Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard

Detalhes bibliográficos
Autor(a) principal: De Conti, Daniela
Data de Publicação: 2018
Outros Autores: Corredor-Prado, Jenny Paola, Roecker Junior, Djalma, Suzuki, Rogério Mamoru, Guerra, Miguel Pedro, Pescador, Rosete
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Scientiarum Biological Sciences
Texto Completo: http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874
Resumo:  Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones and leaf primordia formation. Sucrose, fructose and glucose presence in this study is related to cell signaling. Thus, hormonal signals and carbohydrates alter metabolism, triggering PLBs initiation and development in C. tigrina. 
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spelling Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richardorchidhormonessugarsmorphogenesis. Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones and leaf primordia formation. Sucrose, fructose and glucose presence in this study is related to cell signaling. Thus, hormonal signals and carbohydrates alter metabolism, triggering PLBs initiation and development in C. tigrina. Universidade Estadual De Maringá2018-03-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/3787410.4025/actascibiolsci.v40i1.37874Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e37874Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e378741807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874/pdfCopyright (c) 2018 Acta Scientiarum. Biological Sciencesinfo:eu-repo/semantics/openAccessDe Conti, DanielaCorredor-Prado, Jenny PaolaRoecker Junior, DjalmaSuzuki, Rogério MamoruGuerra, Miguel PedroPescador, Rosete2022-02-17T21:59:59Zoai:periodicos.uem.br/ojs:article/37874Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:59:59Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false
dc.title.none.fl_str_mv Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
title Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
spellingShingle Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
De Conti, Daniela
orchid
hormones
sugars
morphogenesis.
title_short Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
title_full Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
title_fullStr Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
title_full_unstemmed Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
title_sort Determination of endogenous IAA and carbohydrates during the induction and development of protocorm-like bodies of Cattleya tigrina A. Richard
author De Conti, Daniela
author_facet De Conti, Daniela
Corredor-Prado, Jenny Paola
Roecker Junior, Djalma
Suzuki, Rogério Mamoru
Guerra, Miguel Pedro
Pescador, Rosete
author_role author
author2 Corredor-Prado, Jenny Paola
Roecker Junior, Djalma
Suzuki, Rogério Mamoru
Guerra, Miguel Pedro
Pescador, Rosete
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv De Conti, Daniela
Corredor-Prado, Jenny Paola
Roecker Junior, Djalma
Suzuki, Rogério Mamoru
Guerra, Miguel Pedro
Pescador, Rosete
dc.subject.por.fl_str_mv orchid
hormones
sugars
morphogenesis.
topic orchid
hormones
sugars
morphogenesis.
description  Tissue culture techniques have been employed for orchid mass propagation by means of the morphogenetic route of protocorm like-bodies (PLBs). This study aimed to analyze and compare Indole-acetic acid (IAA) and sugar endogenous levels in protocorm-like bodies (PLBs) induction and development in Cattleya tigrina, in order to better understand this process and to optimize micropropagation procedure protocols. Leaves grown on MS (Murashige and Skoog) culture medium, suplemented with 9 μM TDZ for PLBs induction and development were collected after 0, 2, 7, 14, 30, 60 and 100 days of cultivation, for further analysis. Increase of IAA and reduction of sugar levels are strongly related to morphogenetic response, that is, PLBs formation over the preexisting ones and leaf primordia formation. Sucrose, fructose and glucose presence in this study is related to cell signaling. Thus, hormonal signals and carbohydrates alter metabolism, triggering PLBs initiation and development in C. tigrina. 
publishDate 2018
dc.date.none.fl_str_mv 2018-03-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874
10.4025/actascibiolsci.v40i1.37874
url http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874
identifier_str_mv 10.4025/actascibiolsci.v40i1.37874
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/37874/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2018 Acta Scientiarum. Biological Sciences
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Acta Scientiarum. Biological Sciences
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual De Maringá
publisher.none.fl_str_mv Universidade Estadual De Maringá
dc.source.none.fl_str_mv Acta Scientiarum. Biological Sciences; Vol 40 (2018): Publicação Contínua; e37874
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e37874
1807-863X
1679-9283
reponame:Acta Scientiarum Biological Sciences
instname:Universidade Estadual de Maringá (UEM)
instacron:UEM
instname_str Universidade Estadual de Maringá (UEM)
instacron_str UEM
institution UEM
reponame_str Acta Scientiarum Biological Sciences
collection Acta Scientiarum Biological Sciences
repository.name.fl_str_mv Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)
repository.mail.fl_str_mv ||actabiol@uem.br
_version_ 1799317396997013504

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