Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179

• Autor(a) principal: Santos, Michele Rocha dos
• Data de Publicação: 2013
• Outros Autores: Renesto, Erasmo
• Tipo de documento: Artigo
• Idioma: por; eng
• Título da fonte: Acta Scientiarum Biological Sciences
• DOI: 10.4025/actascibiolsci.v35i3.14179
• Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14179
• topic: allozyme; genetic diversity; heterozygosity; polymorphism; pisces

The genetic variability of Oligosarcus paranensis was estimated from a population collected in São Francisco river, Prudentópolis county in Paraná State (Brazil) using the electrophoresis in starch gel technique. Eleven enzymatic systems were analyzed: Aspartate aminotransaminase (AAT; E. C. 2.6.1), Alcohol dehydrogenase (ADH; E. C. 1.1.1.1), Esterase (EST; E. C. 3.1.1.1), Glucose-6-phosphate isomerase (GPI; E. C. 5.3.1.9), Glycerol-3-Phosphate dehydrogenase (G3PDH; E. C. 1.1.1), Isocitrate dehydrogenase (IDH; E. C. 1.1.1.42), L-lactate dehydrogenase (LDH; E. C. 1.1.1.27), Malate dehydrogenase (MDH; E. C. 1.1.1.37 ), Malate dehydrogenase NADP (ME; E. C. 1.1.1.40), Phosphoglucomutase (PGM; E. C. 5.4.2.2) and Sorbitol dehydrogenase (SORB; E.C. 1.1.1.14). Twenty loci were identified through 15% corn starch gel electrophoresis of which nine (45%) were polymorphic. The average expected heterozygosity was estimated as 0.1229 ± 0.1728, and the observed was 0.0586 ± 0.1069, indicating high genetic variability. The average value of FIS = 0.5145 indicates homozygote excess.