Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L.
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119 |
Resumo: | This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the concentrations of 0.25 and 0.50 g L-1 at the 48-hour exposure, and the concentration of 0.75 g L-1, the two exposure times time significantly reduced (p < 0.05) the cell division rate. Importantly, all the three concentrations at the two exposure times tested caused cellular aberrations in significant numbers in this testing system. Therefore, under the conditions studied, the Ponceau 4R was cytotoxic. |
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Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L.azo dyecell divisioncellular aberrationscytotoxicityThis study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the concentrations of 0.25 and 0.50 g L-1 at the 48-hour exposure, and the concentration of 0.75 g L-1, the two exposure times time significantly reduced (p < 0.05) the cell division rate. Importantly, all the three concentrations at the two exposure times tested caused cellular aberrations in significant numbers in this testing system. Therefore, under the conditions studied, the Ponceau 4R was cytotoxic. Universidade Estadual De Maringá2015-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2311910.4025/actascibiolsci.v37i1.23119Acta Scientiarum. Biological Sciences; Vol 37 No 1 (2015); 101-106Acta Scientiarum. Biological Sciences; v. 37 n. 1 (2015); 101-1061807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119/pdf_42Santana, Gleuvânia MarquesSousa, Josefa Janaína do AnjosPeron, Ana Paulainfo:eu-repo/semantics/openAccess2016-11-21T09:10:31Zoai:periodicos.uem.br/ojs:article/23119Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2016-11-21T09:10:31Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
title |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
spellingShingle |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. Santana, Gleuvânia Marques azo dye cell division cellular aberrations cytotoxicity |
title_short |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
title_full |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
title_fullStr |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
title_full_unstemmed |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
title_sort |
Action of Ponceau 4R (E-124) food dye on root meristematic cells of Allium cepa L. |
author |
Santana, Gleuvânia Marques |
author_facet |
Santana, Gleuvânia Marques Sousa, Josefa Janaína do Anjos Peron, Ana Paula |
author_role |
author |
author2 |
Sousa, Josefa Janaína do Anjos Peron, Ana Paula |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Santana, Gleuvânia Marques Sousa, Josefa Janaína do Anjos Peron, Ana Paula |
dc.subject.por.fl_str_mv |
azo dye cell division cellular aberrations cytotoxicity |
topic |
azo dye cell division cellular aberrations cytotoxicity |
description |
This study aimed to evaluate the toxicity of Ponceau 4R food dye on the cell cycle in root meristematic cells of Allium cepa L. at three concentrations: 0.25, 0.50 and 0.75 g L-1, at exposure times of 24 and 48 hours. For each concentration, we used a set of five onion bulbs that were first rooted in distilled water and then transferred to their respective concentrations. Radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were mounted with the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5,000 cells for each control and exposure time. The calculated mitotic indices were subjected to the Chi-square test (p < 0.05). From the results, we observed that the concentrations of 0.25 and 0.50 g L-1 at the 48-hour exposure, and the concentration of 0.75 g L-1, the two exposure times time significantly reduced (p < 0.05) the cell division rate. Importantly, all the three concentrations at the two exposure times tested caused cellular aberrations in significant numbers in this testing system. Therefore, under the conditions studied, the Ponceau 4R was cytotoxic. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-10 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119 10.4025/actascibiolsci.v37i1.23119 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119 |
identifier_str_mv |
10.4025/actascibiolsci.v37i1.23119 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/23119/pdf_42 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 37 No 1 (2015); 101-106 Acta Scientiarum. Biological Sciences; v. 37 n. 1 (2015); 101-106 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
_version_ |
1799317396018692096 |