Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65188 |
Resumo: | The objective of this study was to evaluate the entomotoxic activity of the hydroalcoholic extract of Eragrostis plana Nees in an experimental model of cockroaches (Nauphoeta cinerea Olivier), for a possible bioinsecticide. For this purpose, aerial parts of E. plana were grounded to obtain the hydroalcoholic extract (EACA), after preparing the concentrations of (0.5; 2.5; 5.0; 12.5; 25.0; 50.0 100.0 μg g-1 per animal) a saline solution was used to control the treatment. To determine the receptors and pathways involved in responses to EACA doses of E. plana, a treatment with the drug octopamine and its inhibitor phentolamine were prepared. The effect of EACA on behavioral activities (grooming) and on cardiac activity were tested. All data were expressed as mean ± standard deviation, being analyzed by the two-way ANOVA test, followed by the Tukey test, considered significant when p<0.05. After carrying out the experiment, it was found that EACA induced a significant effect on leg grooming at all concentrations, except for the concentration of 100 μg g-1 per animal, but the same did not modulate antenna grooming. Regarding the receptors and pathways involved, it was observed that octopamine alone and together with the extract, had a significant effect on leg grooming, while phentolamine, together with the extract, influenced antennae grooming. Regarding the effect of the extract on the semi-isolated heart, it was noticed that the same doses that affected grooming, affected heart rate by decreasing it. At no concentration, at the end of the treatment, there was no recovery of the heart rate. The results point to a direct modulation of octopaminergic and cholinergic pathways in the insect nervous system. |
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Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea OlivierEntomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivierannoni-2 grass; grooming; semi-isolated heart; insecticidal activity.annoni-2 grass; grooming; semi-isolated heart; insecticidal activity.The objective of this study was to evaluate the entomotoxic activity of the hydroalcoholic extract of Eragrostis plana Nees in an experimental model of cockroaches (Nauphoeta cinerea Olivier), for a possible bioinsecticide. For this purpose, aerial parts of E. plana were grounded to obtain the hydroalcoholic extract (EACA), after preparing the concentrations of (0.5; 2.5; 5.0; 12.5; 25.0; 50.0 100.0 μg g-1 per animal) a saline solution was used to control the treatment. To determine the receptors and pathways involved in responses to EACA doses of E. plana, a treatment with the drug octopamine and its inhibitor phentolamine were prepared. The effect of EACA on behavioral activities (grooming) and on cardiac activity were tested. All data were expressed as mean ± standard deviation, being analyzed by the two-way ANOVA test, followed by the Tukey test, considered significant when p<0.05. After carrying out the experiment, it was found that EACA induced a significant effect on leg grooming at all concentrations, except for the concentration of 100 μg g-1 per animal, but the same did not modulate antenna grooming. Regarding the receptors and pathways involved, it was observed that octopamine alone and together with the extract, had a significant effect on leg grooming, while phentolamine, together with the extract, influenced antennae grooming. Regarding the effect of the extract on the semi-isolated heart, it was noticed that the same doses that affected grooming, affected heart rate by decreasing it. At no concentration, at the end of the treatment, there was no recovery of the heart rate. The results point to a direct modulation of octopaminergic and cholinergic pathways in the insect nervous system.The objective of this study was to evaluate the entomotoxic activity of the hydroalcoholic extract of Eragrostis plana Nees in an experimental model of cockroaches (Nauphoeta cinerea Olivier), for a possible bioinsecticide. For this purpose, aerial parts of E. plana were grounded to obtain the hydroalcoholic extract (EACA), after preparing the concentrations of (0.5; 2.5; 5.0; 12.5; 25.0; 50.0 100.0 μg g-1 per animal) a saline solution was used to control the treatment. To determine the receptors and pathways involved in responses to EACA doses of E. plana, a treatment with the drug octopamine and its inhibitor phentolamine were prepared. The effect of EACA on behavioral activities (grooming) and on cardiac activity were tested. All data were expressed as mean ± standard deviation, being analyzed by the two-way ANOVA test, followed by the Tukey test, considered significant when p<0.05. After carrying out the experiment, it was found that EACA induced a significant effect on leg grooming at all concentrations, except for the concentration of 100 μg g-1 per animal, but the same did not modulate antenna grooming. Regarding the receptors and pathways involved, it was observed that octopamine alone and together with the extract, had a significant effect on leg grooming, while phentolamine, together with the extract, influenced antennae grooming. Regarding the effect of the extract on the semi-isolated heart, it was noticed that the same doses that affected grooming, affected heart rate by decreasing it. At no concentration, at the end of the treatment, there was no recovery of the heart rate. The results point to a direct modulation of octopaminergic and cholinergic pathways in the insect nervous system.Universidade Estadual De Maringá2023-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6518810.4025/actascibiolsci.v45i1.65188Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e65188Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e651881807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65188/751375156611Copyright (c) 2023 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessAguiar, Angélica Vieira Pedroso, Lana Dorneles Borges, Bruna Trindade Vestena, Silvane2023-11-22T13:31:20Zoai:periodicos.uem.br/ojs:article/65188Revistahttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/PUBhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2023-11-22T13:31:20Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
title |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
spellingShingle |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier Aguiar, Angélica Vieira annoni-2 grass; grooming; semi-isolated heart; insecticidal activity. annoni-2 grass; grooming; semi-isolated heart; insecticidal activity. |
title_short |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
title_full |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
title_fullStr |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
title_full_unstemmed |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
title_sort |
Entomotoxic potential of hydroalcoholic extract of Eragrostis plana Nees in experimental model of Nauphoeta cinerea Olivier |
author |
Aguiar, Angélica Vieira |
author_facet |
Aguiar, Angélica Vieira Pedroso, Lana Dorneles Borges, Bruna Trindade Vestena, Silvane |
author_role |
author |
author2 |
Pedroso, Lana Dorneles Borges, Bruna Trindade Vestena, Silvane |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Aguiar, Angélica Vieira Pedroso, Lana Dorneles Borges, Bruna Trindade Vestena, Silvane |
dc.subject.por.fl_str_mv |
annoni-2 grass; grooming; semi-isolated heart; insecticidal activity. annoni-2 grass; grooming; semi-isolated heart; insecticidal activity. |
topic |
annoni-2 grass; grooming; semi-isolated heart; insecticidal activity. annoni-2 grass; grooming; semi-isolated heart; insecticidal activity. |
description |
The objective of this study was to evaluate the entomotoxic activity of the hydroalcoholic extract of Eragrostis plana Nees in an experimental model of cockroaches (Nauphoeta cinerea Olivier), for a possible bioinsecticide. For this purpose, aerial parts of E. plana were grounded to obtain the hydroalcoholic extract (EACA), after preparing the concentrations of (0.5; 2.5; 5.0; 12.5; 25.0; 50.0 100.0 μg g-1 per animal) a saline solution was used to control the treatment. To determine the receptors and pathways involved in responses to EACA doses of E. plana, a treatment with the drug octopamine and its inhibitor phentolamine were prepared. The effect of EACA on behavioral activities (grooming) and on cardiac activity were tested. All data were expressed as mean ± standard deviation, being analyzed by the two-way ANOVA test, followed by the Tukey test, considered significant when p<0.05. After carrying out the experiment, it was found that EACA induced a significant effect on leg grooming at all concentrations, except for the concentration of 100 μg g-1 per animal, but the same did not modulate antenna grooming. Regarding the receptors and pathways involved, it was observed that octopamine alone and together with the extract, had a significant effect on leg grooming, while phentolamine, together with the extract, influenced antennae grooming. Regarding the effect of the extract on the semi-isolated heart, it was noticed that the same doses that affected grooming, affected heart rate by decreasing it. At no concentration, at the end of the treatment, there was no recovery of the heart rate. The results point to a direct modulation of octopaminergic and cholinergic pathways in the insect nervous system. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-10-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65188 10.4025/actascibiolsci.v45i1.65188 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65188 |
identifier_str_mv |
10.4025/actascibiolsci.v45i1.65188 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/65188/751375156611 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e65188 Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e65188 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317390438170624 |