0.05). Thrombosis in vitro is differentially modulated by distinct crude SPs from Brazilian seaweeds. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-03-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463 10.4025/actascibiolsci.v40i1.34463 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463 |
identifier_str_mv |
10.4025/actascibiolsci.v40i1.34463 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34463/751375145393 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Acta Scientiarum. Biological Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Acta Scientiarum. Biological Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/msword |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e34463 Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34463 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317396856504320 |