Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/68266 |
Resumo: | Chlorophyllin, a semisynthetic compound derived from chlorophyll, has been a focus in cancer prevention because it exerts important biological activities, such as antigenotoxic, antioxidative and anticarcinogenic activities. The aim of this study was to evaluate the effect of chlorophyllin against benzo[a]pyrene toxicity in HepG2/C3A cells. Cells were co-treated (chlorophyllin plus benzo[a]pyrene) and the cell viability, cell growth kinetics, cell cycle, and apoptosis were evaluated. The mRNA levels of cell cycle and apoptotic genes were analyzed. Our results showed that chlorophyllin reduce the antiproliferative effects of benzo[a]pyrene in a multi-specific manner, restoring the normal distribution of the cell cycle and inhibiting the cell death induced by the xenobiotic. Gene expression analysis showed that benzo[a]pyrene decreased the mRNA levels of genes involved in cell cycle control (cyclins and cyclin-dependent kinases) and apoptosis. Cells co-treated with 200 µM of chlorophyllin and benzo[a]pyrene also showed a downregulation of mRNA levels of the genes, but the expression levels of the gene that encodes to tumor protein p53 were maintained near to control. Thus, chlorophyllin is a good candidate as a chemoprotective agent that mitigates the cytotoxic effects benzo[a]pyrene and, thus, might be a promising tool to prevent liver cancer. |
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Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cellsChlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cellschlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity.chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity.Chlorophyllin, a semisynthetic compound derived from chlorophyll, has been a focus in cancer prevention because it exerts important biological activities, such as antigenotoxic, antioxidative and anticarcinogenic activities. The aim of this study was to evaluate the effect of chlorophyllin against benzo[a]pyrene toxicity in HepG2/C3A cells. Cells were co-treated (chlorophyllin plus benzo[a]pyrene) and the cell viability, cell growth kinetics, cell cycle, and apoptosis were evaluated. The mRNA levels of cell cycle and apoptotic genes were analyzed. Our results showed that chlorophyllin reduce the antiproliferative effects of benzo[a]pyrene in a multi-specific manner, restoring the normal distribution of the cell cycle and inhibiting the cell death induced by the xenobiotic. Gene expression analysis showed that benzo[a]pyrene decreased the mRNA levels of genes involved in cell cycle control (cyclins and cyclin-dependent kinases) and apoptosis. Cells co-treated with 200 µM of chlorophyllin and benzo[a]pyrene also showed a downregulation of mRNA levels of the genes, but the expression levels of the gene that encodes to tumor protein p53 were maintained near to control. Thus, chlorophyllin is a good candidate as a chemoprotective agent that mitigates the cytotoxic effects benzo[a]pyrene and, thus, might be a promising tool to prevent liver cancer.Chlorophyllin, a semisynthetic compound derived from chlorophyll, has been a focus in cancer prevention because it exerts important biological activities, such as antigenotoxic, antioxidative and anticarcinogenic activities. The aim of this study was to evaluate the effect of chlorophyllin against benzo[a]pyrene toxicity in HepG2/C3A cells. Cells were co-treated (chlorophyllin plus benzo[a]pyrene) and the cell viability, cell growth kinetics, cell cycle, and apoptosis were evaluated. The mRNA levels of cell cycle and apoptotic genes were analyzed. Our results showed that chlorophyllin reduce the antiproliferative effects of benzo[a]pyrene in a multi-specific manner, restoring the normal distribution of the cell cycle and inhibiting the cell death induced by the xenobiotic. Gene expression analysis showed that benzo[a]pyrene decreased the mRNA levels of genes involved in cell cycle control (cyclins and cyclin-dependent kinases) and apoptosis. Cells co-treated with 200 µM of chlorophyllin and benzo[a]pyrene also showed a downregulation of mRNA levels of the genes, but the expression levels of the gene that encodes to tumor protein p53 were maintained near to control. Thus, chlorophyllin is a good candidate as a chemoprotective agent that mitigates the cytotoxic effects benzo[a]pyrene and, thus, might be a promising tool to prevent liver cancer.Universidade Estadual De Maringá2023-12-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6826610.4025/actascibiolsci.v45i1.68266Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e68266Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e682661807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/68266/751375156861Copyright (c) 2023 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccess D´Epiro, Gláucia Fernanda RochaSemprebon, Simone CristineNiwa, Andressa Megumi Marques, Lilian Areal Biazi, Bruna Isabela Zanetti, Thalita Alves Felicidade, Ingrid Baranoski, Adrivanio Mantovani, Mário Sérgio 2024-03-01T16:12:04Zoai:periodicos.uem.br/ojs:article/68266Revistahttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/PUBhttps://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2024-03-01T16:12:04Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
title |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
spellingShingle |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells D´Epiro, Gláucia Fernanda Rocha chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity. chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity. |
title_short |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
title_full |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
title_fullStr |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
title_full_unstemmed |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
title_sort |
Chlorophyllin attenuates the effects of benzo[a]pyrene in human hepatoma HepG2/C3A cells |
author |
D´Epiro, Gláucia Fernanda Rocha |
author_facet |
D´Epiro, Gláucia Fernanda Rocha Semprebon, Simone Cristine Niwa, Andressa Megumi Marques, Lilian Areal Biazi, Bruna Isabela Zanetti, Thalita Alves Felicidade, Ingrid Baranoski, Adrivanio Mantovani, Mário Sérgio |
author_role |
author |
author2 |
Semprebon, Simone Cristine Niwa, Andressa Megumi Marques, Lilian Areal Biazi, Bruna Isabela Zanetti, Thalita Alves Felicidade, Ingrid Baranoski, Adrivanio Mantovani, Mário Sérgio |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
D´Epiro, Gláucia Fernanda Rocha Semprebon, Simone Cristine Niwa, Andressa Megumi Marques, Lilian Areal Biazi, Bruna Isabela Zanetti, Thalita Alves Felicidade, Ingrid Baranoski, Adrivanio Mantovani, Mário Sérgio |
dc.subject.por.fl_str_mv |
chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity. chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity. |
topic |
chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity. chlorophyllin; chemoprotection; apoptosis; cell cycle; cytotoxicity. |
description |
Chlorophyllin, a semisynthetic compound derived from chlorophyll, has been a focus in cancer prevention because it exerts important biological activities, such as antigenotoxic, antioxidative and anticarcinogenic activities. The aim of this study was to evaluate the effect of chlorophyllin against benzo[a]pyrene toxicity in HepG2/C3A cells. Cells were co-treated (chlorophyllin plus benzo[a]pyrene) and the cell viability, cell growth kinetics, cell cycle, and apoptosis were evaluated. The mRNA levels of cell cycle and apoptotic genes were analyzed. Our results showed that chlorophyllin reduce the antiproliferative effects of benzo[a]pyrene in a multi-specific manner, restoring the normal distribution of the cell cycle and inhibiting the cell death induced by the xenobiotic. Gene expression analysis showed that benzo[a]pyrene decreased the mRNA levels of genes involved in cell cycle control (cyclins and cyclin-dependent kinases) and apoptosis. Cells co-treated with 200 µM of chlorophyllin and benzo[a]pyrene also showed a downregulation of mRNA levels of the genes, but the expression levels of the gene that encodes to tumor protein p53 were maintained near to control. Thus, chlorophyllin is a good candidate as a chemoprotective agent that mitigates the cytotoxic effects benzo[a]pyrene and, thus, might be a promising tool to prevent liver cancer. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-12-06 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/68266 10.4025/actascibiolsci.v45i1.68266 |
url |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/68266 |
identifier_str_mv |
10.4025/actascibiolsci.v45i1.68266 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/68266/751375156861 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2023 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 45 (2023): Publicação contínua; e68266 Acta Scientiarum. Biological Sciences; v. 45 (2023): Publicação contínua; e68266 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317390493745152 |