Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916 |
Resumo: | Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and >100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 µg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses. |
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Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generationUlvalescomplex glycanschemical analysisthrombosis. Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and >100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 µg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses. Universidade Estadual De Maringá2018-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documenthttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3491610.4025/actascibiolsci.v40i1.34916Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e34916Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e349161807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916/751375145527Copyright (c) 2018 Acta Scientiarum. Biological Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBarcellos, Priscila GomesRodrigues, José Ariévilo GurgelQueiroz, Ismael Nilo Lino deAraújo, Ianna Wivianne Fernandes deBenevides, Norma Maria BarrosMourão, Paulo Antônio de Souza2022-02-20T21:59:52Zoai:periodicos.uem.br/ojs:article/34916Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-20T21:59:52Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
title |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
spellingShingle |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation Barcellos, Priscila Gomes Ulvales complex glycans chemical analysis thrombosis. |
title_short |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
title_full |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
title_fullStr |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
title_full_unstemmed |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
title_sort |
Structural and physical-chemical analyses of sulfated polysaccharides from the sea lettuce Ulva lactuca and their effects on thrombin generation |
author |
Barcellos, Priscila Gomes |
author_facet |
Barcellos, Priscila Gomes Rodrigues, José Ariévilo Gurgel Queiroz, Ismael Nilo Lino de Araújo, Ianna Wivianne Fernandes de Benevides, Norma Maria Barros Mourão, Paulo Antônio de Souza |
author_role |
author |
author2 |
Rodrigues, José Ariévilo Gurgel Queiroz, Ismael Nilo Lino de Araújo, Ianna Wivianne Fernandes de Benevides, Norma Maria Barros Mourão, Paulo Antônio de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Barcellos, Priscila Gomes Rodrigues, José Ariévilo Gurgel Queiroz, Ismael Nilo Lino de Araújo, Ianna Wivianne Fernandes de Benevides, Norma Maria Barros Mourão, Paulo Antônio de Souza |
dc.subject.por.fl_str_mv |
Ulvales complex glycans chemical analysis thrombosis. |
topic |
Ulvales complex glycans chemical analysis thrombosis. |
description |
Ulva lactuca (Chlorophyceae) has biotechnologically-important sulfated-polysaccharides (Ul-SPs), but their potentials on thrombin generation (TG) are unknown. This study analyzed the structural and physical-chemical features of the Ul-SPs as modulators of TG. Proteolytic digestion yielded (13.13%) extract containing sulfate (20.43%) and total sugars (65.72%), besides ulvan consisting of rhamnose, xylose, glucose, glucuronic acid and α-/β-types glycosidic linkages as characterized by one-/two-dimensions nuclear magnetic resonance (NMR) experiments. Fractionation of the Ul-SPs by DEAE-cellulose chromatography yielded Ul-SP1 and Ul-SP2 (0.50 and 0.75 M NaCl, respectively) showing sulfation (15.72-18.04%) and total sugars (59.73-60.58%) consistent with the charge density pattern by combination of agarose/polyacrylamide gel eletrophoresis using sequential staining with toluidine blue and stains-all, although with slight differences in their sizes (40 and >100 kDa, respectively). By both activated partial thromboplastin time (APTT) and prothrombin time (PT) tests, anticoagulation of the fractions was virtually detected by APTT (0.39 and 0.43 IU, respectively) against heparin (193 IU). Fractions acted differently on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with 50% efficacies up to 8.3 µg, whereas at high concentrations suggested intrinsic hypercoagulability since heparin abolished both systems at low amounts. Ul-SPs block TG, but predicting thrombosis in increasing doses. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-30 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916 10.4025/actascibiolsci.v40i1.34916 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916 |
identifier_str_mv |
10.4025/actascibiolsci.v40i1.34916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916/pdf http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/34916/751375145527 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Acta Scientiarum. Biological Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Acta Scientiarum. Biological Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e34916 Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e34916 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317396918370304 |