Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Scientiarum Biological Sciences |
Texto Completo: | http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/55871 |
Resumo: | Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a significant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents. |
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Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulationsBacillus licheniformis SMIA-2 protease used as an additive in detergent formulationsBacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design.Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design.Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a significant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents.Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a significant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents.Universidade Estadual De Maringá2021-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/5587110.4025/actascibiolsci.v43i1.55871Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e55871Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e558711807-863X1679-9283reponame:Acta Scientiarum Biological Sciencesinstname:Universidade Estadual de Maringá (UEM)instacron:UEMenghttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/55871/751375152717Copyright (c) 2021 Acta Scientiarum. Biological Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessFerreira, Larissa Pacheco Cruz, EricaMartins, Meire Lelis Leal2022-02-17T21:58:46Zoai:periodicos.uem.br/ojs:article/55871Revistahttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSciPUBhttp://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/oai||actabiol@uem.br1807-863X1679-9283opendoar:2022-02-17T21:58:46Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM)false |
dc.title.none.fl_str_mv |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
title |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
spellingShingle |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations Ferreira, Larissa Pacheco Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design. Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design. |
title_short |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
title_full |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
title_fullStr |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
title_full_unstemmed |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
title_sort |
Bacillus licheniformis SMIA-2 protease used as an additive in detergent formulations |
author |
Ferreira, Larissa Pacheco |
author_facet |
Ferreira, Larissa Pacheco Cruz, Erica Martins, Meire Lelis Leal |
author_role |
author |
author2 |
Cruz, Erica Martins, Meire Lelis Leal |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ferreira, Larissa Pacheco Cruz, Erica Martins, Meire Lelis Leal |
dc.subject.por.fl_str_mv |
Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design. Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design. |
topic |
Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design. Bacillus; protease; enzymatic detergent; linear alkylbenzene sulphate; hydrogen peroxide; statistical design. |
description |
Microbial proteases, especially from Bacillus spp., have been widely exploited for a broad variety of applications, such as the improvement of the cleaning efficiency of conventional detergents. In this work, the statistical design of the experiment was used to optimize the concentrations of a three-component mixture: Bacillus licheniformis SMIA-2 protease, Linear Alkylbenzene Sulphate and hydrogen peroxide, in an attempt to prepare an environmentally correct cleaning formulation. The results demonstrated that the combination of 1% (w/v) protease with 1.5% (w/v) LAS and 1% (w/v) H2O2 was effective in removing blood from cloth pieces and that a protease concentration decrease from 1.0% to 0.5% (w/v) would not have a significant impact on percent blood removal if LAS concentrations between 1.5-2.0% (w/v) in combination with lower (<0.5%, w/v) concentrations of H2O2 were used. Thus, the protease from Bacillus licheniformis SMIA-2 can be effectively incorporated into cleaning formulations together with LAS and hydrogen peroxide to formulate more sustainable detergents. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-14 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/55871 10.4025/actascibiolsci.v43i1.55871 |
url |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/55871 |
identifier_str_mv |
10.4025/actascibiolsci.v43i1.55871 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/55871/751375152717 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Acta Scientiarum. Biological Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
publisher.none.fl_str_mv |
Universidade Estadual De Maringá |
dc.source.none.fl_str_mv |
Acta Scientiarum. Biological Sciences; Vol 43 (2021): Publicação contínua; e55871 Acta Scientiarum. Biological Sciences; v. 43 (2021): Publicação contínua; e55871 1807-863X 1679-9283 reponame:Acta Scientiarum Biological Sciences instname:Universidade Estadual de Maringá (UEM) instacron:UEM |
instname_str |
Universidade Estadual de Maringá (UEM) |
instacron_str |
UEM |
institution |
UEM |
reponame_str |
Acta Scientiarum Biological Sciences |
collection |
Acta Scientiarum Biological Sciences |
repository.name.fl_str_mv |
Acta Scientiarum Biological Sciences - Universidade Estadual de Maringá (UEM) |
repository.mail.fl_str_mv |
||actabiol@uem.br |
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1799317397917663232 |