Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303 |
Resumo: | The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC50 = 455.7 µg mL-1. This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 µL mL-1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures. |
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Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activitiesnatural productsantioxidant assaysantimicrobialtrichomesThe aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC50 = 455.7 µg mL-1. This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 µL mL-1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures. Universidade Estadual De Maringá2016-10-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/mswordhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2830310.4025/actascibiolsci.v38i2.28303Acta Scientiarum. Biological Sciences; Vol 38 No 2 (2016); 139-147Acta Scientiarum. Biological Sciences; v. 38 n. 2 (2016); 139-1471807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303/751375143942Copyright (c) 2016 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessSantiago, Juliana AndradeCardoso, Maria das GraçasBatista, Luís RobertoCastro, Evaristo Mauro deTeixeira, Maria LuisaPires, Marinês Ferreira2022-02-20T22:00:46Zoai:periodicos.uem.br/ojs:article/28303Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-20T22:00:46Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
title |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
spellingShingle |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities Santiago, Juliana Andrade natural products antioxidant assays antimicrobial trichomes |
title_short |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
title_full |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
title_fullStr |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
title_full_unstemmed |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
title_sort |
Essential oil from Chenopodium ambrosioides L.: secretory structures, antibacterial and antioxidant activities |
author |
Santiago, Juliana Andrade |
author_facet |
Santiago, Juliana Andrade Cardoso, Maria das Graças Batista, Luís Roberto Castro, Evaristo Mauro de Teixeira, Maria Luisa Pires, Marinês Ferreira |
author_role |
author |
author2 |
Cardoso, Maria das Graças Batista, Luís Roberto Castro, Evaristo Mauro de Teixeira, Maria Luisa Pires, Marinês Ferreira |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Santiago, Juliana Andrade Cardoso, Maria das Graças Batista, Luís Roberto Castro, Evaristo Mauro de Teixeira, Maria Luisa Pires, Marinês Ferreira |
dc.subject.por.fl_str_mv |
natural products antioxidant assays antimicrobial trichomes |
topic |
natural products antioxidant assays antimicrobial trichomes |
description |
The aim of this study was to evaluate the antibacterial and antioxidant activities of essential oil from Chenopodium ambrosioides L. and to determine its secretory structures. The essential oil was extracted through the hydrodistillation technique using a modified Clevenger apparatus (2 hours) and chemically characterized by GC/MS and GC-FID. The antioxidant activity was determined by monitoring the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and by the oxidation of the β-carotene/linoleic acid system. The evaluation of antibacterial activity was performed by the agar cavity diffusion technique using the microorganisms Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella Cholerasuis. The characterization of trichomes was accomplished by Scanning Electron Microscopy (SEM) and histochemical tests with Nadi and Sudan IV reagents. The antioxidant activity demonstrated by the β-carotene/acid linoleic test, with IC50 = 455.7 µg mL-1. This oil also presented antibacterial activity for both Gram-negative and Gram-positive bacteria. The minimal inhibitory concentration ranged 62.5 to 250 µL mL-1. The presence of terpenes in the glandular trichomes was observed, suggesting that the essential oil is secreted by these structures. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-24 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303 10.4025/actascibiolsci.v38i2.28303 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303 |
identifier_str_mv |
10.4025/actascibiolsci.v38i2.28303 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/28303/751375143942 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2016 Acta Scientiarum. Biological Sciences info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2016 Acta Scientiarum. Biological Sciences |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/msword |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 38 No 2 (2016); 139-147 Acta Scientiarum. Biological Sciences; v. 38 n. 2 (2016); 139-147 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317391013838848 |